Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
Báo cáo Y học: Conformational analysis by CD and NMR spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia potx
... spectroscopy of a peptide encompassing the amphipathic domain of YopD from Yersinia Tobias Tengel1, Ingmar Sethson1 and Matthew S. Francis21Departments of Organic Chemistry and 2Molecular Biology, ... study, we designed synthetic peptides encompassing theC-terminal amphipathicdomain of YopD. A solution structure of YopD 278)300, a peptide that stronglyinteracted with LcrH, was obtained by NMR ... putative amphipathic domain. This strategy wasadvantageous because full length YopD is susceptible toaggregation [20] and the amphipathic domain is clearlybiologically relevant [10,13]. The...
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Báo cáo Y học: Conformational analysis of opacity proteins from Neisseria meningitidis ppt
... CEACAM1 were amplified from CEACAM cDNA (gift from M. Kuroki, FukuokaUniversity, Fukuoka, Japan) with primer pair 5¢-ATCATATGCAGCTCACTACTGAATCCATGCC-3¢ and 5¢-ATCGGGATCCCTAACTCACTGGGT TCTGTATTTC-3¢ and ... Microbiology, University of Amsterdam/AMC, Amsterdam, the Netherlands;3Department of MolecularMicrobiology and 4Department of Pharmaceutics, Utrecht University, Utrecht, the NetherlandsOpacity-associated ... function as an Opa receptor as has beenshown for the gonococcal and meningococcal Opa proteinsanalyzed to date. Both OpaB128 and OpaJ129 boundspecifically to the N-terminal domain of CEACAM1. Asthe...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot
... expressionin branchial epithelial cells of Japanese eel,Anguilla japonicaKentaro Miyamoto1, Nobuhiro Nakamura1, Masahide Kashiwagi1, Shinji Honda1, Akira Kato1,Sanae Hasegawa2, Yoshio Takei2 and ... of euryhaline fishes. The proteins may contribute to remode-ling of the gill architecture and its maintenance by targeting,for degradation via the proteasomal pathway, a group of regulatory and ... cells. The lamellar chloride cellsare considered to play a pivotal role in freshwateradaptation as they appear in freshwater and disappearin seawater [28,29]. The chloride cells are mainly located...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... concentrated to 10% of the originalvolume of the nuclear extract by trichloroacetic acidprecipitation. The concentrated sample was analysed by SDS/PAGE (10% polyacrylamide).Amino acid sequencing of ... Hepaticcirrhosis and neuronal degeneration are the most debilita-ting symptoms of WD and are caused by the impairment of biliary copper excretion and the accumulation of toxicconcentration of ... nuclear extracts. We purified and characterized the MREa-binding activity, which consisted of two polypeptides of approximately 70 and 82 kDa.N-terminal and internal amino acid sequencing and...
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Báo cáo Y học: Kinetic analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by an Escherichia coli expression system docx
... analysis of hydroxylation of saturated fatty acids by recombinant P450foxy produced by anEscherichia coliexpressionsystem7Tatsuya7Kitazume1, Akinori Tanaka1, Naoki Takaya1, Akira ... 140P450foxy (Fusarium)b1200 900 2,000 a Kitazume et al. [14].bNakayama et al. [13].Fig. 2. Apparent substrate specificity of rP450foxy for saturated fattyacids. Enzymatic activity was assayed at a ... free FAD and FMN remarkably accelerated the specific activity of rP450foxy as observed with the native enzyme [28]. Dodec-anoic acid hydroxylase and NADPH-cytochrome c reduc-tase were assayed...
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Báo cáo Y học: Functional analysis of the rat bile salt export pump gene promoter Regulation by bile acids, drugs and endogenous compounds potx
... nucleotide sequence, as well as a systematic structural and functional analysis of the 5¢ flanking region of the Bsepgene. The 5¢ deletional analysis of the Bsep promoter revealed a region from nucleotide ... bases in bold) and sense (5¢-GCTAGAGCTGGACTTTATTCCATTGAAATA-TAAGCAAACAGTG-3¢) oligonucleotide of the IR-1 element was used in a temperature cycling reactionas described in the manufacturer’s protocol ... activity they are capable of causing cholestasis by the reduction of canalicular bile acid transport capacity. Incontrast with b-estradiol the anti-estrogen tamoxifen stimu-lated Bsep promoter activity...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... events that involves ac-tivation of many genes and synthesis of various pro-teins. The importance of several apoptotic pathways such as mitochondrial pathways and death receptor pathways has been ... There are two major intracellular apoptosis-signaling cascades; mitochondrial pathways and death receptor pathways [4]. Mitochondrial pathways are regulated by various proapototic and antiapoptotic ... Bid and Bad. It may also enhance apoptosis by inhibiting the expression of Bcl-2 and by modulat-ing phosphorylation of JNK. Both mitochondrial and stress-activated apoptosis pathways played...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... agarose gels. 3. Results Mutational analysis of hCDC4 in AML In this study the common exons 2 to 11 and the three known transcript variants of exon 1 of hCDC4 (Figure 1A) were analyzed by ... cellular pathways leading to chromosomal instability [12]. As the disruption of the above described cellular oncogenic pathways also plays an important role in hematological malignancies ... interested whether mutations of hCDC4 can also be observed in Acute Myeloid Leukemia (AML) or high risk Myelodysplastic Syndrome (MDS). Therefore we carried out a mutational analysis of the hCDC4 gene...
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Báo cáo y học: " Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression"
... real-time RT-PCR as described above. RNA extraction and array analysis. For each array sample, the RNA was prepared from the purified thymocytes. The thymocytes were processed by using the ... genes which changed the most with age, we uploaded all of our array data into the array analysis program of the NHGRI (http://arrayanalysis.nih.gov). Table 3 lists the top genes that were up- ... Abcam (Cambridge, MA). The antibody to beta-actin was from Sigma-Aldrich (St. Louis, MO). The HRP-conjugated secondary antibody for the beta-actin was from Amersham (Piscataway, NJ). Bands...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... plasmid specific primers (M13F-cccagtcacgacgttgtaaaacg- and M13R-agcggataacaatttcacacagg) were from Life Technologies, Inc. All other chemicals were of analy-tical grade or higher quality.Animals, ... through a myotoxic/cytolytic site distinct from the catalytic site, as alreadydescribed for the inhibition of myotoxicity by heparin [44].At least in the case of B. asper mt-II [50] and of a Lys49PLA2 from ... was fractionated by ion-exchange chromatography (Fig. 1A) and the heterogeneousDM64 was obtained as the ascending portion of the mainacidic peak. This sample was further purified by affinitychromatography...
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