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Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt

Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt

Báo cáo Y học: Comparison of starch branching enzyme I and II from potato ppt

... branching enzyme activity on amylose and amylopectin with the starch iodine assayAmylose (type III, Sigma) and amylopectin (Sigma) from potato were typically dissolved at 10 mg:mL21in 0.5 MNaOH. ... and 2Department of Food Science, Swedish University of Agricultural Sciences, Uppsala, SwedenThe in vitro activities of purified potato starch branching enzyme (SBE) I and II expressed in Escherichia coli ... activity of potato SBE I and II Phosphate has been reported to increase the branching activity of SBE I and II from wheat [25] and SBE I from potato [26]. The activity assay shown in Fig. 1 wasperformed...
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Báo cáo y học: "Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors"

... adenovirus and its infected cells may be a primary cause of this functional limitation. The antigenicity of adenoviral vectors, which induce the host immune response, consist of directly injected ... (ADhBMPs) are significantly limited in immunocompetent animals. It is unclear what role expression of viral proteins and foreign proteins transduced by adenoviral vectors play in the host immune response ... volumes induced by ADhBMP6 were significantly smaller in immunocompetent animals than in immunodeficient animals. In contrast, bone formation induced by ADhBMP9 was similar in AN and immunocompetent...
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Báo cáo y học: "Comparison of Classical and Clozapine Treatment on Schizophrenia Using Positive and Negative Syndrome Scale of Schizophrenia (PANSS) and SPECT Imaging"

... brain (Figure 1). With these brain maps, physicians are able to identify certain patterns of brain activity in different psychiatric and neurological problems. Basically, in a typical brain ... Raese JD. Hypofrontality and cognitive impairment in schizophrenia: dynamic single-photon tomography and neuropsychological assessment of schizophrenic brain function. Biol Psychiatry 1990; 27(4):377-399. ... Sharafi obtained MD from Kerman Medical University, and a specialty in psychiatry from Iran University of Medical Sciences, where he worked in the research areas of schizophrenia, mood disorders...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)

... primarily on its potential as a diagnostic assay for relevant coronary stenosis. Resting ECG analysis, including 12-lead ECG, typically has significantly less sensitivity in detecting ischemia ... tools for initial screening and diagnosis in clinical practice. The low sensitivity and specificity of these methods makes them less than ideal diagnostic and prognostic indicators of CAD, however ... device ap-pears to provide a high sensitivity and specificity for the identification of relevant CAD, as diagnosed by coronary angiography, in patients with a low or high pre-test risk of...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

... regulation of stress kinases and apoptosis [5,6].Based on their sequence structure, catalytic activitiy,immunogenicity, substrate specificity and sensitivity toinhibitors, the mammalian GSTs form six ... 0.20Hydroxyethyl disulfide 0.11Table 2. Inhibitor sensitivities of recombinant SmGSTO. Inhibitorsensitivities are presented as I 50 and were calculated in standard reac-tion conditions. Values in ... reached. Finally, SmGSTO showed differentialsensitivity to several GST inhibitors tested (Table 2).Among them, it is interesting to note the inhibitory activity of CDNB which is considered as...
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Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc

Tài liệu Báo cáo Y học: Characterization of a cloned subtilisin-like serine proteinase from a psychrotrophic Vibrio species doc

... University of Iceland; and 2Department of Biochemistry, Science Institute, University of Iceland,Reykjavik, IcelandThe gene encoding a subtilisin-like serine proteinase in thepsychrotrophic ... conditions as VPRwtboth enzymesdisplayed identical inactivation behaviour, indicating thatthe same disulfide(s) are involved in maintaining theintegrity of active protein structure in both wild-type ... form.AfeatureoftheVibrio-proteinase is its sensitivity totreatment with dithiothreitol. In the presence of 10 mMdithiothreitol at 25 °C, the activity of enzyme is decreasedto about one-quarter of its...
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

... Sun2 and Zhong-ming Qian31Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, China;2Department of Chemistry and Open Laboratory of Chemical Biology, ... K .Y. , Wong, W.B., Loh, T.T., Sadler, P.J. &Sun, H. (2001) Interactions of bismuth with human lactoferrin and recognition of the Bi(III)-lactoferrin complex by intestinal cells.Biochemistry ... confocalmicroscopic studies indicated that Yb2-Tf is likely to enterthe cells in a way similar to Fe2-Tf, and at the same timealmost without affecting the function of Tf. It will beinteresting...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

... quicker,high-yield procedure for the p u rification of this flavoenzyme.In addition, the physical and chemical characteristics, theactivity on glycine and sarcosine, and the substrate specifi-city of the ... its stability ismaximal in the 7.0–8.5 pH range. A comparison of amino-acid sequence and substrate specificity indicates t hat GO h assimilarities to other flavoenzymes acting on primary amines and ... impair either theenzymatic activity or the coenzyme binding.The purification of the His-tagged chimeric GO and, for comparison, that of the wild-type enzyme is reported inTable 2 . From this table...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

... membranes containing 25 mol% PtdSer in the presence of 10 mMCa2+obtained by evaluation of association and dissociation phases (I) and equilibrium binding data (II) as described in Materials and methods.kon(MÆs))1koff(s)1) ... employingequilibrium dialysis established the existence of about 10Ca2+-binding sites, at least three of which mediate cooper-ative binding [23–26]. By studies of the binding of divalentcations ... of Clinical Chemistry, University Hospital Malmo¨, Lund University, Malmo¨, Sweden;2Department of BiophysicalChemistry, Lund University, Lund, SwedenThe interaction of blood coagulation...
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Báo cáo Y học: Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium influx during rat Sertoli cell development pptx

Báo cáo Y học: Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium influx during rat Sertoli cell development pptx

... participation of syndecans in signal transduction mechanisms while that of glypicans is still poorly understood.In the mammalian testis, Sertoli cells are epithelia somaticcells associated, by ... glycosylphophatidylinositol (GPI)anchors [1,2]. Although the primary structure of the glypi-cans is only marginally conserved (about 30% identity), thereis a strict conservation of 14 cysteine residues ... coreceptor with integrins [17]. The variablecytoplasmic region unique to syndecan-4 interacts with thecatalytic domain of PKCa and stimulates its activity [18].These observations strongly suggest...
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