Báo cáo khoa học: Disruption of transport activity in a D93H mutant thiamine transporter 1, from a Rogers Syndrome family pdf
Báo cáo khoa học: Disruption of transport activity in a D93H mutant thiamine transporter 1, from a Rogers Syndrome family pdf
... Disruption of transport activity in a D93H mutant thiamine transporter 1, from a Rogers Syndrome family Dana Baron1, Yehuda G. Assaraf2, Stavit Drori2and Ami Aronheim11Department of ... withthe D93H mutant THTR1 and treated with tunicamycindisplayed a similar lack of plasma membrane localization of the mutant transporter as well as a typical subcellularlocalization that was confined ... that are aimed at characterization of the impactthat these missense THTR1 mutations have on the expres-sion, post-translational modification, plasma membranetargeting and thiamine transport activity. ...
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Báo cáo khoa học: Loss of kinase activity in Mycobacterium tuberculosis multidomain protein Rv1364c pot
... orthovanadate,calyculin A, cyclosporine, and okadaic acid, was stud-ied. Insensitivity to okadaic acid, a potent inhibitor of the PP 2A and PP2B family of phosphatases, is one of the unique characteristics ... compilation ª 2008 FEBSLoss of kinase activity in Mycobacterium tuberculosismultidomain protein Rv1364cPreeti Sachdeva 1,2 , Azeet Narayan1, Richa Misra1, Vani Brahmachari2and Yogendra Singh11 ... proteins. In view of conservation of specific interactions with cognate sigma andanti-anti-sigma factor, the loss of kinase activity of Rv1364c and UsfXappears to form a missing link in the phosphorylation-dependent...
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Báo cáo khóa học: Disruption of the interaction between the Rieske iron–sulfur protein and cytochrome b in the yeast bc1 complex owing to a human disease-associated mutation within cytochrome b potx
... below.Instability of the mutant enzymeFurther analysis of the kinetic parameters of the G167E mutant was hindered by rapid inhibition of the activity of the bc1complex during the assay, which was particularlynoticeable ... the activity in samples from the patient.This was in agreement with the data obtained in this study,using the mutant yeast enzyme. It has been reported that a mutation in the hinge region of ... mitochondrial membranepreparations (data not shown). This could be a result of the instability of the mutant enzyme and an increasedsensitivity to degradation during membrane preparation,as discussed...
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Báo cáo khoa học: Connection of transport and sensing by UhpC, the sensor for external glucose-6-phosphate in Escherichia coli ppt
... Germany).The radioactivity was quantified in a Canberra-PackardTricarb-2500 counter. The kinetic constants of transport were estimated using the method of Hanes. All datarepresent means of at least three ... butrecent analysis demonstrated that UhpC from E. coli canact not only as a sensor but also as a carrier that facilitates a Glc6P/Piantiport mode of transport [8]. The transport activity of UhpC from ... suggesting that Glc6Pcan be transported by a molecule locked in the inducingconformation. Introduction of an intrahelical salt bridgeincreased the transport activity of UhpC but abolishedsensing....
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Báo cáo khoa học: Disruption of the gene encoding 3b-hydroxysterol D14-reductase (Tm7sf2) in mice does not impair cholesterol biosynthesis pdf
... 5¢-TGCTCTCCTACTCCTGATGAAG-3¢; reverse, 5¢-GGGCATGTGGTTCCTGTCC-3¢. Cdc20: forward, 5¢-GCAACAGGAGGAGGAACCAG-3¢; reverse, 5¢-CATCCACAGCACTCAGACAGG-3¢. Cdkn 1a: forward, 5¢-AAAGTGTGCCGTTGTCTC-3¢;reverse, 5¢-AAAGTTCCACCGTTCTCG-3¢. ... 5¢-AAAGTTCCACCGTTCTCG-3¢. Accn5: for-ward, 5¢-GGTGACCATCCGCCAACTG-3¢; reverse, 5¢-CCGTAAGTGCTGTAGGTAATGAAG-3¢. Onecut1: forward,5¢-CCTCTATGAATAACCTCTATACC-3¢; reverse, 5¢-TGCTGGGAGTTGTGAATG-3¢. A. ... substrate. Activity was measured on the basis of C27D8forma-tion and C27D8,14disappearance, evaluated as the peakarea ratio between the individual sterol and cholestane,the internal standard.No...
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Báo cáo khoa học: Disruption of structural and functional integrity of a2-macroglobulin by cathepsin E pptx
... chromatography as des-cribed previously [28]. Antiserum against bovine a2 M waspurchased from Yagai Research Center (Yamagata, Japan).All other chemicals were of reagent grade and werepurchased from various ... hydrolyzing activity of cathepsin E and cathepsin DTo assess the inhibitory capacity of a2 M from varioussources for cathepsin D and cathepsin E, we first purifiedbovine a2 M. The final preparation gave ... 3.8within a 2-min incubation (Fig. 7B). Parallel to this change, a significant amount of the original a2 M disappeared and a Table 1. The ability of cathepsin E and cathepsin D to cleave synthetic...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... expression and activity of CYP2E1 weredownregulated in a rat hepatoma cell line after theadministration of proinflammatory cytokines, leadingto a loss of catalytic activity. This downregulation wasat ... significantly at24 h after CLP and this increase was attenuated byGdCl3. The malondialdehyde (MDA) level in CLP ratswas 1.8-fold that of sham-operated rats. The increase in the MDA level at 24 ... response.AbbreviationsALT, alanine aminotrasferase; AST, aspartate aminotrasferase; CLP, cecal ligation and puncture; CYP, cytochrome P450; GdCl3, gadoliniumchloride; GSH, glutathione; GSSG, glutathione...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... Horse-radish peroxidase-conjugated antibody against caspase-3(#610325) was from BD Pharmigen, and antibody againstb-actin (A5 441) was from Sigma.Statistical analysisAll data are expressed as ... Asshown in Fig. 4A, increased phosphorylation of eukaryotic translation initiation factor 2a (eIF 2a) wasapparent after 16 h of stearate treatment, reached a peak at 22 h, and declined thereafter. ... examinedthe main steps involved, following the uptake of satu-rated and unsaturated FFAs into the cells. After theirinternalization, FFAs are converted to fatty acyl-CoA, a reaction catalyzed...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... forward 5¢-GTAGGCATGAGAACGGGAAG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGAAA-3¢ and for CERK-negative forward 5¢-CCGCAAGAGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGACACGGAGAT-3¢, as a negative control ... MEBCYTO Apoptosis Kit waspurchased from Medical and Biological Laboratories(Nagoya, Japan), and a Nuclear Extract Kit was from Active Motif (Carlsbad, CA, USA). The ChIP Assay Kitwas also a product ... ichthyosis, mutations have been identified in theABCA12 gene, which encodes a member of the lipid transporter ATP-binding cassette (ABC) family that isalso part of the ABCA subfamily. ABCA12 is thoughtto...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... El-Agnaf OM & Allsop D(2002) Formation of hydrogen peroxide and hydroxylradicals from A( beta) and alpha-synuclein as a possiblemechanism of cell death in Alzheimer’s disease and Par-kinson’s ... FEBSdownstream of the initiator ATG in exon 1 (Ex1) of the 67 exon-containing htt gene [5]. Pathogenesis in HD correlates with the cleavage of mutated htt andthe release of an N-terminal fragment bearing ... toxic andprecipitate as insoluble fibers in affected neurons [8]. In human and HD transgenic mice, the disease corre-lates with the appearance of intraneuronal, intranu-clear and perinuclear aggregates...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ