... Wieds Vej 10C,DK-8000 Aarhus C, Denmark.Fax: + 45 86123178, E-mail: co@mbio.aau.dkAbbreviations: PAPP-A, pregnancy-associated plasma protein-A;PAPP-Ai, variant of PAPP-A with 29-residue insert ... incubated(24 h) with medium from mock-transfected cells (lane 1), with mediumfrom cells transfected with murine PAPP-A cDNA (lanes 2–3), with murine PAPP-Ai cDNA (lanes 4–5), or with human PAPP-A cDNA(lane ... ofPAPP-Ai is only about 10% of the activity of PAPP-A(Fig. 4A). A similar time course experiment was performed with IGFBP-5 as the substrate. Surprisingly, PAPP-A andPAPP-Ai degraded IGFBP-5 with...