Báo cáo khoa học: Afﬁnity puriﬁcation-mass spectrometry Powerful tools for the characterization of protein complexes ppt

... doi:10.1046/j.1432-1033.2003.03428.xAnalysis of protein complexes Within the scope of this review, we will focus on summari-zing the current technical state of the art for the biochemicalretrieval of associated proteins and protein complexes ... MINIREVIEW Affinity purification-mass spectrometry Powerful tools for the characterization of protein complexes Andreas Bauer and Bernhard KusterCellzome AG, Heidelberg, GermanyMulti -protein complexes ... excess of contaminating proteins (e.g. antibodies in IPexperiments).Conclusions The combination of affinity methods for the purification of protein complexes and the identification of their compo-nents...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... 1.6808 0.6612Y= the first right brother of the current node 0.4730 1.1525 0.7502Y= the first left brother of the current node 0.5832 2.1511 1.2186Y= the second right brother of the current node ... 0.5044 0.2525Y= the second left brother of the current node 0.0949 0.6171 0.2697Y= the first right brother of the parent 0.1068 0.3717 0.2133Y= the first left brother of the parent 0.2505 ... relation0.5832(Y= the first left brother)SD=1 1.1598(Y= the parent)0.4730(Y= the first right brother)0.2505(Y= the first left brother of the parent)0.0949(Y= the second left brother)SD=2 0.6483(Y= the...

Báo cáo khoa học: "COMMON TOPICS AND COHERENT SITUATIONS: INTERPRETING ELLIPSIS IN THE CONTEXT OF DISCOURSE INFERENCE" ppt

... retrieved by way of their corresponding node(s) in the syntax; the oper- ations performed on these representations are dictated by the nature of the constraints imposed. The two types of inference ... parallel to the overt con- stituents in the target are abstracted out of the clause representation. 4 For simplicity, we will assume that 3We will ignore the tense of the predicates for ease of exposition. ... constituent in the syntax, and (2) whether the form is anaphoric in the semantics. In subsequent sections, we show how the distinct mecha- nisms for recovering these types of missing information...

Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... mode of action for kCIII would be independent of the HflB substrate. Inthis study, we tested the ability of CIII to protect r32from HflB digestion. The inhibition of HflB-mediated proteolysis of ... in the pres-ence of CIII, and  65% of r32remained undigestedafter 80 min, compared with  40% for the control(r32alone, Fig. 2C). The inhibitory action of CIIIC on the proteolysis of r32by ... only for CII? Weexamined the mode of the inhibitory action of CIII onproteolysis of the heat shock sigma factor r32, anothersubstrate of HflB. Like proteolysis of CII, r32proteol-ysis...

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

... higher -affinity and lower -affinity binding sites are listed in Table 1. The high -affinity and the low -affinity sites accounted for 25% and 75% of the total binding, respectively.These estimates of the ... estimates of the number of binding sites from Scatchard plots difficult [22,23]. The higher -affinity and lower -affinity sites for PCSK9 binding on HepG2 cells may be indicative of either the presence of ... gain -of- function mutants of PCSK9 reduced binding of [125I]TC-PCSK9. The Scatchard plot of the binding-inhibition curve was curvilinear, indicative of high -affinity andlow -affinity sites for...

Báo cáo khoa học: Afﬁnity of S100A1 protein for calcium increases dramatically upon glutathionylation docx

... glutathionylatedsubunits of the S100A1 protein. Nevertheless, becauseformation of mixed disulfide between the subunits of E73Q and 2-mercaptoethanol does not affect the affin-ity of the protein N-loops for calcium, ... component. In the presence of 200 lmCa2+, the fluorescence of the protein peptide mixtureis reduced by 20% relative to the sum of the fluores-cence signals for TRTK-12 and the protein as meas-ured ... basallevel of  0.1 lm to  1 lm [25]. Therefore, inside acell, the isolated S100A1 protein should always remainin the apo state. Of course, the affinity of the protein for calcium can increase when...

Tài liệu Báo cáo khoa học: "A Feature Based Approach to Leveraging Context for Classifying Newsgroup Style Discussion Segments" pptx

... or other. For the two argumentation dimensions, the most natural application of sequential learning tech-niques is by defining the history of a span of text in terms of the sequence of spans ... span of text is to the spans of text in the parent message. This is computed using the mini-mum of all cosine distance measures between the vector representation of the span of text and that of ... done for text classification problems where very subtle distinctions must be made or where the size of spans of text being classified is relatively small. Both of these are true of our work. For...

... For this paper, we comple-mented the English dialogues of the corpus with aset of semantic annotations,1an example of whichis given in Figure 1. This example also exempli-ﬁes the type of ... derived from the Forwardalgorithm, of an observation sequence to inform the agent’s learning process.r =0 for reaching the goal state+1 for a desired ... otherwise.Whenever the agent has generated a word sequencew0 wn, the HMM assigns a reward correspondingto the likelihood of observing the sequence in the data. In addition, the agent is rewarded for shortinteractions...

Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35ﬁTyr mutant pdf

... chainconformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35fiTyr mutant from that of Phe35 of the wild-type cyt b5is 0.21 A˚, within the error limit. The sidechain of Tyr35 ... while the other one extends into the solventand does not form any hydrogen bond with the protein atoms. The former propionate displays the conservedconformation in the structures of the Phe35fiTyr ... mutantand of the wild-type protein as well as other mutants.However, the conformation of the latter is flexible.CD spectra of thermal denaturation of cytb5and its Phe35fiTyr mutantFig. 3A shows the...

Báo cáo khoa học: "Constituent-Based Morphological Parsing: A New Approach to the Problem of Word-Recognition" pdf

... as far as the phonology is concerned (i.e., they undergo Vowel Harmony 3 with the word to which they attach) are separate words from the point of view of the syntax. For instance, the auxiliary ... allowing for a bounded matching mechanism. 2.2 The Non.Isomorphism of Morphophonology and Morphosyntax Another fundamental property of morphology is the fact that the structure required for the ... Harmony 4, and, of course, reduplication. The input consists of the stream of segments and a stream of stressesS: pangupangu r nu 1 2 There is a question of course as to whether one could...

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