Báo cáo khoa học: Adenine and adenosine salvage pathways in erythrocytes and the role of S-adenosylhomocysteine hydrolase A theoretical study using elementary ﬂux modes Stefan Schuster and Dimitar Kenanov ppt

... Journal 272 (2005) 5278–5290 ª 2005 FEBS Adenine and adenosine salvage pathways in erythrocytes and the role of S-adenosylhomocysteine hydrolase A theoretical study using elementary ﬂux modes Stefan ... synthesis of ATP is possible. As for pathways using adenosine, a straightforward assumption is that these pathways startwith adenosine kinase. However, a pathway bypassing this enzyme and using ... is involved in these pathways. Since adenine is a substrate of ADPRT, the elevation of ATP in the absence of adenosine kinase shows that adenine must be released in the process before being incorporated...

Báo cáo khoa học: Modular kinetic analysis reveals differences in Cd2+ and Cu2+ ion-induced impairment of oxidative phosphorylation in liver pot

... suspended in the medium containing 250 mmsucrose and 5 mm Tris (pH 7.3). Protein was estimatedaccording to Bradford [39] using BSA as the standard.Measurement of respiration and membranepotentialPrior ... [29,30].Bimodulular kinetic analysis of the effects of Cd2+ and Cu2+ions on oxidative phosphorylation The data in Figs 2 and 3 indicate that Cd2+ and Cu2+ions affected the respiratory chain module. Therefore,as ... (C)quantifies the importance of each enzyme (module) in the pathway in controlling the flux through that pathway at a steady state. It represents the fractional change in the system property (flux, ...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... evaluation modelfor statistical syntactic parsingGiven a sentence, the task of statistical syntacticparsing is to assign a probability to eachcandidate parsing tree that conforms to the grammar ... Predictive information redundancy can beused as a measure of the redundancy between the predictive information of a feature type and that of a feature type combination. Predictive Information Summation ... ConclusionAmong the feature types in the same structuralposition of the parsing tree, the predictiveinformation quantity of lexical feature type islarger than that of part -of- speech feature type, and the...

Báo cáo khoa học: Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment pptx

... evaluated on a long-lasting and functional basis, rather than on an acute and his-tological basis. Also, careful and rigorous selection of patients with salvageable tissue [evidenced using mag-netic ... ItalyTherapeutic strategies aimed at reducing brain dam-age after ischemic stroke have been a major focus of academic and industrial research for the past30 years. Two primary therapeutic approaches ... release of ROS from NADPH oxidase and mitochondria, sus-tained increase of the cytosolic Ca2+concentration and ﬁnally nuclear translocation of mitogen-activatedprotein kinase kinase ⁄ extracellular...

Báo cáo khoa học: Ferrous Campylobacter jejuni truncated hemoglobin P displays an extremely high reactivity for cyanide – a comparative study ppt

... [30,36,41,42]. In contrast, the excellentTable 1. Values of kinetic and thermodynamic parameters for cyanide binding to ferric and ferrous heme-proteins. Values in italic were cal-culated according to the ... displays the highest affinity as well as the fastest combination and the slowest dissociation rate for cyanide binding of the known members of the Hb superfamily. Furthermore,as the kinetics of ... typecomprises flavohemoglobins (flavoHbs), which arecharacterized by the classic 3-on-3 a- helical sandwichglobin domain, hosting the heme, covalently linkedto a flavin reductase domain. The second...

Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx

... FEBSexecuting the terminal stage of a myriad of signalingcascades that initiate at the cell surface and reach theirclimax in the nucleus [8–10].Upon activation by mitogen-activated protein kinas-es, ... domains containing Trx-tag at the N-terminus and His-tag at the C-terminus. Addition-ally, titration of a protein construct containing thioredoxinwith a C-terminal His-tag (Trx-His) in the calorimetric ... change in thermal power as a function of each injection was automatically recorded using origin software and the raw data were further processed toyield binding isotherms of heat release per injection...

Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... through the interactions of the DnaK–DnaJ–GrpE machinery and by HﬂB-mediateddegradation. Interestingly, these latter proteins thatpromote the degradation of r32are themselves pro-duced as a result ... binding, 5% of the inputsolution was kept aside separately and was used as a controlfor comparison. The proteins were analyzed in an 11%SDS ⁄ PAGE and by western blotting using anti-his Ig. As ... min. The amount of r32thatremained after proteolysis was analyzed after SDS ⁄ PAGE of the samples followed by Coomassie Brilliant Blue stain-ing and quantitation in a gel documentation system...

Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc

... Panel on Euthanasia of the AVMA and in compliance with the CINVESTAV and Trudeau InstituteIACUC guidelines. The IL-3 dependent murine pro-B cellline, Ba/F3 (a generous gift from D. Campana, ... °C. The cells were analyzed by cytometry using a FACSCalibur (Becton-Dickinson, San Jose, CA, USA).Surface biotinylation of proteinsTo analyze the stability of CD38 on the plasma membrane of the ... homologues, the cytosolic Aplysia califor-nica ADP-ribosyl cyclase [11,12] and the mammalianGPI-anchored NAD+glycohydrolase, CD157 [13,14].Crystallographic and X-ray diffraction analyses of thesetwo...

Báo cáo khoa học: Adenine, a hairpin ribozyme cofactor – high-pressure and competition studies potx

... self-cleavage kinetics of ADHR1 are independent of the order of addition of the cofactors adenine and Mg2+. The kinetics of self-cleavage of ADHR1 were analyzed after 10 min of preincu-bation ... showed that the specificity of adenine in restoring the catalytic activity of ADHR1 is ratherloose [16], and that some adenine analogs, such as6-methyladenine, purine, and even imidazole, can alsoconfer ... significantly more efficient than adenine in restor-ing the catalytic activity. In an attempt to obtain addi-tional information about the binding of adenine and some of its analogs to ADHR1, competition...

Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

... nuclear factor kappaBactivity and are independent of the B-Raf ⁄ mitogen-acti-vated ⁄ extracellular signal-regulated protein kinase path-way and the Akt pathway. Cancer 104, 879–890.26 Takada ... MAPKsignaling pathways of p38 MAPK and ERK1⁄ 2. The simultaneous activation of both MAPK pathways isindicated by the similar time dependence in the activa-tion of ERK1 ⁄ 2 and p38 MAPK. Both pathways ... further investigated whether the activation of ERK1 ⁄ 2 and p38 MAPK may be part of the antipro-liferative activity of ATP, as these kinases are import-ant regulators of proliferation as well as apoptosis.Western...

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