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Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf

Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf

Báo cáo Y học: Interaction of the GTS1 gene product with glyceraldehyde3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae pdf

... 269) 3569 Interaction of the GTS1 gene product with glyceraldehyde-3-phosphate dehydrogenase 1 required for the maintenance of the metabolic oscillations of the yeast Saccharomyces cerevisiae Weidong ... previously [14 ,16 ]. GTS1[ DKN]and GTS1[ C5 3Y] inserted into pAUR 112 were transformedinto gts1D and the transformants were named pACGTS1[DKN]/gts1D and pACGTS1[C5 3Y] /gts1D, respectively.Preparation of TDH-deleted ... severely shortened when the wild-type GTS1 was overexpressed in gts1D (pYXGTS1/gts1D)(Fig. 3). When gts1D cells overexpressed Gts1p-C5 3Y orGts1p-DKN, the duration of the oscillations was lengthenedFig....
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot

Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot

... The specificity of the AFP immuno-reaction was verified by omitting the primary antibody.RESULTSScreening for interaction with hexamerin receptorUsing the yeast two-hybrid s ystem and ABP 13 0, ... wasadded for hybr idization overnight at 42 °C. The slides werewashed according to the following scheme: 3 · 10 min with 4 · NaCl/Cit; 2 · 10 minwith2· NaCl/Cit; 1 0 min with 0 .1 · NaCl/Cit; 10 min with ... three wash s teps with NaCl/Pi, the sections were fixed for 2 min in 2% paraform-aldehyde and then for 10 min in 10 mMTris/HCl /1 mMEDTA (pH 7.4). After a 1- h prehybridization, the heat-denatured...
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Báo cáo y học:

Báo cáo y học: "Elevated plasma homocysteine is positively associated with age independent of C677T mutation of the methylenetetrahydrofolate reductase gene in selected Egyptian subjects"

... (0.83 -1. 45) 1. 1 ±0.23 (0.6 -1. 76) 1. 2 ±0. 31 (0.83 -1. 32 )1. 1. 2 Creatinine (µmol/L) (79.6 -10 9.6) 10 4 10 .9 (10 6 -14 1.4 )12 5.2 18 .4∗ (79.6-2 21) 136.5 ± 44.4∗ tHcy (µmol/L) (5 .1 -36) 17 .99 ± 9.76 (13 -76) ... for the thermolability of MTHFR. Actually, the frequency of the mutated allele was quite high, depending on the ethnic group analyzed [18 - 21] . Some authors suggested that the homozygosity for ... 2004 1: 18 1 -19 2 19 2 Figure 3. The effect of the T allele on the plasma homocysteine in the different studied groups. Bar chart illustrating the effect of the T allele on the level of homocysteine...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot

... in the absence of chaotropic agents, with the exception of the wavelength of the minimum ( 215216 instead of 218 nm)and very different to the spectrum of the same preparationpurified in the ... significantly modify both the triple helical conformation of the trimeric species (Fig. 3 B)and the thermal stability, with the relevant exception of N-acetylation with acetic anhydride for the reasonsmentioned ... probable that glycosylation of hydroxylysinewill block the binding. However, the glycosylation pattern of Hyl residues is known for CB7 [50] but not for CB10.Aliquots of both CB10 and CB7 have...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf

... 2.2) · 10 3(3.7 ± 0.2) · 10 )2(4.6 ± 1. 3) · 10 )6(9.4 ± 1. 4) · 10 )6Gla–EGFN(4.5 ± 1. 1) · 10 4(3.8 ± 0.2) · 10 )2(8.4 ± 2 .1) · 10 )7 (1. 7 ± 0.3) · 10 )6Gla–EGFN,C(6.7 ± 2 .1) · 10 4(4.3 ... inresidues 1 11 of the Gla-domain [8]. Moreover, NMRstudies of the Ca2+-free form of the Gla-domain establishedthat the metal ion binding translocated the residues thatconstitute the hydrophobic ... described by the following equations. The rate equation for the bivalentanalyte model:A þ B )*kon ;1 koff ;1 AB ð2ÞAB þ B )*kon;2koff;2AB2ð3Þwhered½B=dt ¼À2kon ;1 ½A½Bþkoff ;1 ½ABÀkon;2½AB[B]þ...
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Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf

Báo cáo Y học: Interaction of plasminogen activator inhibitor type-1 (PAI-1) with vitronectin Characterization of different PAI-1 mutants pdf

... ±Mutant 5 (D109 -11 2) D F109-H 112 10 000 + NTMutant 6 (A 114 -11 5) F 114 A and R 115 A 57 000 + NTMutant 7 (A 114 11 8) F 114 -R 118 vs. AAAAA 20 800 + +Mutant 8 (M8) F 114 -R 118 vs. AAAAA and D68G 7 700 + ... i n some of t he generated PAI -1 mutants. P73A, single amino acid exch an ge of P73 to alanine ;A 114 11 8, exchange of the sequence 11 4FRLFR 118 to ®ve a lanines; D109 11 2, deletion of the four ... silver-stained. 200 U o f PAI -1, 1 10 U o f Q123K, 50 U of P73A and of A 114 11 8 were applied; alternatively 1. 4 lg of ( inactive ) M 4 a nd 1 0 U of PAI -1 were used. 18 8 N. Arroyo De Prada et al. (Eur....
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Báo cáo Y học: Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid pdf

Báo cáo Y học: Interaction between p21-activated protein kinase and Rac during differentiation of HL-60 human promyelocytic leukemia cell induced by all-trans-retinoic acid pdf

... pathway. Cell 81, 11 37 11 46. 15 . Minden,A.,Lin,A.,Claret,F.X.,Abo,A.&Karin,M. (19 95)Differential activation of ERK and JNK mitogen-activated pro-tein kinases by Raf -1 and MEKK. Cell 81, 11 37 11 57. 16 . ... on day 5, although they showed very low superoxidegenerating activity (15 ± 5 nmol O2–per 10 min per 10 7cells). The dormant cells exhibited a very low level of O2–production both with ... 95% homogeneityusing thrombin cleavage from a glutathione affinity matrix.Approximately 1. 18 g of cytosol was mixed and incubated with 10 mL of 2¢,5¢-ADP-Sepharose beads for 12 h at 3 °C. The beads...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot

Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot

... (Bio-Rad)andthen10mLofanti-eRBCCserum(diluted1 :10 in NaCl/Pi) was applied to the column and incubate at 4 °C for 24 h. The bound antibody was eluted with 10 mL of 10 0 mMglycine (pH 2.5) and dialyzed ... proteins. The second motif or the B-boxis defined by the consensus sequence CHC3H2and bindsone zinc atom. Members of the RBCC protein familyinclude PML [1] , TIF1 [2], KAP -1 [3], the MID1 gene product ... lLofabuffercontaining 0 .1 lgofrabbitE1,1lgofE2,1lg of GSt-Ub,25 mMTris/HCl (pH 7.5), 12 0 mMNaCl, 2 mMATP,1mMMgCl2,0.3mMdithiothreitol, 1 mMcreatine phos-phokinase, 10 0 lMMG -13 2, and 10 0 ng of GSt-eRBCC.E2s...
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Báo cáo Y học: Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells pptx

Báo cáo Y học: Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells pptx

... corresponding to tyrosine-phosphorylation sites inSLP-76 and Vav were synthesized. The peptides used in thispaper include: SLP -Y1 13 : WSSFEEDDYESPND, SLP- Y1 28: QDGEDDGDYESPNE, SLP -Y1 45: APVEDDADYEPPPS, ... in the absence or presence of tyrosine-phosphorylated peptides or phosphotyrosine(P -Y) . The peptides correspond to tyrosines 11 3, 12 8 and 14 5 in SLP-76 and tyrosines 17 4 and 692 in Vav. SLP- 3Y ... inproximity with the ZAP70 tyrosine kinase, the mainphosphate-donor and thus activator, of these signalingproteins. These interactions are then of importance for the activation of both the MAP...
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Báo cáo y học:

Báo cáo y học: "Weight loss, leukopenia and thrombocytopenia associated with sustained virologic response to Hepatitis C treatmen"

... (57%) with the mean age of 52.5 years (Table 1) . Fifty six percent were infected with genotype 1, 16 % with genotype 2 and 28% with genotype 3. Clinical char-acteristics, relevant laboratory data ... 50 year 2.5 (0.57 -10 ) 0.24 1. 38 (0.23-8.33) 0.73 Genotype Non -1 15 .33 (1. 82 -12 9 .18 )0. 01* 15 .22 (1. 55 -14 9.72) 0.02* Weight loss more than 5 kilograms 3.86 (1. 05 -14 .82) ... P20RR 011 0 91, from the National Center for Research Resources, National Institutes of Health. The contents are solely the re-sponsibility of the authors and do not necessarily represent the official...
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