... Five minutes after the samplehad been injected, the concentration of ammonium acetate,pH 9.0, was increased linearly to 0.2 m in the first 20 min,Fig. 6. Sugar component analysis of pN4. The ... weretagged with the fluorophore 2-aminopyridine. Lyophilizedsamples were heated at 90 °C for 60 min with 20 lL of pyr-idylamination reagent, and then heated at 80 °C for 35 minafter the addition of ... hypothesis, it isnecessary to compare the genes involved in the synthe-sis of N-glycans in many organisms. The hypothesiswould gain strong support if lower metazoans werefound to have the...