Báo cáo khoa học: The light-harvesting antenna of the diatom Phaeodactylum tricornutum Evidence for a diadinoxanthin-binding subcomplex doc

Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt

... supported by the Natural Sciences andEngineering Research Council (NSERC) of Canada(RF: OGPO157551), by the Canadian Diabetes Associ-ation (CDA) and a grant from the Fondation pour laRecherche ... that the luminaldomain of DPP IV carries dominant apical sortinginformation while the short cytoplasmic tail and the transmembrane domain contain competing basolateralsorting information [17]. ... (La Jolla, CA). Reagents for SDS ⁄ PAGE were obtained from Bio-Rad (Mississauga,ON). bpV(phen) was synthesized as described [61]. Allother chemicals were of analytical grade and were pur-chased...

Báo cáo khoa học: Genome-wide analysis of clustering patterns and ﬂanking characteristics for plant microRNA genes doc

... larger) than the average distances of miRNApairs to evaluate the statistical signiﬁcance for clusteringpatterns in the four plant species.Prediction of the core promoter of the plantmiRNA ... of known miRNAs are arranged in clusters. For example,48% of human miRNAs appear as clusters within a maximum inter-miRNA distance of 10 kb [21] and50% of miRNAs appear as clusters within a ... of China, the 863Program (grant nos 2007AA02Z329), the NationalBasic Research Program of China, the 973 Program(grant nos 2008CB517302) and the National ScienceFoundation of Heilongjiang Province...

Báo cáo khoa học: The light-harvesting antenna of the diatom Phaeodactylum tricornutum Evidence for a diadinoxanthin-binding subcomplex doc

... diatoms, DD and DT aremainly associated with the FCP antenna [12], but theirexact localization in the different subfractions of the antenna has not yet been determined.Therefore, the aim of ... differs inmany aspects from that of green plants and algae.There are no grana stacking and no segregation of the photosystems [18]. The main components of the antenna are the fucoxanthin chlorophyll ... in diatom LHC G. Guglielmi et al.4348 FEBS Journal 272 (2005) 4339–4348 ª 2005 FEBS The light-harvesting antenna of the diatom Phaeodactylum tricornutum Evidence for a diadinoxanthin-binding subcomplex Ge´rard...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... possibly of other Meis paralogs, may play a role in regulating transcriptional activity, but itappears that the Hth domain does not maintain the cytoplasmic localization of Prep1. Additionally, the greatest ... ifinteraction of Pbx1 with the Hth domain altered the conformation or intramolecular interactions, allowingaccess to the AD. As the autoinhibition affected anunrelated AD when this was put in place ... proteins, and the (Gal)5-TATA lucifer-ase reporter (A) , or the (Gal)5-SV40 reporter (B). Luciferase activity was assayed after 48 h, and is presented as the mean + standard devia-tion of duplicate...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... the globaloptimum.As there are many random steps involved, the algorithmcan traverse the multidimensional parameter landscape inan inﬁnite number of ways, at least in a practical sense. The ... property and size of anamino acid residue could affect the protein negatively. The novel variables, the calculated energy for a spe-ciﬁc residue exchange and for the average of all aminoacid substitutions ... of information regarding the activity or loss of activity of the target protein, as only a few variants (< 100)have been fully analysed. In this regard, analysis of the p53 gene can be a paradigm...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... GCA CAC TAT ATT AAA CGG CAA AGC CGTAAA ACC CCG TGT AGG CTG GAG CTG CTT CG-3¢) and rluD114::cat(pKD3) 3¢ (5¢-GAC CAG ATT AATGTG AAA AGA AAA TCA CGC GTA CCG GAT CGTCTT GAT GGG AAT TAG CCA ... to the RluA family [2]. Binding of RluA to one of its substrates, tRNAPheanticodonstem-loop, induces reorganization of the RNA [21]. Anability of the RNA substrate to adopt the alternativefold ... CCT GGCCAT CAT TAC GCC) and AMV reverse transcriptase(Seikagaku Corp., Tokyo, Japan) in the presence of [a- 32P]dCTP (Amersham Biosciences, Piscataway, NJ,USA). The resulting DNA fragments...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... molecule, and individual mutations drasticallyreduced the activity. Because Ala lacks the character-istic side chains of Glu and Arg, the mutant recep-tors are devoid of the functional groups at the particular ... lM) for the basal constitutive activity, and the inverse antagonist activity of BPA (1 and10 lM) against the inverse agonist activity of 4-OHT (1 and 10 lM). The assay set marked with an asterisk ... activ-ity of 4-OHT and the inverse antagonist activity of BPA are observed for both (275Ala)-ERRc and(316Ala)-ERRc mutant receptors, and even for (Ala,Ala)-ERRc.DiscussionDifferential capacity...

Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt

... pep-tide variants of IA3; and to Doug Lamont and KennyBeattie, University of Dundee, for carrying out mul-tiple mass spectrometry analyses of the IA3variants. The endless patience, tolerance and ... inhibitors of aspartic proteinases arerather rare in nature. Thus, there is a need to under-stand the mechanisms of action of the few that areknown, in order to exploit their therapeutic potential[1]. ... related to that of sac-charopepsin (e.g. the vacuolar aspartic proteinase fromP. pastoris; PpPr) [1]. This shares a sequence identity of 77% with saccharopepsin and essentially all of the active...

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... located at the interface between the N-terminal and the C-terminal a- helices, indicatedFig. 5. Thermal stability of the A- state of cytochrome c as a func-tion of sulfate concentration. Sulfate ... the sulfate-induced destabilization of the HSacid-denatured form, with further formation of the LS(IHH) A- state; (b) for the slow phase, to formation of the LS native-like (IHM) A- state. Unlike ... 2006)doi:10.1111/j.1742-4658.2006.05527.xStructural information on partially folded forms is important for a deeperunderstanding of the folding mechanism(s) and the factors affecting proteinstabilization. The non-native compact state of...

Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-ﬁn eelpout, Zoarces elongatus Kner ppt

... mm of nfeAFP8.These data indicate that ‘less active’ AFP isoforms canexert a substantial level of antifreeze activity after the addition of a small amount of ‘active’ isoform. The less active ... clearconcentration dependence on, the addition of a smallamount of a QAE1 isoform, nfeAFP8 (Fig. 7). This issimilar to the case of the QAE2 isoform, nfeAFP13; the activity of its monomer was enhanced ... isoformbecomes ‘active’ when a small amount of QAE1 is added. In contrast, itdoes not become active on the addition of another SP isoform. Theseresults suggest that the SP and QAE isoforms of...

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