... 4 shows the binding isotherm for ibuprofenbinding to tHSA–heme-Fe(II)-NO. Analysis of the dependence of the molar fraction of the ibuprofen-bound tHSA–heme-Fe(II)-NO (Y) on the ibuprofenconcentration ... acid; HSA, human serum albumin; HSA–heme-Fe, human serum heme-albumin; HSA–heme-Fe(III), ferric HSA–heme-Fe;HSA–heme-Fe(II), ferrous HSA–heme-Fe; HSA–heme-Fe(II)-NO, nitrosylated HSA–heme-Fe(II); ... ibuprofen binding to HSA–hemehas been reported to induce the hexa-coordination of the heme Fe atom [18,21,23]. Thus, peroxynitrite can-not bind to the heme Fe(III) atom of hexa-coordinatedHSA–heme-Fe(III),...