... chain;?, unknown cross-linking adducts;*, cross-linking adducts with E. coli L23;d, cross-linking adducts with E. coli Ffh;s, cross-linking adducts with B. subtilis Ffh;n, cross-linking ... protoplasts were used for SDS ⁄ PAGEand western blotting. In parallel, protoplasts were incuba-ted without trypsin, or in the presence of trypsin plus 1%Triton X-100.Cross-linking of in vitro synthesized ... that can insert into themembrane, helix-breaking glycine or proline residues areoften present in the middle of the hydrophobic stretch.Unlooping of this hairpin might result in the insertionof...