... N-terminal signal peptide (amino acids1–36), we retained amino acid 36, an alanine, ratherthan using amino acid 37, a lysine, because it isknown that, after a post-translational processing of ... pre-pro-tyrosinase (Fig. S1) can be dividedapproximately into three domains: a twin argininetranslocase (TAT) signal peptide, a core domain con-taining the two copper-binding motifs and a C-termi-nal ... theirbiotechnological application.AbbreviationsL-DOPA, L-3,4-dihydroxyphenylalanine; TAT, twin arginine translocase.FEBS Journal 277 (2010) 2083–2095 ª EMPA, Swiss Federal Laboratories for Materials Testing and...