Báo cáo khoa học: Formation of nucleoprotein RecA ﬁlament on single-stranded DNA Analysis by stepwise increase in ligand complexity potx

... 2745 Formation of nucleoprotein RecA filament on single-stranded DNA Analysis by stepwise increase in ligand complexity Irina P. Bugreeva, Dmitry V. Bugreev and Georgy A. NevinskyInstitute of Chemical ... thandsDNA. After strand exchange, the second RecA DNA binding site binds the displaced strand followingnew DNA duplex formation [12].Binding of dsDNA to a RecA filament is followed by search of ... filament. ResultsFilamentation of RecA on ssDNAand its inhibition In the presence of ATP or ATPcS RecA is polymer-ized on DNA forming a nucleoprotein filament. Wehave studied the stability of a RecA filament formedwith...

Báo cáo khoa học: Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin pptx

... M (2004)Kinetics and binding sites for interaction of the prefol-din with a group II chaperonin: contiguous non-nativesubstrate and chaperonin binding sites in the archaealprefoldin. J Biol ... Chauhan VP (1998) Binding of amyloid beta-proteinto intracellular brain proteins in rat and human. Neuro-chem Res 23, 1277–1282. Formation of amyloid beta oligomers by prefoldin M. Sakono et al.5992 ... humanbrain [42], our results suggest a possible involvement of PFD in the formation of toxic Ab oligomers in thecytosol.ResultsFibrillation of Ab peptide in the presence of PFD In an effort to investigate...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... glucansynthesis.AbbreviationsDP, degree of polymerization; GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrasedomain; GSGB, glucosyltransferase-I containing a ... of dextran-independent insoluble glucan formation andexamined the enzyme kinetic properties of the nigero-oligosaccharide acceptor reaction by using GBd-deficientGTF-I (GS) and GTF-I containing ... Fig. 3).Finally, the concentration of insoluble glucan increasedwith a lag time of 60 min (filled squares in Fig. 3). Theglucosidic linkages of the insoluble glucan produced by GS or GSGB were...

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... expression of any gene of interest. Gene silencing by RNA interference (RNAi)is mediated by endogenous proteins, resulting in tar-get mRNA cleavage or translational inhibition [1]. In the cytoplasm ... compilation ª 2009 FEBS 6579 of TRBP in this complex. Binding reactions performed in extracts after TRBP silencing showed a concomitantreduction in binding at the expected location (Fig. S3).Based on ... prominent than that of Drosophila Dicer-2, which is controlled by R2D2binding rather than actively participating in determiningwhich end to bind [18]. Future work examining internaland terminal...

Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... that the conformation-al changes of C-LytA upon binding of these alkaloids are different to thoseinduced by choline, suggesting a different mode of binding. In vitro inhibi-tion assays of three ... cultures to increasing concentrations of cho-line abolishes daughter cell separation at the end of cell division, causing the formation of long chains of cells with only small effects on cell growth ... ligand binding to C-LytA. (A) Con-formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands:s, d, 2.5 and 20 mM choline; n,...

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

... critical r ole in the regulation of cellproliferation, including SMC, by inhibiting signalling mol-ecules [ 45–47]. Caveolin-3 is the main isoform in musclecells, including SMC [30]. Caveolin-1 and ... of MAPK [12,43,44], the inhibition of MAPK by the incorporation of n-3 PUFAs could alterthe phosphorylation of these transcription factors, affectingtheir binding to DNA and trans-activation. ... in untreated cells (Fig. 1A).However, the incorporation of BrdU into SMC wasstrongly stimulated by incubation in medium containing10% ( v/v) fetal bovine s erum. A lthough the incorporationof...

Tài liệu Báo cáo khoa học: Cleavage of nonphenolic b-1 diarylpropane lignin model dimers by manganese peroxidase from Phanerochaete chrysosporium Evidence for a hydrogen abstraction mechanism docx

... was conducted in the absence of Tween 80. In contrastto the oxidation of this substrate by lignin peroxidase (LiP),oxidation of substrates by MnP did not proceed underanaerobic conditions. ... (polyoxyethylenesorbitan monooleate)(0.1%). LiP reactions were conducted at 28 °Cfor5min in 1 mL of 20 mMsuccinate, pH 3.0, containing thesubstrate (180 lM) and enzyme (5 lg). The reactions wereinitiated by ... aremainly responsible for the initial depolymerization of lignin in wood [2,3,22,41]. While both enzymes are iron heme-containing peroxidases, their detailed reaction mechanismsdiffer considerably....

Báo cáo khoa học: Suppression of a cold-sensitive mutant initiation factor 1 by alterations in the 23S rRNA maturation region pdf

... mainphases – initiation, elongation, termination, and recy-cling. Initiation is the rate-limiting step [1]. This phaseis mediated by initiation factor 1 (IF1), initiation fac-tor 2, and initiation ... Translation initiation regiondependency of translation initiation in Escherichia coli by IF1 and kasugamycin. FEBS J 277, 2428–2439.12 Milon P, Konevega AL, Gualerzi CO & Rodnina MV(2008) Kinetic ... factor 3, determines the rates of ribo-somal subunit joining by inducing conformationalchanges in the 30S subunit [12].Each of the seven rRNA operons in E. coli is tran-scribed as a single primary...

Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx

... C-terminal domain of Ca2+-free CaM,i.e. apoCaM. The activation of these channels is medi-ated by conformational changes in the CaM ⁄ SK2 com-plex induced by binding of at least one Ca2+ion ... CaM. Assaying CaMbinding to GST-fusion proteins of the C-terminaldomain of hEAG1 channels, a binding site was locali-zed within amino-acids 673–770. This stretch of resi-dues, harboring a putative ... Ca2+concentration leads to exposure of regions in the CaM ⁄ SK2 complex that induce aninteraction between neighboring SK2-CaM bindingdomains mediated by Ca2+⁄ CaM. This interactionthen...

Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc

... combination of tighter binding of cadmium than zinc in metallothioneinsand the preference of zinc in the more ﬂexible b-domain. In addition to the already described differences in ﬂexibility of the ... metallothioneins. This points to an hitherto unknownmechanism or compound(s) being involved in this inter-action in living cells and information about this additionalfactor is needed in order ... oxi-dation state of thiols is involved in the metal binding in Cd-Sor Zn-S clusters found in metallothioneins, and NO alsobinds to reduced glutathione via S-nitrosation. Using avariety of techniques,...

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