... primers 5¢-AAATATAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3¢. The final ratio of targetcells was determined by counting the number of coloniesretaining the target genes.AcknowledgementsThis ... 67–79.8 Takesako K, Ikai K, Haruna F, Endo M, ShimanakaK, Sono E, Nakamura T, Kato I & Yamaguchi H(1991) Aureobasidins, new antifungal antibiotics.Taxonomy, fermentation, isolation, and properties.J ... G-proteinsignaling (Fig. 1A) [11–13]. As the outputs appear asvarious mating responses, including global changes intranscription, a reporter gene assay and mating selec-tion are available (Fig. 1A) ...