... Comparative analysis of the site-specific N-glycosylation
of human lactoferrin produced in maize and tobacco plants
Be
´
ne
´
dicte Samyn-Petit
1
, Jean-Pierre ... France;
2
Meristem Therapeutics, Clermont-Ferrand, France
We have compared the site-by-site N-glycosylation status of
human lactoferrin (Lf) produced in maize, a monocotyle-
don, and i...
... simultaneously
interacts with the main phosphate of the substrate [32].
The closer positioning of the N-terminus to the main
protein body is essential for the activity and, together with
the 87–104 ... almost
linear and can be obtained by calculating the slope of the
curves of Pyr-ONC (M23L) vs. time. From the rates of
cyclizationshowninTable1,itcanbeinferredt...
... probably induced by streng-
thening of the interaction between the structural TnC-
binding site and the C-domain of TnC accompanying
Ca
2+
binding to site IV of TnC. In vertebrate tropo-
nin, the ... TnC-bind-
ing site interacts with the C-domain of TnC in both
the relaxed and contractile states, which plays a role
in maintaining the structural integrity of...
... assessed in more detail,
Rout et al. interpreted the significance of the identification
of the other proteins in three ways: firstly, there are proteins
that according to the literature are known NPC interactors,
e.g. ... which, depending
on the approach, contain different kinds of information
exceeding the mere identification of proteins. These
comprise of studies on di...
... of Jacalin-related lectins
Comparative analysis of carbohydrate-binding properties
of two tandem repeat-type Jacalin-related lectins,
Castanea crenata agglutinin and Cycas revoluta leaf lectin
Sachiko ... was
determined by measuring the amount of uncoupled protein
in the above wash fraction by the method of Bradford [33].
The resulting lectin-columns were slotted int...
... modulation of chromatin structure
and transcription mediated by nucleosome binding of
PARP-1 [12], and in the determination of specificity in
a retinoid signalling pathway via direct modulation of
Mediator ... embryo-
genesis.
PARP-3
The protein domain structure of PARP-3 is very
similar to PARP-2, featuring a small DNA-binding
domain consisting of only 54 amino acids...
... mechanism of antitumour
action of BS-RNase is based on the ability of the protein
to resist the neutralizing action of the cytosolic RNase
inhibitor, a resistance due to the dimeric structure of the
enzyme. ... devoid of toxicity as native, dimeric
BS-RNase (data not shown).
It is noteworthy that in the inactive MCM, MCA and
MAE monomers Cys31 and Cys32, the...
... 1). These findings can be explained by the coor-
dination of l-arginine in the active site of VioC
(Figs 4, 5 and 6B). As the stereochemistry of the
Ca-atom is crucial to allow the manifold interactions
between ... methylene group in the side chain of
l-arginine, as exemplified by the binding and catalytic
turnover of l-homoarginine. Concerning l-canavanine
turno...
... presence of the phenol.
Interestingly, these constants resemble the reciprocal of the
nitrite binding constants to the proteins.
According to Schemes 1 and 2, the nitrating species
produced by the ... monitoring the absorbance changes at
322 nm due to the formation of the oxidative coupling
dimer of 4. The initial rates were determined from the linear
part o...
... generating name variations of
terms in GENA and the UMLS meta-thesaurus
1
.
Table 3 shows the size of the original database and
the number of entries expanded by name varia-
tions. Terms in MEDLINE ... IE and text mining in biomedical science has
focused mainly on MEDLINE. In the present pa-
per, we target all articles indexed in MEDLINE at
the end of 2004...