Báo cáo Y học: Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx
Báo cáo Y học: Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx
... the purification and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from an anaerobic bacterium (B. stercoris) in human intestine. The substratespecificity and ... Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium Sung-Woon Hong1, Byung-Taek Kim1, Ho-Young ... suggest that chondroitin ABC and AC lyases have exolytic and endolyticaction patterns.These chondroitin ABC and AC lyases showed optimalactivity at pH 7.0 and 5.7–6.0 respectively, like most of previously...
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Báo cáo khoa học: Purification and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt
... well as acharan sulfate(Table 5). The salt-active acharan sulfate lyase was the mostactive to acharan sulfate. When the salt-active acharansulfate lyase activity for heparan was taken as 100%, ... Fr-b and Fr-c) containing the activity of these enzymes werecollected separately and assayed for the activities degradingacharan sulfate and heparan sulfate. Fr-s had acharansulfate lyase activity, ... acharan sulfate lyase was activated to 5.3-fold by salts(KCl and NaCl). The molecular weight of salt-active acha-ran sulfate lyase was 94 kDa by SDS/PAGE and gel filtra-tion. The salt-active acharan...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
... Lewis-Carl,S.&Kirley,T.L.(1997)Immunolocalizationoftheecto-ATPase and ecto-apyrase (ATPDase) in chicken gizzard and stomach: purification and N-terminal sequence of the stomachecto-apyrase. ... 7.4. On the other hand, the CaADPase activity is 80% of the MgADPase activity at a divalent ion-ADPconcentration of 5 mM(data not shown).The ATPase and ADPase activities of the purifiedchicken ... forward primer,5¢-ATGGAGTATAAGGGGAAGGTTGTTGC-3¢ ,and reverse primer, 5¢-TTGGATTTCCAGAAACACTGGA-3¢.PCR was carried out in a 50-lL reaction mixture containing0.5 lL (from a total of 10 lL) cDNA...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... (Carlsbad, CA, USA).Preparation of specific antibodies against b-amylase and C. sepiumRNase-related protein (CalsepRRP)Polyclonal antibodies were raised against b-amylase and theunglycosylated ... solvent accessible surfaces of the modelledamylase were calculated using theGRASP facilities.RESULTS Purification and partial characterization of the b-amylase from C. sepiumrhizomesSDS/PAGE of ... starch, (b) many plant b-amylasesare spatially separated from their presumed substrate (i.e.starch), and (c) inbred lines of rye lacking the abundantendosperm b-amylase germinate normally...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
... Purification and biochemical characterization of some of the properties of recombinant human kynureninaseHarold A. Walsh and Nigel P. BottingSchool of Chemistry, University of St Andrews, ... metabolism.Rapid progress in the pathophysiology of human diseaseshas always been hampered by the availability of human tissue, aesthetics, and ethical considerations. The principleaim of ... 4). This is a major difference of human kynureninase from other mammalian enzymes, suchas rat hepatic kynureninase, and may imply that previousreports of weak activity withL-kynurenine in...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... biantennary and triantennary N-glycans that are terminated by a2 ,3-linkedsialic acids, a high number of which are O-acetylated. About1/3 of the glycans carry sulfate at N-acetylglucosamine units of ... water and analysed by MALDI-TOF MS.Phosphatase treatmentDry N-glycan samples were dissolved in 50 mMammoniumbicarbonate, and 1 U of calf intestinal alkaline phosphatase(New England Biolabs, ... like papain and ficin but they had noeffect on trypsin, a serine proteinase. Wolffish kininogencarried a2 ,3-sialylated biantennary and triantennary N-gly-cans with extensive sialic acid O-acetylation....
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Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt
... Purification and characterization of three galactose specific lectins from Mulberry seeds (Morussp.)Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and Nurul AbsarDepartment of Biochemistry, ... NID-Galactose – 25 IN-AcetylD-glucosamine 110 – NIMethyl -a- D-galactopyranoside – 30 IMethyl-b-D-galactopyranoside – 20 IMSL-3 N-Acetyl-galactosamine 110 – NID-glucosamine-HCl 110 – NILactose ... contains about 38% carbohydrate,32% fat and 15% protein [8]. This paper describes the purification and characterization of three galactose-specificlectins from the seeds of mulberry.MATERIALS AND...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... polymerase(Stratagene) was used to amplify vanXYCusingpAT704astemplate with primers A (5¢-GCTAGGTCTCAATGAACACATTACAATT-3¢)andB(5¢-TATGGAATTCTCATGCGAACTGCCTCA-3¢) that included BsaIandEcoRIrestriction ... the VanC phenotype, VanXYCmust specific-ally hydrolyseD-Ala-D-Ala with minimal activity againstD-Ala-D-Ser, a very different type of specificity.VanXYC, VanX-type and VanY-type enzymes ... primers C (5¢-CTCAGGATCCAACACATTACAATTGATCAATA-3¢)andD(5¢-CACTAAGCTTTCATGCGAACTGCCTCAC-3¢) that included BamHI and HindIII restriction sites, respectively (underlined).The product was purified,...
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Báo cáo Y học: Purification, characterization and subunits identification of the diol dehydratase of Lactobacillus collinoides pot
... The enzymerequired the adenosylcobalamin coenzyme for catalyticactivity and the Kmfor the cofactor was 8 lM. Inactivation of the enzyme was observed by both glycerol and cyano-cobalamin. ... 1,2-ethanediol and glycerol to propionaldehyde, acetaldehyde and 3-hydroxy-propionaldehyde, respectively [1]. This dehydration reactionis the first step of an anaerobic metabolism pathway. Thealdehyde ... Diol dehydratase purification and characterization (Eur. J. Biochem. 269) 5733containing the maximal diol dehydratase activity werecombined and used for the characterization of the enzyme.After...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... various divalent metal cations and EDTA on theactivity of SAP1 and SAP2. The standard assay A was used except thatthe phosphoenolpyruvate concentration was subsaturating (4 mM).Enzyme activity ... kDa), BSA (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (29 kDa). AP was also visualized inacrylamide gels by staining for AP activity. SDS/PAGE wasFig. 1. Separation of secreted AP ... Electrophoretic patterns of CNBr cleavage fragments of SAP1 and SAP2. CNBr cleavage fragments were prepared from gel slicescontaining 3 lgofSAP1(lane1)andSAP2(lane2)andanalyzedonan SDS/14% PAGE minigel as...
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