Báo cáo khoa học: Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities pptx

Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx

... [21]. Theseconsiderations indirectly reinforce the argument that the basic domain should be exposed at the surface of the Tat-containing recombinant proteins and call other reasonsto explain the ... in the internalization process [4]. Thus, sterichindrance of the heterologous protein itself could reduce the exposure of these shorter peptides to cellular structures, and therefore, reduce the ... not alter the uptake of the Tat CPP. Thesebiochemical evidences con®rmed a pathway for the e ntry of the Tat peptide unrelated to the HS proteoglycan receptors.Internalization of the Tat CPP...

Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classiﬁcation pptx

... annealing at the beginning and end of the cDNA and sequenced.Sequence analysis and homology modelingDNA and amino acid sequences were processed, analyzed and aligned with the vector nti software ... mammals and othervertebrates, it is less certain in the case of invertebrates and basal metazoans. Nevertheless, the amino acidsequence of the Sycon group I PLA2demonstrates that the ancestor ... To determine the relative enzymatic activity of UcPLA2, the slopes of the curves were calculated from the obtained measurements, the background ﬂuorescence was subtracted, and the resulting...

Báo cáo khoa học: Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities pptx

... Biochem. 270) 3749 Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities Philippe ... CXC chemokines GCP-2 and ENA-78 by human gelatinase B, of human GCP-2 by neutrophil collagenase, and of the single mouse counterpartof these chemokines, named mouse GCP-2/LIX, by mouse gelatinase ... Comparison of the cleavage of IL-8 by human gelatinase B and of mouse GCP-2 by the mouse enzyme. (B) Comparison of the velocities of the processing of the human chemokines IL-8, GCP-2 and ENA-78....

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... 30821065 and 30870559 from the China National Nature SciencesFoundation, 2006CB910703 from the Ministry ofSciences and Technology of China, and 07dz05907from the Committee of Sciences and Technology ... one of the prodeath proteins in the Bcl-2 fam-ily, and interacts with prosurvival Bcl-2 family mem-bers to inactivate them [32]. Akt-1 phosphorylates Badat Ser136, inactivating Bad and thereby ... adipocytes, the translo-cation of Bax from the cytoplasm into the mitochon-dria, cytochrome c release and cleavage of caspase-3were all observed (Fig. 4B–D).To conﬁrm the involvement of Bcl-2 and...

Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... complex The interaction on the one hand between MT1-MMP and GRASP55 and on the other hand betweenGRASP55 and furin led us to hypothesize thatGRASP55 could potentially play a role in the forma-tion ... the interaction between TGF-a ICD and the GRASP55PDZ1 domain [37]. Phosphorylation has been found to modulate interactions between PDZ domains and their ligands [47–49]. The afﬁnity of the Erbin–ErbB2 inter-action ... in the formation of the MT1-MMP–furin complex; therefore,disruption of the interaction between GRASP55 and furin should perturb the activation of pro-MT1-MMP and reduce MT1-MMP activity at the...

Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx

... heterogeneousgroup of peptides with respect to their primary and secondary structures, their antimicrobial potentials, their effects on host cells, and the regulation of their expression. Most antimicrobial ... cells, keratinocytes, and epithelialcells. When released into the extracellular milieu, theyexert their antimicrobial activity directly by attacking the microbe membrane, and in the intracellular ... settings. Moreover, differentialproteolytic processing can modulate their activity and, by extension, their ability to modulate immunity [17].Consequently, the combination of defense peptidesproduced...

Tài liệu Báo cáo khoa học: Plasticity of S2–S4 speciﬁcity pockets of executioner caspase-7 revealed by structural and kinetic analysis pdf

... block the potential exosite and their removal starts the process of executioner caspase activation, as well as the expo-sure of the exosite. Clearly, further studies are requiredto identify the ... caspase-7. The peptide inhibitors makemost of their contacts with the residues from the p10subunit, except for those with the catalytic dyad(Cys186 and His144) and the residues anchoring the aspartate ... active sites and pos-sibly serves as an exosite for caspase-7. The size and charge of the central cavity differs between caspase-3 and 7. Furthermore, the two extended N-termini of the large subunits...

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

... evaluated by the statistical potentialused in the present study. In the case of the RNA muta-tions to the SRP complex, the mutated RNA residuesare located in a double-stranded region of RNA, and donot ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scoredthese sequence-variant structures with the distance-dependent potential function.Figure 2 shows the ... score; the blue line represents the rank of thesesequences using the distance-dependent potential. The points ineach colored line are sorted independently by rank; the x-axis is the sort order. The...

Tài liệu Báo cáo khoa học: Pyrimidine-speciﬁc ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc

... of the program, setting the highest level of optimization. The quality of the mod-els and their stereochemical properties were analyzed using the programs procheck [65] and prosaii [66], and the best ... reference, the position of the sugar and of the metal in the crystallo-graphic template 1YOE. The two ligands were modelled ineach binding site of SsCU-NH, and then the structure of the resulting ... hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and 4.2 min and 6.6 and 3.9 minrespectively. The amount of...

Tài liệu Báo cáo khoa học: A novel tachykinin-related peptide receptor of Octopus vulgaris – evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide ppt

... neuro-peptides such as the TK and TKRP family is correla-ted with the evolution and divergence of the nervoussystem and their biological roles, and thus, functionalcharacterization of oct-TKs and oct-TKRPs ... oct-TKRPR, TKRPR and TKR family. The amino acid sequence of oct-TKRPR was aligned with those of the TKRPRs (UTKR, NKD, STKR, and DTKR), and TKRs (mouse NK1R, NK2R and NK3R, and Ci-TK-R) usingCLUSTALW. ... insects and several other invertebrates, avariety of biological activities of TKRPs, such as the contraction of the hindgut and oviduct, depolarizationor hyperpolarization of several neurons, and...

Xem thêm