... Science,Sports and Culture of Japan (14350433, 14045229), a grant from theScience and Technology Incubation Program in Advanced Region by JST (Japan Science and Technology Corporation), and the SasakawaScientific ... total residualactivities of 52% and 57%, for 0.2 mgÆmL)1lysozyme, wererecovered by the addition of 100 mMspermidine and spermine, respectively. On the other hand, the residualactivity was ... theTm by 151.9–2.7 °C. Gly exhibited the best results, judging by the increased Tm. The decreased Tmvalue, as conferred by polyamines, implies that they bind to the unfolded mole-cules. DH and...