Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx

Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx

Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx

... Consecutive additions of Glc- NAc and Gal to Galb1-4GlcNAcb-pNP by b3GnT and b4GalT. (B) Consecutive additions of GlcNAc and Gal to Galb1-4Glcb-pNP by b3GnT and b- D -galactosidase or b4GalT. (C) N- acetylglucosaminy- lation ... mg) based on the acceptor from 3 and UDP-Gal. 1 Hand 13 C-NMR data of 2 and 4 are summarized in Table 1. Preparation of GlcNAcb1-3Galb1-4Gl...
Ngày tải lên : 31/03/2014, 07:20
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Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... is not as efficient as its tandem- configured relative [16], its successful construction and the demonstration of its functionality supports the idea of creating novel RNA catalysts by rational ... functional RNA, for example, sig- nalling aptamers and aptazymes [8–10]. Work in our laboratory has focused on the rational design of functional RNA, in particular on the deve...
Ngày tải lên : 20/02/2014, 01:20
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... amounts of Akazara scallop TnC. Lanes h and m, ATnI-52K; lanes i and n, ATnI-19K; lanes j and o, ATnI 1)128 ; lanes k and p, ATnI 130)252 ; lanes l and q, ATnI 232)292 ; lane r, Akazara scallop ... then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca 2+ ; lanes b and e, in the presence of TnC and the absence of Ca 2+ ; lanes c and f, in the...
Ngày tải lên : 20/02/2014, 01:20
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... hand, the determined catalytic constants are apparent ones, with varying relative contributions of individual rate constants and their discussion is not straightforward. An important detail of ... biocatalysts often requires transformations of substrates not encountered in nature under reaction condi- tions differing from physiological ones. Protein design aimed at rational optimizati...
Ngày tải lên : 16/03/2014, 16:20
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Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc

... recombinant cN-II assay. Accordingly, an alter- ation o f t his r atio for a mutant was considered as being caused either by an alteration of the K m value for one of the two s ubstrates or by a variation ... thrombin cleavage site. The p ro tein concentration w as determined according to Bradford [30], using BSA as a standard. The molar concentration of the enzymes was det...
Ngày tải lên : 16/03/2014, 18:20
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot

... Hirabayashi 2 1 National Food Research Institute, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan 2 Research Center for Medical Glycoscience, National Institute of Advanced Industrial ... the configuration at the hemiacetal carbon of galactose may affect the dissociation constants. By contrast, because chemical-shift changes upon the addition of sugars at s...
Ngày tải lên : 23/03/2014, 04:21
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Tài liệu Báo cáo khoa học: "Three BioNLP Tools Powered by a Biological Lexicon" doc

Tài liệu Báo cáo khoa học: "Three BioNLP Tools Powered by a Biological Lexicon" doc

... percentage of the terminological words and derivational relations (such as the word retroregulate and the derivational relation retroregulate → retroregulation) in our lexicon that are also ... …” IL/NP IL-2/NN-BIOMED -/- 2/CD mediated/VVD IL-2-mediated/UNKNOWN IL/NP 2/CD IL-2/NN-BIOMED BioLexicon mediated/VVD mediate/VVP mediate/VV of/ IN mediated/VVN -/- -/- mediated/V...
Ngày tải lên : 22/02/2014, 02:20
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Báo cáo khoa học: "Machine Aided Error-Correction Environment for Korean Morphological Analysis and Part-of-Speech Tagging" pptx

Báo cáo khoa học: "Machine Aided Error-Correction Environment for Korean Morphological Analysis and Part-of-Speech Tagging" pptx

... '~(saram)/ncn+da'un/ncpa', '2 ,-7, (hag'gyo)/ncn+da'un/ncpa'. Correction rule can apply to the many kinds of word phrase; while manual log is concerned about only one instance ... transformation rules dealing with agglutinative characteristics of Korean, and also extends the tagger by using specific transforma- tion rule considering the lexic...
Ngày tải lên : 08/03/2014, 05:21
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Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan ppt

Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan ppt

... because of its nonseasonal availability and easy main- tenance. They were obtained from the coastal region of Kovalam, Chennai, India. They were maintained in the laboratory at a temperature of 28 ... Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan Unit of Biochemistry, Department of Zoology, University of Madras, Tamil Na...
Ngày tải lên : 16/03/2014, 14:20
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Báo cáo khoa học: Alpha 1-antichymotrypsin/SerpinA3 is a novel target of orphan nuclear receptor Nur77 potx

Báo cáo khoa học: Alpha 1-antichymotrypsin/SerpinA3 is a novel target of orphan nuclear receptor Nur77 potx

... (transactivation function 1, AF-1), a DNA-binding domain, and a ligand-binding domain. Nur77 has no specific ligand discovered hith- erto. The crystal structure of the ligand-binding domain of Nurr1, another ... SerpinA3 (1 : 50 dilution in NaCl/P i ) containing 3% BSA at 4 °C overnight, washed abundantly in NaCl/P i , and incubated for 1 h with the TRITC-conju- gated secondary ant...
Ngày tải lên : 23/03/2014, 07:20
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