... thatthis residue could affect the FAD environment, modulating the catalyticrate of the enzyme. Finaly, L315 affects the enzyme kcat, although it is notlocated in the near vicinity of the ... are situated at similar distancesfrom the hydroxyl of the docked substrate, they couldcooperate in facilitating the hydride transfer from sub-strate to FAD. The decrease of activity of the AAOH502A ... BX=Að2Þwhere A is the maximal turnover rate (kcat), X is the sub-strate concentration, K is the Michaelis constant (Km), andB is the catalytic efficiency (kcat⁄ Km). Mean and standarddeviations...