... Nuclear import of murine PER and CRY proteins in Xenopus oocytes. mPER1 and mPER2, but not mPER3, are phosphorylated and
imported into the nucleus of Xenopus oocytes. (A)
35
S-labelled mPER1, ... the C-terminal
region of mPER1.
Complex formation with mPER1 promotes
nuclear import of mPER3 in Xenopus oocytes
As heterodimerization of clock pr...
... decreases in intracellular spermine pools
and increased spermidine levels in SMO1 and SMO5
cells. These changes in spermine and spermidine levels
in both SMO1 and SMO5 overexpressing cell lines
were ... microscopy of
immunofluorescent staining of SMO in cells overex-
pressing each of the isoforms provided direct visuali-
zation and confirmation of protein localiza...
... upon
incubation with increasing concentrations of single
polyamines, starting from a concentration of 1 lm
polyamines, which is comparable to the concentration
of polyamines forming NAPs in the ... associated with a
reduction in the overstretching forces of the DNA
strands [22,23], (b) that the DNA melting tempera-
ture is directly correlated with the chain length of
inter...
... B and A chains of insulin [1] and
has an important structural role in the proper folding
and disulfide bonding in insulin [2]. After proinsulin
cleavage, it is released to the blood together with ... process.
Early studies on proinsulin and insulin behavior in
solution revealed self-associating properties [19–21]
and, as a result, insulin is found to form zinc-induced
hexame...
... is
conserved in all subunit types (Hc1 and Hc2) with the
exception of PmaHc1. An insertion of nine amino
acids in PamHc2 starting at amino acid 435 is unique
to subunit types 2. On the amino acid ... coordinated by
three histidines in two distinct binding sites. Some
hemocyanins assemble into large oligomers of up to
8 · 6 subunits [1]. The occurrence and properties of
hem...
... to
prevent the entry of bacteria into the epithelial layer
and their invasion into the lower parts of the gastro-
intestinal tract [7]. Their finding of buforin-I in
porcine, bovine and human stomach ... membrane
and binding to the DNA without inducing cell lysis. In
the present study, HNr and H4-(86–100), like the
quinolone antibiotic ciprofloxacin, displayed rapid
(within 30...
... Localization of silicatein in spicules
and in intra- and extracellular vesicles by
TEM immunogold labeling. (i) Association of
silicatein with spicules. (A) Strong antibody
reactions are seen within the ... bonds (red). In the cata-
lytic center of silicatein, the serine (Ser) oxygen makes a nucleo-
philic attack on the silicon, resulting in displacement of
p-aminophenol...
... 2008)
doi:10.1111/j.1742-4658.2008.06522.x
The folding of eukaryotic DNA into the 30 nm fibre comprises the first
level of transcriptionally dormant chromatin. Understanding its structure
and the processes of its folding and unfolding is ... or of nucleosomes in the fibre [5,19]. It
is a cleavable crosslinker with two succinimidyl groups,
which react with lysines independently....
... Treatment with Quercetin
did not inhibit ROS generation, and enhanced cytotoxicity of
Topotecan in both cells. In contrast, NAC enhanced resistance
against doxorubicine and vincristine in MRP1 ... T-helper cells, and 50% of the young Ung–/– mice
investigated have no detectable NK/NKT-cell population in their
spleen. In addition, production of the cytokines interferon...
... phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2
confers high-affinity and bifunctional binding to both the SH2 domain
of Grb4 and the PDZ domain of the PDZ-RGS3 protein
Zhengding Su, Ping Xu and ... effect of tyrosine phosphorylations on the binding affinity
of ephrinB2(301–333) to the SH2 domain of Grb4 and to the PDZ
domain of PDZ-RGS3. In the combinat...