Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx
Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx
... Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry Jesper J. Hedberg*, ... conditions.Ó FEBS 20 03 GSNO reduction by human alcohol dehydrogenase 3 (Eur. J. Biochem. 270) 1251determined by MS/MS. The MS/MS spectra of the peaks atm/z 33 7, [GSNO]H+,andm/z 33 9 are displayed in ... see Yang et al. [34 ].Scheme 1. Transformation of GSNO to glutathione sulfinamide by ADH3.Ó FEBS 20 03 GSNO reduction by human alcohol dehydrogenase 3 (Eur. J. Biochem. 270) 12 53 major product is...
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Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx
... Genomics, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, WI 537 06 134 4, USAFax: +1 608 262 37 59Tel: +1 608 2 63 934 9E-mail: markley@nmrfam.wisc.edu(Received ... GappedBLAST and PSI-BLAST: a new generation of proteindatabase search programs. Nucleic Acids Res 25, 33 89 34 02. 23 Falk A, Ek B & Rask L (1995) Characterization of anew myrosinase in Brassica ... (glycoanaly-sis by the three axes of MS and chromatography): aweb application that assists structural analyses of N-gly-cans. Trends Glycosci Glycotechnol 15, 235 –251. 38 Yamamoto S, Hase S,...
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Báo cáo khoa học: Reduction of a biochemical model with preservation of its basic dynamic properties doc
... This fits their localization aroundthe central part of glycolysis. The importance rankingmatches the polar phase plane plot analysis above, andthe conclusions of Madsen et al. [31 ]. This is ... system has been thoroughly characterizedin terms of both biochemistry and dynamics [16– 23] ,and this has led to a number of mathematical models of this system [4,24 30 ].Our 20D model [30 ] is a ... mostimportantly by the ATPase reaction. The flux controlexhibited by the glycerol branch is also a manifestation of demand control, because increased flux in the gly-cerol branch decreases the...
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Báo cáo khoa học: Inhibitor of nuclear factor-kappaB alpha derepresses hypoxia-inducible factor-1 during moderate hypoxia by sequestering factor inhibiting hypoxia-inducible factor from hypoxia-inducible factor 1a ppt
... was knocked down,Asn8 03 hydroxylation was enhanced, and this wasattenuated by FIH knockdown (Fig. 4A, right panel).On the other hand, in 0.5% oxygen, Asn8 03 was negli-gibly hydroxylated, and ... RNAs were isolated from HEK2 93 cells by Trizolreagent (Invitrogen). RNA was quantified by measuringabsorbance at 260 nm. RNAs (1 lg) were reverse tran-scribed at 48 °C, and the resulting cDNAs ... were analyzed by immunoblotting. Asn8 03- hydroxylated HIF-1a was detected using an antibody against hydroxylated asparagine (N8 03- OH). (C, D) HEK2 93 cells were transfected as described above and...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... Q4: 3. 82% Q4: 9 .37 %Q3: 93. 40%Q3: 85.00%Q3: 90.48%Q3: 92.44%Q4: 2.89%Q4: 9.94%Q4: 3. 90%Q4: 2.81%Q1: 3. 80% Q1: 0 .34 % Q1: 0.48%Q2: 3. 76%Gate: R1Q1: 0.54%Q2: 3. 17%Q2: 31 .49%Q1: 0. 43% Q2: ... cellsMichalis D. Mantzaris1, Epameinondas V. Tsianos2and Dimitrios Galaris11 Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece2 First Division of Internal ... mechanisms of lipotoxicity in nonalcoholic fatty liver disease. SeminLiver Dis 28, 36 0 36 9. 3 Schaffer JE (20 03) Lipotoxicity: when tissues overeat.Curr Opin Lipidol 14, 281–287.4 Adams LA, Sanderson...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... cyto-plasm of Picrophilus. The kinetics of NADPHdegradation was followed by measuring the rate of decrease of its absorbance at 34 0 nm over the pHrange 3. 6–7.0 and at 40, 60 and 80 °C. The measuredhalf-life ... albumin as a standard.Assay for glucose dehydrogenase activity andenzyme kineticsGlucose dehydrogenase activity was assayed spectrophoto-metrically by measuring the increase of absorption at 34 0 ... themetabolism of this organism has adapted to this cir-cumstance.Because of the unusually low intra- and extracellularpH of Picrophilus cells and their milieu, respectively,certain enzymes from this...
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Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
... leukae-mias and ovarian cancer [16,26] support a role forcholinesterases in carcinogenesis. This notion is givenweight by the aberrant expression and structuralchanges of acetylcholinesterase and ... [27], the role of acetyl-cholinesterase in apoptosis [ 23] , and the downregula-tion of cholinesterases in metastasized lymph nodes[29], as well as in colorectal [30 ] and lung [31 ] can-cers.Despite ... of cholinesterases in human kidney has notbeen studied yet. The present research was intended topartially fill this gap by examining the levels of cholin-esterase activity and mRNAs in human...
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Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... P450reductase (P450red, EC 1.6.2.4).(Received 4 November 2002, revised 23 January 20 03, accepted 10 February 20 03) Eur. J. Biochem. 270, 134 9– 135 5 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 08.xpheromonal ... Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursorRole of recombinant cytochromeb5and P450 reductasePenny ... nm.ResultsIdentification of metabolites by HPLC analysisand corecrystallizationTo verify the nature of the metabolites obtained from thetransformation of preg by human and porcine P450c17, weidentified by HPLC analysis,...
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Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
... Biochemistry 31 , 39 47 39 55.44 Record MT Jr, Ha JH & Fisher MA (1991) Analysis of equilibrium and kinetic measurements to determinethermodynamic origins of stability and specificity andmechanism of ... sys-tem was heated from 0 to 30 0 K at constant volume, andthis was followed by equilibration for 25 ps at a constanttemperature of 30 0 K and a pressure of 1 atm. The produc-tion phase was started ... solvent-accessible areas and DCpvalues is shown in Table 3. The SASA is reduced upon complexformation, and the majority of the reduction was dueto the burial of nonpolar surface. This is reflectedin...
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Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx
... 30 Gal(b1-O)pNP 38 44 30 37 34 Glc(a1-O)pNP 22 62 28 31 28Man(a1-O)pNP 29 205 22 59 29Fuc(a1-O)pNP 31 58 23 40 31 Xyl(a1-O)pNP 35 64 24 37 31 aAnti-FLAG-beads bound lysate of Sf9 cells.bThe D. melanogasterb1 -3 ... diglycosyl-ated peptide MUC5AC -3 ⁄ 13 and CG 139 04-1 wasmore active toward MUC5AC- 13 and MUC5AC -3 ⁄ 13 Table 1. Monosaccharide acceptor specificity of D. melanogastercore-1 b3GalT homologs.Acceptor ... Recombinantsoluble beta-1,4-galactosyltransferases expressed inSaccharomyces cerevisiae. Purification, characterizationand comparison with human enzyme. Eur J Biochem 239 , 34 0 34 8. 30 Ciucanu I...
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