... D289AMY2 and D206TAA, E230TAA, and D297TAA). The invariant Y5 1AMY2 and Y8 2 TAAare at subsite )1asareH 92 AMY2 and H 122 TAA;M 52 AMY2(M53AMY1)andW83TAAare at subsite )2; T94AMY2(C95AMY1 )at subsite- 5 ... A1 45AMY2, F180AMY2, K1 82 AMY2, W206AMY2,S208AMY2, Y2 11AMY2, H288AMY2, Q294AMY2, M296AMY2 and Q35TAA, H 122 TAA, R204TAA, K209TAA, H210TAA, G234TAA,D340TAA, R344TAA) ... 20 02 Barley a- amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the b a loop 2 of the catalytic (b /a) 8-barrel and is critical for activity and substrate specificity Haruhide...