Báo cáo khoa học: The GxxxG motif of the transmembrane domain of subunit e is involved in the dimerization/oligomerization of the yeast ATP synthase complex in the mitochondrial membrane doc
... enzyme.
Subunit e is involved in the oligomerization
of the yeast ATP synthase
An interesting result shown in Fig. 6 is the presence of
e + e dimers in the oligomeric forms of the wild-type ATP
synthase upon ... and
Saccharomyces cerevisiae (P81449, Swiss-Prot)
are in bold. The numbering of the yeast sub-
unit e begins at the initiat...
... Pharmacia
Biotech AB) at room temperature. Calcein entrapped in the
vesicles is highly concentrated, and the fluorescence is self-
quenched. Calcein release due to membrane permeation
induced by the peptide ... determine whether this process was affected by
the ionic strength of the incubation medium, we
assayed the peptide’s bactericidal effect, as defined in
Experiment...
... pretreated with TNFa followed by insulin for these time
periods. Control cells were incubated in the absence of any of
these agents. On termination of incubation, the glucose released in
the medium ... 2A–C). These results
imply that, in the presence of TNFa, wherein cells are
rendered insulin insensitive, insulin-mediated nuclear
exclusion and inactivation of Foxa2 ar...
... core of the pro-
tein in the N state and the evolution of the overall
structure of the protein in the MG state. This degree
of resolution is unprecedented for the T domain of the
diphtheria toxin. ... mono-
meric T domain and TH5 is protected because it is
involved in the dimer interface. TH9 can be considered
as involved in the protein core...
... little, if any, effect
on zinc release and that the predominant mechanism
of zinc release is the modification of the cysteine lig-
ands of zinc.
Aldehydes increase the concentration of
available cellular ... 4303
Aldehydes induce expression of metallothionein
in HepG2 cells
A cadmium-binding assay was used to examine the
expression levels of MT in HepG2 cells after...
... protein is the only protein synthesized
and labelled during the reaction. Consequently the iso-
tope-labelled amino acids are used very efficiently, and
because no new metabolic enzymes are expressed ... 3), as the increase in effective molecular
mass decreases the signal intensities in the [
15
N]-HSQC
spectrum [11].
Similarly, the presence of flexible polypeptide seg-
me...
... all these proteins [10,35]. In addition, the
sequence of PAC presented only one cysteine residue
that should be involved in the interchain disulfide
bridge.
The DNA fragment encoding PAC was inserted
into ... Alignment of the deduced amino acid sequences
shows that all residues involved in the active site as
described for abrin-a, abrin-c and ricin are conserved
in t...
... suspected that the linker between the
transmembrane domain and the tetramerization domain
may impair the co-operative effect on the assembly of
these two domains. Thus, new attempts were made to
Z. ... domains.
The different structural domains are
indicated by the bars above the protein
sequence. The linker between the KcsA
pore domain and GCN4 is underlined....
... sequence and the corresponding amino acids. Indicated for
the nucleotide sequence are the putative )10 and )35 promoter regions
(underlined), the ribosome-binding site (double-underlined), the ... (boldface) and the PstI site used for subcloning (dotted line).
Indicated in the amino acid sequence are the putative cleavage site of
the signal peptidase (arrow) and the am...
... mdi tren bffdc dffdng hien dai hoa
van
hpc.
Ve ke't ca'u, hau bet cac truyen ngan neu
tren deu cd ke't cau chat che, tinh tie't cau
chuyen dUa ra mdt
each
tff nhien (Chu ... chuyen deu d dang
md, lam cho ngUdi dpc phai suy nghT, nghiin
ngam ve nhan vat, ve eau chuyen, ve
the
thai
nhan tinh
Cd sU giang co, dien ra am tbam nhUng
khdng kem phan quye't liet...