... variants.However, their hyperactivity is suppressed by the pres-ence of the additional R764D deletion in War1-42p.Furthermore, we tested the strains expressing the mutant War1p variants for their ability ... 5A,War1-42p, like the wild-type control War1p, localizedto the nucleus in the steady state. As expected, Hxk1pwas predominantly found in the soluble cytoplasmicfraction. The signal for Hxk1p in the nuclear ... containing 5 mm sorbate and grown for another2 days. For YAK3, about 80 000 independent yeast colon-ies, and for YCS12Z-I about 50 000 colonies, were screened for the loss of sorbate-mediated lacZ...