... 13320M)1Æcm)1[16].All measurements for unliganded protein were performedin buffer A (10 mM Tris/HCl, 150 mM NaCl, 2 mMdithiothreitol, pH 8.0), but for the complex of protein withTc, buffer ... process, R is thegas constant, and A is equal to 4.0 for monomer or for nondissociated dimer [19].Circular dichroismCD measurements were performed on a Jasco-710 spectro-polarimeter equipped ... transition parameters for TetR and complex of TetR with Tc at various concentrations. The buffer for TetR was10 mM Tris/HCl, 150 mM NaCl, 2 mM dithiothreitol, pH 8.0. The buffer for TetR + Tc was...