... quantitation of microgram quantities of protein util-izing the principle of protein-dye binding. Anal Biochem72, 248–254.45 Newman LM & Wackett LP (1995) Purification andcharacterization of toluene ... residuesand the edge of the substrate ring could prevent bind-ing of 3,6-DMC and 3,5-DMC, as shown in Fig. 2-D,E,F. Thus, the binding of 3,5-DMC and 3,6-DMCto the active site of C2,3O could be ... of the active site changes with respect to theone observed in the crystal structure of C2,3O uponbinding of the dimethylcatechols.Since the CH atoms at position 5 of the substratering point...