... (Bio-Rad)andthen10mLofanti-eRBCCserum(diluted1 :10 in NaCl/Pi) was applied to the column and incubate at 4 °C for 24 h. The bound antibody was eluted with 10 mL of 10 0 mMglycine (pH 2.5) and dialyzed ... proteins. The second motif or the B-boxis defined by the consensus sequence CHC3H2and bindsone zinc atom. Members of the RBCC protein familyinclude PML [1] , TIF1 [2], KAP -1 [3], the MID1 gene product ... lLofabuffercontaining 0 .1 lgofrabbitE1,1lgofE2,1lg of GSt-Ub,25 mMTris/HCl (pH 7.5), 12 0 mMNaCl, 2 mMATP,1mMMgCl2,0.3mMdithiothreitol, 1 mMcreatine phos-phokinase, 10 0 lMMG -13 2, and 10 0 ng of GSt-eRBCC.E2s...