... Effects of various N-terminal addressing signals on sorting
and folding of mammalian CYP11A1 in yeast mitochondria
Irina E. Kovaleva, Lyudmila A. Novikova, ... confinement and folding in the inner mito-
chondrial membrane. In yeast mitochondria, only an
insignificant portion of the imported CYP11A1 follows this
mechanism.
Keywords: yeast mitocho...
... with increasing concen-
tration of TMAO was not due a reduction in the
binding affinity of ANS to FtsZ but due conforma-
tional change in the protein.
TMAO reversed denaturant-induced loss
of secondary ... decrease in one step with increasing
concentration of urea in the absence and presence of
TMAO. The D
m
values for the urea-induced unfolding
of FtsZ were found to be 1...
... monomer; in response to stress,
it forms homotrimers that bind to the HSEs in the
promoter regions of hsp genes [19]. In addition, an
in ammatory cytokine, interleukin-6, induces the exp-
ression of ... to the region between )206
and )187 bp of the hsp70 promoter in vitro and
in vivo, resulting in the induction of Hsp70 expression.
STAT3 is required for the induction...
... amplification of bovine genomic
DNA, using the following primer pairs: Met/C7 for intron
II amplification; N9/C6 for intron III amplification;
C8/C1 for intron IV amplification; C5/N10 for intron V
amplification. ... sequences encoding the two differ-
ent bovine tryptases (see Results).
Organization of bovine tryptase genes and location
of intron II–V
Intron II–V length of the two genes...
... central signal in a wide
variety of processes, including vasodilation, neural
communication and immune function in animals [1],
and defense responses, hormonal signaling and flower-
ing in plants [2–6]. ... compilation ª 2007 FEBS
Argininosuccinate is the immediate precursor to
arginine in the urea cycle, and is converted to arginine
and fumarate by argininosuccinate lyase...
... including
the N-terminal methionine. In the case of Ole e 8, a 550 bp
cDNA fragment corresponding to the coding region of a
protein of 170 amino acid residues including N-terminal
methionine was obtained ... rRNA staining with
ethidium bromide as an indication of the RNA loading
in each slot.
To confirm the expression of AtOle e 3 and AtOle e 8
proteins in the transgenic...
... response of the active fraction to a change of the
kinase concentration as a function of the concentra-
tions of the two forms ([T] and [T*]) and the elastici-
ties of the enzymes by the following ... Solid line: the case of kinase sequestration,
dotted line: no kinase sequestration. Grey lines indicate the stimu-
lus (i.e. kinase concentration), scaled by 0.1 in (A).
Effec...
... upon the substrate used, with
methylation of GST–GAR being more responsive to
inhibition than histone 4. A histone 4 and an arginine-
rich and glycine-rich peptide were recently compared in
kinetic ... activity and
cellular protein arginine methylation, including methyl-
ation of the guanine nucleotide exchange factor Vav1
[5]. Incubation with MDL 28,842 diminished methyla-
tion...
... results for a common query. Indexing
millions of search logs and listings can be done
in minutes. Reusing text search engines allows
a seamless integration between query parsing and
search.
We changed ... in a sin-
gle utterance
1
. Finally, many queries often con-
tain other constraints (assuming LocationTerm is a
constraint) such as that deliver in restaurants that
deliver or op...
... mutation consists of a single G fi A transi-
tion in codon 237, resulting in a missense mutation of
methionine to isoleucine [32,33]. Interestingly, a study
conducted by Van Meir et al. on different ... measured. Inhibition of the complex was
evident in both cell lines, but predominantly in U87 MG cells, and was
accompanied by accumulation of ubiquitin conjugates. Accumulat...