Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
... linking the active site tyrosine residue of topoisomerase I with the 3¢ terminus of DNA in topo-isomerase I DNA complexes which accumulate duringtreatment of cancer with camptothecin. In yeast, ... torsion-al strain of DNA during replication, repair and transcrip-tion processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleavedstrand [1]. During ... parameters of human TDP whichhydrolyzes the phosphodiester bond that links the Topo I enzyme and its DNA substrate during catalysis in the presence of Topo I inhibitors such as camptothecin. Inhib-itors...
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Tài liệu Báo cáo Y học: Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 potx
... competitiveinhibitor against dihydroxyacetone phosphate andNAD(P)H. However, NAD(P)+acted as a competitiveinhibitor against NAD(P)H and as a noncompetitiveinhibitor against dihydroxyacetone ... distinct features fromeubacteria and eukaryotes, including the unique stereo-chemical backbones of phospholipids in their cellularmembrane. The core lipid of the phospholipids andglycolipids ... °C in 1.5 mL cuvettes containing 1.2 mL reactionmixture and initiated by the addition of 10 lLofenzymesolution. Control reactions were carried out using the samereaction mixture without enzyme. ...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... (rutin)Quercetin-4¢-GlAKaempferol-3-GlcIsorhamnetin-3-GlcFlavanones Naringenin-7-GlcRha (naringin)Hesperetin-7-GlcRha (hesperidin)Dihydrochalcones Phloretin-7-Glc (phlorizin)Secoiridoids OleuropeinAnthocyanidins Cyanidin-3-Glc ... similarity and 83.6 % ami no-acid similarity with gu inea pig C BG, and showed homol-ogy w ith domains III and I V o f mammalian LPH (56 and57% amino-acid similarity, respectively) and with the putative ... the speci®city of the human CBG with r espect to the glyconeand aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing a broad r ange of dietary xenobiotic...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... consistent with titration of the catalytic histidine, H438(440). The persistence of activity in the presence of the protonated form of the catalytic histidineis inconsistent with the generally ... catalyticsite (i. e. a catalytic triad of Ser-His-Glu, an oxyanion hole, ap-cation-binding site, and an acyl-binding pocket) are the same in AChE and BuChE (Fig. 1). The acyl-bindingpocket, which is ... catalysis. Rather, the appearance of activity under conditions in which the catalytic histidine is protonated indicates a change in the mechanism of BuChE and AChE. Work is in progress toprobe the...
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Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf
... cells is the samesize as the polypeptide identified in crude extracts of spinachleaves by antibodies raised against the recombinant protein. The size of this protein is very similar to t hat of ... forFru-2,6-P2ase activities in the liver isoform of t he mamma-lian enzyme are conserved in the same relative positionswithin the spinach leaf sequence (Fig. 1). Similarly, 17 of the 21 residues identified ... forhydrolysis of Fru-2,6-P2, and is inhibited by Fru-6-P andP i , although the affinities for these inhibitors differ fromthose of the Fru-2,6-P2ase activity of the bifunctional enzyme. The protein...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... rod linker,which resulted in the disintegration of the rod complexes. The two bilins present in b-phycocyanin give a greatercontribution to the UV absorption than the s ingle bilin of the other ... components, and the individual biliproteins, either with or without attachedlinkers, are obtained [6]. The relative stability of biliprotein-linker complexes varies among the biliproteins f rom differ-ent ... masses of the proteins in each HPLC peak. The peakseluting within 25 min represented linker p roteins, while the four main peaks between 28 and 33 min were the phyco-cyanins and allophycocyanins...
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Báo cáo Y học: Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system docx
... first obvious difference in theircatalytic properties to be identified. Kinetic data wereconsistent with the inhibition due to binding of the secondsubstrate. We discuss the inhibition mechanism ... contribu-tion of the aliphatic chain of fatty acids to their binding, ascompared with P450BM3. In other words, the interaction of P450foxy with the carboxylate anion of fatty acids at the entrance ... Kinetic analysis of hydroxylation of saturated fatty acidsby recombinant P450foxy produced by an Escherichia coliexpressionsystem7Tatsuya7Kitazume1, Akinori Tanaka1, Naoki Takaya1,...
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Báo cáo y học: "A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis"
... sensitivities found in our study were higher in comparison to the study in question but specificity is lower. This situation may be related with the phase of the brucella infection. In a study ... agglutination tests have an important role in the diagnosis of brucellosis. Main antigenic structure which is imported in the diagnosis of bru-cellosis is the smooth lipopolysaccharide structure ... sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were...
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Báo cáo y học: "Comparative study of serum Na+ and K+ levels in senile cataract patients and normal individuals"
... currently an associate professor in Department of Biochemistry and Nutrition in Iran University of Medical Sciences. He is interested in study of nutrition and trace elements in human blood. Manuchehr ... Imamian obtained BSc in Chemistry from Payam-Nour University of IRAN in 1996 and MSc in Biochemistry from Iran University of Medical Sciences in 2001. His areas of research focus on nutrition ... He is currently an associate professor in Department of Ophthalmology, Iran University of Medical Sciences, with research interests in epidemiology in ophthalmology. Issa Nourmohammadi obtained...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... iron and lipids [14,56,57] may berelevant in a variety of liver diseases. It is anticipatedthat iron overload in hepatic cells in clinical situations in vivo might cause changes in lipid metabolismand ... accumulation and(c) increased the prooxidant state and the level of oxidatively modified proteins. These results are in accordance with previous in vitro and in vivo studies of hepatic iron loading ... tooxidatively modified proteins containing the aldehydeadduct acrolein (a gift from K. Uchida8, Nagoya University,Nagoya, Japan). Rabbit anti-mouse and goat anti-rabbitIgs, fluorescein isothiocyanate...
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Từ khóa: báo cáo y họcbáo cáo y học cổ truyềnbáo cáo khoa học y họcbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ