... vectors generate
efficient knockdown of target proteins
To examine the efficiency of generation of RNAi for
pSM155 and pSM30, we examined the knockdown of
expression of firefly Luciferase (Luc). HeLa ... pmiR30, and the pair
of oligos used to generate pmiR155, into the XhoI and
EcoRI sites of pEGFP-N1-Intron, respectively. A pair of
oligos including cohesive ends and a s...
... Expansion of Multi-Word Terms for Indexing and Retrieval
Using Morphology and Syntax*
Christian Jacquemin Judith L. Klavans
Institut de Recherche en Informatique Center for Research
de ... combination of parsing over a seed term
list coupled with derivational morphology
to achieve greater coverage of multi-word
terms for indexing and retrieval. Fina...
... ‘none’,
and the mass error tolerances for precursor and fragment
ions were typically set to 0.15 u for qTOF MS ⁄ MS and
50 p.p.m. and 1 u, respectively, for MALDI-TOF ⁄ TOF MS
data. The formation of ... from
tropoelastin isoform 2; Fig. 1), PQGLAG, and PQGKAG
were generated by taking the coordinates of the prodomain
(YPFALAPT, residues 96–103) of the crystallized proform...
... stimulation with LP and LPS.
Expression patterns of RGS1 and RGS2 after
activation of TLR3 and TLR9
To investigate the expression levels of RGS1 and
RGS2 after activation of other TLR, BMDM were
stimulated ... were stimulated with FSL-1, a ligand for the
TLR2 ⁄ 6 heteromer, and LPS, a ligand for
TLR4 ⁄ MD2. After various culture times, mRNA was
isolated and the express...
... FSBA COFRADIC. The structures of
ATP and its reactive homolog FSBA are
shown in (A) and (B), respectively. The
COFRADIC reaction sorting for SBA-labeled
peptides is shown in (C).
COFRADIC and protein ... the
availability of massive numbers of DNA sequences,
recent developments in MS, and bioinformatics tools
that link DNA and protein sequences to information
generated by dif...
... stimuli and provides a
mechanism for prompt termination of the pr otein s ynthesis.
These data a dd to the growing body of information
supporting a preponderant role of p38 in the regulation of
gene ... forms o f RhoA, Cdc42 and Rac;
to Dr J.H. Han (The Scripps Institute, CA) for providing CaMKK3
and CaMKK6 const ructs and to D r A. Morrison for providing u s with
theact...
... source of user-created content,
and a flurry of recent research has aimed to under-
stand and exploit these data (Ritter et al., 2010; Shar-
ifi et al., 2010; Barbosa and Feng, 2010; Asur and
Huberman, ... 2010).
than for Standard English text. For example, apos-
trophes are often omitted, and there are frequently
words like ima (short for I’m gonna) that cut across
traditio...
...
Conversion of the WordNet KB was also
straightforward. WordNet files in Prolog are part
of the standard WordNet distribution. Therefore,
the bulk of the task involved routine reformatting ... corpus of 1330 sentences (on
the subject of our NLU system) in order to give a
quantitative description of the contribution of our
lexicon and semantics servers. Our corpus
contain...
... School of Pharmaceutical Sciences, University of Tokyo, Japan
The a3b1 integrin is an adhesion receptor for extracellular
matrix proteins including isoforms of laminin, and the
changes of its ... a
promiscuous receptor for a variety of extracellular matrix
proteins such as fibronectin, collagen, and laminin-1 (a
prototype of laminin), and for cell surface counter-ligand...
... 1B, lanes 4 and 7), and, in the
case of Cox4-1, Cox6a and Cox7a, processing of their
presequences.
We next tested the assembly of newly imported
radiolabeled complex IV subunits using BN-PAGE.
Radiolabeled ... assembly
and human disease, and also to assess the possible
involvement of Surf1 in the formation and assembly of
the LSI complex. BN-PAGE and western blot anal...