... Engineering, Graduate School of Engineering, Tohoku University, Miyagi, Japan The structure of membrane lipids is the most strikingcharacteristic of the Archaea (one of the three domains of ... the protein arose from the translation from Met at the 24th position of the expec-ted full-length of the archaeal enzyme (except for the polyhistidine-tag). In contrast, a distinct protein of slightly ... centrifuga-tion of the cell lysate, whereas the other two werefound in the precipitate. Therefore, in this study, weattempted to purify the recombinant protein encoded in AF0464 in order to understand...