Báo cáo Y học: The a1b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids potx
Báo cáo Y học: The a1b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids potx
... 211 The a1 b1 contact of human hemoglobin plays a key role in stabilizing the bound dioxygen Further evidence from the iron valency hybrids Jun pei Yasuda1, Takayuki Ichikawa1, Mie Tsuruga1, ... 4).Similarly, Shaanan [31] reported the stereochemistry of the iron -dioxygen bond in human HbO2by single-crystalX-ray analysis. In the a chain, the distance between Ne of His (E7) and the terminal ... than its a1 b1 counterpart in HbO2.At all rates, the present spectral examinations clearlyindicate that the formation of the a1 b1ora2b2 contact suppresses remarkably the a cidic a utoxidation...
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Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx
... open-chain tetrapyrroles are produced from biliverdinthat is a product of heme degradation. Accordingly, the chlorophyll and phycobilin syntheses share the pathway of protoporohyrin synthesis from ... d-aminolevulinic acid [1,2].Phycobilins work as the main photoreceptor of photosyn-thesis in procaryophyta, cyanobacteria and other primitiveeucaryotic algae. Phycobilin synthesis branches from ... Ishikawa, A. , Kawashima, K.,Kimura,T.,Kishida ,Y. ,Kohora,M.,Matsumoto,M.,Matsuno, A. , Muraki, A. , Nakazaki, N., Shimpo, S., Sugimoto, M., Taka-zawa, M., Yamada, M., Yasuda, M. & Tabata, S....
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... 48h. The corresponding karyotypes (A2 to E2) were analyzed by the ISIS imaging software (Metasystems GmbH, Altussheim, Germany). In all experimental groups analyzed, no chromosomal translocations ... temperature tolerance of hESC with respect to their survivability, undifferentiated state and chromosomal normality. Physiologically, mammalian cells are naturally adapted to a constant body ... immunostaining, but were instead kept continuously in culture through a number of serial passages. Their appearance was virtually indistinguishable from non-temperature exposed hESC (data not...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... expression profiling of human erythrocyte could be an important key for under-standing the machinery of anucleate protein synthesis and its meaning in the pathophysiology of diseases. Acknowledgements ... protein synthesis in human platelets by megakaryo-cyte-derived mRNAs 19. The finding of RNA in anu-cleate cells like erythrocytes support the idea of nu-cleus independent protein synthesis and ... in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA...
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Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... phosphorylation of TPH. In this experiment, the amount of the PKA-catalytic subunit and that of the otheragents added were optimized using rat phenylalaninehydroxylase (PAH, molecular mass of 55 ... contaminated the preparation) was clearlyphosphorylated (Fig. 3A) . When an effort was made toenhance the autoradiograph image (32P panel in Fig. 3B), a faint and diffuse signal appeared at a ... are described in Materials and methods. Sizemarkers are shown in kDa at left. Arrows indicate the estimated position of TPH and the arrowheads indicate contaminating protein in the PKApreparation...
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Báo cáo Y học: Substrate specificity of human kallikrein 2 (hK2) as determined by phage display technology docx
... reveal thatan arginine appears in 40 clones at the P1 site and only onepeptide is cleaved at a lysine. Among the substrateshydrolysed at an arginine, 11 different amino acids appearedat the ... selectiveinhibitors for cancer chemotherapy as well as accelerate the discovery of new targets. Phage display has alreadyopened new avenues in kallikrein research that may further reinforce the role ... fluorescencereleased from the beads as the CFPs were cleaved in theirsubstrate linker. Percentage of hydrolysis was calculated as the ratio of released CFPs to the initial amount of CFPs bound to the beads...
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Báo cáo khoa học: Cytosol–mitochondria transfer of reducing equivalents by a lactate shuttle in heterotrophic Euglena docx
... respectively. These data suggestedthat in Euglena, oxalate also slightly inhibited enzymes(probably pyruvate kinase and preceding enzymes) involved in the glycolytic pathway, although glycolysis was ... availability and that iLDHs play a key role in the transfer of reducing equivalents from the cytosol to the res-piratory chain (lactate shuttle).Keywords: energy metabolism; lactate metabolism; NAD+-lactate ... the stationaryphase in the three culture media.Exhaustion of both externalD-andL-lactate correlatedwith the start of the stationary phase (Fig. 4B). Arrival at the stationary phase in the...
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Báo cáo y học: "An unusual case of gout in the wrist: the importance of monitoring medication dosage and interaction. A case report"
... body not only the lower limb. Patients should be aware of the signs and symptoms of an acute gouty attack andbe made aware that changes in certain medication dosages may precipitate an attack. ... dayslater and were read by a radiologist. The radiographsrevealed well-marginated juxta-articular bony erosions at the radial styloid process and the dorsal rim of the distalradius with soft ... intensepain, redness and swelling of the joint. Examination willreveal a red, swollen, and extremely tender joint. Naturalhistory of an acute attack ranges from a few days to a fewChiropractic &...
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Báo cáo y học: "Spindle Cell Lipoma of the Hypopharynx"
... Immunohistochemical examination for pathological diagnosis indicated a spindle cell lipoma. Conclusions: Laryngeal and hypopharyngeal lipomas are rare forms of benign laryngeal neoplasms. Their asymptomatic ... laryngoscopy did not reveal any masses at bilateral pyriform sinuses, and the mucosa had a natural appearance. However, a mass with a stalk was detected in the side of the mouth when the patient retched. ... subdermal, while rarely at the dermal tissue layer. They are commonly found at the dorsal neck, nape, shoulders, and the back1. Spindle cell lipoma was first defined by Enzinger and Harley in...
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Báo cáo y học: "Aplasia and Agenesis of the Frontal Sinus in Turkish Individuals: A Retrospective Study Using Dental Volumetric Tomograph"
... above a line tangential to the supraorbital margin. Frontal sinus aplasia was also defined by an oval-shaped sinus with the lateral margin medial to a vertical line drawn through the middle of ... By the age of 8 years, the top of the frontal sinuses is at the level of the orbital roof, and by the age of 10 years, the sinuses extend into the vertical portion of the frontal bone. The ... light was centered at the level of the sinus, indicating the optimized center of the re-construction area. In addition, the head position was adjusted in such a way that the hard palate was par-allel...
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