Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... Consecutive additions of Glc-
NAc and Gal to Galb1-4GlcNAcb-pNP by b3GnT and b4GalT. (B)
Consecutive additions of GlcNAc and Gal to Galb1-4Glcb-pNP by
b3GnT and b-
D
-galactosidase or b4GalT. (C) N- acetylglucosaminy-
lation ... mg) based on the acceptor from 3 and UDP-Gal.
1
Hand
13
C-NMR data of 2 and 4 are summarized in
Table 1.
Preparation of GlcNAcb1-3Galb1-4Gl...
... is not as efficient as its tandem-
configured relative [16], its successful construction and
the demonstration of its functionality supports the idea
of creating novel RNA catalysts by rational ... functional RNA, for example, sig-
nalling aptamers and aptazymes [8–10].
Work in our laboratory has focused on the rational
design of functional RNA, in particular on the
deve...
... amounts of Akazara scallop TnC. Lanes h and m, ATnI-52K; lanes
i and n, ATnI-19K; lanes j and o, ATnI
1)128
; lanes k and p, ATnI
130)252
; lanes l and q, ATnI
232)292
; lane r, Akazara scallop ... then run on SDS ⁄ PAGE. Lanes a and d, in
the absence of both TnC and Ca
2+
; lanes b and e, in the presence of TnC and the absence of Ca
2+
; lanes c and f, in the...
... hand, the determined catalytic constants are apparent
ones, with varying relative contributions of individual rate
constants and their discussion is not straightforward.
An important detail of ... biocatalysts often requires transformations of
substrates not encountered in nature under reaction condi-
tions differing from physiological ones. Protein design
aimed at rational optimizati...
... recombinant cN-II assay. Accordingly, an alter-
ation o f t his r atio for a mutant was considered as being
caused either by an alteration of the K
m
value for one of the
two s ubstrates or by a variation ... thrombin cleavage site.
The p ro tein concentration w as determined according to
Bradford [30], using BSA as a standard. The molar
concentration of the enzymes was det...
... Hirabayashi
2
1 National Food Research Institute, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan
2 Research Center for Medical Glycoscience, National Institute of Advanced Industrial ... the
configuration at the hemiacetal carbon of galactose
may affect the dissociation constants.
By contrast, because chemical-shift changes upon
the addition of sugars at s...
...
percentage of the terminological words and
derivational relations (such as the word
retroregulate and the derivational relation
retroregulate → retroregulation) in our lexicon
that are also ... …”
IL/NP
IL-2/NN-BIOMED
-/-
2/CD
mediated/VVD
IL-2-mediated/UNKNOWN
IL/NP
2/CD
IL-2/NN-BIOMED
BioLexicon
mediated/VVD
mediate/VVP
mediate/VV
of/ IN
mediated/VVN
-/-
-/-
mediated/V...
... '~(saram)/ncn+da'un/ncpa', '2
,-7, (hag'gyo)/ncn+da'un/ncpa'.
Correction rule can apply to the many kinds
of word phrase; while manual log is concerned
about only one instance ... transformation rules dealing with
agglutinative characteristics of Korean, and also
extends the tagger by using specific transforma-
tion rule considering the lexic...
... because of its nonseasonal availability and easy main-
tenance. They were obtained from the coastal region of
Kovalam, Chennai, India. They were maintained in the
laboratory at a temperature of 28 ... Molecular mechanisms regulating molting in a crustacean
Halagowder Devaraj and Ayithan Natarajan
Unit of Biochemistry, Department of Zoology, University of Madras, Tamil Na...
... (transactivation function 1,
AF-1), a DNA-binding domain, and a ligand-binding
domain. Nur77 has no specific ligand discovered hith-
erto. The crystal structure of the ligand-binding
domain of Nurr1, another ... SerpinA3 (1 : 50 dilution in NaCl/P
i
)
containing 3% BSA at 4 °C overnight, washed abundantly
in NaCl/P
i
, and incubated for 1 h with the TRITC-conju-
gated secondary ant...