Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc
... 2003
Tracking interactions that stabilize the dimer structure of starch
phosphorylase from
Corynebacterium callunae
Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed
mutagenesis
Richard ... structure.
The results have revealed clearly that Arg234 and Arg242 of
the TOWER interface region of StP partia...
... represents the impact of the piece of evidence
on the subsets of O. A bpa assigns a number in the range
[0,1] to each subset of O such that the numbers sum to 1.
The number assigned to the subset ... without cues. Furthermore, substantial improve-
ment is gained by the use of counters since they prevent
the effect of the "exceptions of the rules...
... in the K
i
by DS, and no effect on K
i
by Ch6S (Table 2). The
various effects of sulfated polysaccharides on inhibi-
tion of elastase-2 by eglin c and by the tetrapeptide
derived from his sequence ... in the absence or
presence of effectors, not to determine absolute values
from their dependence on the concentration of eglin c
and a
1
-PI. The effects...
... the steroid
output of the adrenal. The discovery that the two
CYP17 isoforms are two genes and that the genotypes
differ not only by the genes present, but also by the
copy number, suggests that ... to identify the distribution of the two CYP17 alleles
in the South African Angora population. These data revealed that the two
CYP17 isoforms were not th...
... indicate that the banded fibril
forming collagens are traversed specifically near their ÔdÕ
bands, within the gap region of the collagen fibrils, by the
filamentous beaded structures of the type ... without
the addition of complexes were performed. For the analysis
of interactions between proteoglycans and collagens, the
sensograms were corrected by a modificati...
... [4]. Other key attributes of
the catalytically competent structure are the ‘oxyanion
hole’ formed by the amide nitrogens of Ser195 and
Gly193, and substrate-binding subsites (S1, S2, S3, and
S4), ... chains of the catalytic triad residues (Asp102, His57, and Ser195) are indicated (yellow
for ‘open’; magenta for ‘closed’), as is that of the 220-loop residues 215...
... calculations
The netropsin amidinium end
The contact area of the Nt amidinium end (NAE) was
investigated by evaluating the interaction energies and
hydrogen positions of the end fragment and the bases
of ... of van der Waals’
interactions between the drug and the floor and walls
of the minor groove [21,22]. Electrostatic interactions
between the nega...
... by
cleavage between the methylthio-substituted carbons.
They clearly showed that the two species of monoeno-
ates formed by the partial hydrogenation of the
unknown fatty acid were 16:1 D-7 and ... acid methyl esters pre-
pared from the triacylglycerol fraction of the cells were analyzed by
GC. The number at the top of the peak on the chromatogram cor-...
... stopped by the addition of 2 mL of chloroform ⁄ meth-
anol (2 : 1, v ⁄ v) and 500 lL of water. The formed products
were extracted and analyzed as described above.
Statistical analysis
The level of ... in the
de novo synthesis of a given phospholipid, including
PI. Hence, the rate of synthesis and the amount of
CDP-DAG molecules that were used as substrate...
... likely that ubc9 and the UFD of
uba2 interact at the a1 region. (b) The region below
the a1 helix consists mainly of the N-terminal exten-
sion of a1 and loops between the b2 and b3 strands
and the ... E1 is composed of APPBP1 (APP
binding protein 1) and uba3. The domain structures of
Aos1 and APPBP1 resemble the N-termini, and those
of uba2 and...