Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes docx
Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes docx
... Biochem. 270) 4605 Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes Francesco Milano1, ... that buries the quinone head in the inner part of the QBpocket, thereby increasing the interaction energy between the ligand and the binding site. In a forthcoming work the exchange kinetic dependence ... data[14] shows that the detergent itself is located in the channelinto which the quinone isoprenoid chain sits in the enzyme.This explains the slow exchange process of the quinone at itsbinding...
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Báo cáo khoa học: Kinetics of the inhibition of neutrophil proteinases by recombinant elafin and pre-elafin (trappin-2) expressed in Pichia pastoris ppt
... properties of bound trappin-2, as this covalentlinking may increase significantly the bioavailability of such an inhibitor at the site of in ammation, e.g. in the case of therapeutic administration, ... contribute to maintainingan in ammatory state and favor the persistence of microbialinfections. Taken together, these observations suggest thatincreasing serine protease inhibitor levels in lungs, ... about95% as inferred from active site titration experiments anddetermination of the protein content by the Bradford method.Determination of the equilibrium dissociation constant Kifor the interaction...
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Báo cáo khoa học: Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of b-lactam antibiotics pptx
... may bind in the acyl- binding site of PA. Hydrolysis of p-NPA may thus proceedvia reversed binding of the substrate, in which the leavinggroup, p-nitrophenol, occupies the acyl binding site ... kinetic constants forhydrolysis and synthesis of penicillin G, ampicillin andcephalexin. Furthermore, insight into the nature of the transition state in the rate-determining steps of the reaction was ... Vs/Vhratio is the ratio of the reaction rates of aminolysis and hydrolysis of the acyl-enzyme. The curva-ture of the plot of Vs/Vhvs. the mole fraction of D2Oindicates therefore that the deuterium...
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Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
... essentially independent of pH in the range 5.0–8.0 (not shown).Reduction of the rubredoxin domain of FlRdby flavorubredoxin reductase The isolated, genetically truncated rubredoxin domain(Rd-domain) of ... herein investigated by time-resolvedspectroscopy working on the purified recombinantproteins.ResultsReduction of flavorubredoxin reductase by NADH The kinetics of the reduction of flavorubredoxin ... configuration,bringing the FMN of one monomer in close proximityto the Fe-Fe site of the other monomer. It is thereforelikely, though not proven yet, that the dimer is the functional unit of this...
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Báo cáo khoa học: Kinetics of inhibition of acetylcholinesterase in the presence of acetonitrile doc
... with the parameter estimatesobtained in the absence of the inhibitors (see above). In the case of gallamine investigated in the presence of MeCN, the calculation of the kinetic parameters wasbased ... after incubating the enzyme–inhibitor mixture for 15 min at 25 °C. Uninhib-ited enzyme activity was determined by adding the corre-sponding solvent instead of the inhibitor solution. The rates of ... AChE-catalysed ATCh cleavage were corrected by those of the nonenzymatic hydrolysis of ATCh obtained in the absence of enzyme and inhibitor. The rate of the AChE-catalysed hydrolysis of 500 lm ATCh...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... the multiple cloning site of pUC18 at their N-terminal ends. The black bars in the GBd indicate the ‘A’ repeat [6,7]. Kinetics of Streptococcus sobrinus GTF-I reaction H. Komatsu et al.532 FEBS ... in the intermediate oligosaccharides during the glucan syn-thesis can be interpreted with respect to the kineticproperties of enzymes. In other words, the relationshipbetween the enzyme kinetic ... supports the appropriateness of the model in Scheme 1; thus, ourresults provide a reasonable explanation for the dex-tran-independent glucan synthesis reaction. At the beginning of the reaction, ...
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Tài liệu Báo cáo khoa học: High-affinity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx
... binding. Pairing of wild-type and point-mutated IGF-II binding sitesbetween two dimerized mini-receptors had no effect on the function of the contralateral binding site, indicating IGF-II binding ... Man-6-P binding or whether the mutated binding pocket becomes distorted, preventing Man-6-P binding. Glucose 6-phosphate (Glc-6-P) is unable tobind to the two Man-6-P binding sites of the receptor[17]. ... IGF-II binding must only be a localeffect because binding of PMP-BSA, which binds toother sites in the ectodomain of these heterodimers,resulted in the predicted amount of ligand binding. This...
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Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt
... investigated the metal -binding reactivity of the isolated a domain of human metallothionein isoform 1a, with specificemphasis on resolving the debate concerning the cooperative nature of the metal -binding ... example of the complexity introduced by the presence of the bdomain in efforts to elucidate the potentially cooper-ative nature of the metal -binding reaction within each of the domains. Thus, the ... to elucidate the meta-llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation details of the two-domain protein. The results...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... formpart of the copper binding sites in this protein.Although there are four histidines in the C-terminal,another histidine is present at position 18, and given the folded state of the protein it ... among the three human cystatins.Further support for the premise that the C-terminuscould be the copper binding domain comes from stud-ies of the P79S mutant, which differs from the copper binding ... affinitycopper binding protein, and (b) that amyloid fibrilformation of this protein is diminished in presence of Cu2+ions. Another interesting finding is thatcopper binding gets less strong at pH 5,...
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Tài liệu Báo cáo khoa học: A novel c-N-methylaminobutyrate demethylating oxidase involved in catabolism of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1 ppt
... c-N-methylam-inobutyrate, a compound generated during the catabolism of nicotine. The identification of this enzyme reveals, for the first time, the metabolic fate of the pyrrolidine ring of nicotine during ... familymay indicate that this is the site of attachment of tetra-hydrofolate to the enzyme. c-Aminobutyrate producedduring the reaction may enter the general metabolism.Compared to kinetic data from ... 0.51. The redanionic flavin semiquinone was formed for more than 99%during the reaction, indicating that the redox potentials of the two couples (oxidized/semiquinone and semiquinone/hydroquinone)...
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