Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx

Báo cáo khóa học: Characterization of novel structural features in the lipopolysaccharide of nondisease associated nontypeable Haemophilus inﬂuenzae pptx

... have been identiﬁed in those strainsthat are responsible for most of the steps in the biosynthesis of the oligosaccharide portions of the LPS molecules. The inner core of H. in uenzae LPS has ... from the nasopharynx to the middle ear via the eustachian tube, often in the presence of viral respiratoryinfections [5]. Whether all colonizing strains of H. in uenzaeare equally capable of ... within these bacteria. Aproponderance of glycoforms containing two PCho unitsmay enhance the ability of these strains to colonize the nasopharynx.Modiﬁcation of LPS by addition of terminal...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... localize the ligandbinding sites in the leukocyte b2integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi-mately 200-amino-acid intervening domain (I domain),which ... anti-ICAM-4antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efficient inhibition of the Fig. 10. Specific binding of purified recombinant I domain GST fusionproteins to ... tested in cellularbinding assays to find out possible differences in binding of ICAM-4 and the other two ICAMs for the I domains.Many of these mAbs displayed similar inhibitory profiles in assays investigating...

Báo cáo khoa học: Characterization of a prokaryotic haemerythrin from the methanotrophic bacterium Methylococcus capsulatus (Bath) ppt

... NorwayHaemerythrin proteins comprise a family of O2-carrry-ing proteins mainly found in a few phyla of marine invertebrates. Members of this family differfrom haemoglobin and haemocyanin in that they ... from the PCR fragmentampliﬁed with the primers used in the cloning of MCA0715. The arrowhead indicates the 540 bp transcript, corresponding to the predicted length of the MCA0715 transcript. The ... haemerythrinFEBS Journal 272 (2005) 2428–2440 ª 2005 FEBS 2433C-terminal end of helix III, pointing towards the inside of the bundle, and Cys128 was located to the randomcoil of the C-terminal end of...

Báo cáo khoa học: Characterization of a digestive carboxypeptidase from the insect pest corn earworm (Helicoverpa armigera) with novel speciﬁcity towards C-terminal glutamate residues pptx

... favoured over lysine [4]).Speciﬁcity is primarily determined by interaction of the sidechain of the C-terminal amino acid of the substrate with abinding pocket on the enzyme, with amino acid 255(human ... majority, and by 20 min all, of the original 50 kDa protein had been digested, with anincrease in staining of the bands of  36 kDa and 13 kDa. On further incubation with trypsin the 13 kDapolypeptide ... to allow the PCR product to beinserted into the Pichia expression vector pGAPZaBwith the N-terminus of the proprotein in- frame and adjacent to the cleavage point of the yeast a-mating factor...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... mutantproteins. This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG is not ... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine ... concentration. The dissociation constants, Kdvalues, enthalpies of binding and stoichiometries weredetermined from the binding isotherm by ﬁtting a 1 : 1binding model to the data using the software...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... cyanidin;100 nm bilirubin and 100 nm biliverdin. Under the condi-tions of the assay, bilirubin is freely soluble in the buffer[58]. The presence of these inhibitors in the assay mediummay interfere ... antibody A in the presence of increasing concentrations of bilirubin. BSP uptake wasassayed to track the progress of the antibody-inducedinhibition. Figure 5A shows that increasing bilirubinconcentrations ... kAand k0are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2and k1are the rate constants of the inhibition of the bilitranslocase–bilirubin...

Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... by the C domain of the initiation module of dapto-mycin. That DptE and DptF are involved in the lipidation process of daptomycin was ﬁrst shown byMiao et al. [1]. In their work, the daptomycin ... is GLDSV,indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether DptF is the putative ... [37].Interestingly, in the case of the lipopeptide surfactin,neither an acyl CoASH ligase-like domain nor an ACPcould be identiﬁed within the biosynthetic gene clusterusing bioinformatic tools...

Báo cáo khoa học: Deamidation of labile asparagine residues in the autoregulatory sequence of human phenylalanine hydroxylase potx

... carbonyl of Asn by the nitrogen of the peptide group linking the Asn to the following residue [4–6]2. As hydrolysis of thisintermediate may occur on either side of the imidenitrogen, the Asp ... containing the regulatory and catalytic domains [15].Fig. 2. Stereo picture of the three Asn residues in the N-terminal autoregulatory sequence of rPAH. Figure based on the crystal structure of the ... however, the lower S/N ratio of the spectra in Fig. 4E than in 4B that has an effect on the accuracy of the deconvoluted spectrum.Lability of the Asn residues in the autoregulatorysequence The 28-residue...

Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

... of the MR151-derived strain with the crtIa deletion (MR841) com-pletely lose the red color typical of colonies from the parental strain, suggesting that the absence of CrtIablocks the synthesis ... compilation ª 2007 FEBS 4307 in- frame deletion of crtIa in M. xanthus strain MR151.This strain contains a mutation, carR3, which renders the expression of the carB operon independent of external stimulation ... on the expression of downstream genes[12]. To clarify the possible function of crtIa in the carotenoid synthesis pathway, we generated anFig. 1. Schematic of the initial carotenoid biosynthesis...

Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx

... are the pH and Eh7 in the experiments with the addition of inhibitor (the arrow indicates the time of addition); curves (C) and (D) are the pH and Eh7 in the control experiment.Thiol groups in ... addition of NEM or AMdiS to a reducingculture of L. lactis rapidly increased the Eh7andrestored the initial Eh7value (Fig. 3). These data show the implication of thiol groups in the decrease in ... in modulating the thiol–disulﬁde ratio in the medium andon the cell surface in response to a variation in the intracellular pH [15]. GSH is not synthesized in L. lactis [28] and a decrease in...

Xem thêm