Báo cáo khoa học: Identiﬁcation of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

... ª 2005 FEBS Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis Nate´rcia Conceiçaõ1*, ... mater-nally inherited, and we provide evidence for the pres-ence of alternative promoter usage in this gene during early X. laevis development. Results Identification of a functional proximal promoter for ... (http://www.ncbi.nlm.nih.gov/genome/guide/rat/) MGP genes. Numbersindicate the position of the last nucleotide shown according to the ATG initiation codon of each gene. Alternative promoter usage for Xenopus MGP gene N. Conceiçaõ...

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... attachedto the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transientlyor stably, with lamins, chromatin proteins and a vari-ety of regulatory proteins, including ... occurs between the DNA ⁄ protein com-plexes of the heterochromatin protein BAF and emerin s amino-terminal LEM domain (aa residues 2–44)[38], which similarly exists in the INM proteins MAN1and ... important in the context of a muscledystrophy is a muscle-speciﬁc interaction with the actin-binding spectrin-repeat proteins nesprin 1a andnesprin 2 [28,29].Since the different binding regions...

Báo cáo khóa học: Identiﬁcation of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt

... mutations in 14 distinct resi-dues, all facing the inside of the cell. Five of the residues liewithin the previously characterized N-terminal regulatorydomain and two mutations are located within the ... avoiding any sidechain conﬂicts, and bringing the N-loop in as centrally aspossible to the pore cavity, rotating the ends of the N-looptoward transmembrane domain 1 (the closest TMD in sequence ... mutations of the two prolines P232 and P236, the double mutant (S246P plus stop at Q591), K250E in the linker to the ﬁrst TMD and mutations in G348 in the B-loop. Interestingly, different exchanges of...

Báo cáo khoa học: Identiﬁcation of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues ppt

... C-terminal region of RGS3 comprised the functionaldomain for negative regulation of Gas protein. Sincethen, alternatively spliced RGS protein isoforms weredetected for many RGS proteins. RGS8 alternativelyspliced ... an RGS8s protein with nineamino acid deletion in the N-termini of RGS8 protein [19,20]. The N-terminal deletion of RGS8 resulted in aremarkable decrease in the inhibitory effects of RGS8on ... RGS5s proteins. T, total protein; M, mem-brane protein; C, cytosolic protein. Arrows indicate a 26 kDa protein of RGS5 and a 13 kDa protein of RGS5s. Alternative mRNA splicing variant of RGS5 Y....

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... that RGG3 of EWS binds mainly to the G-quadruplex.To test whether formation of the G-quadruplex isnecessary for RGG3 binding, we assayed the binding of RGG3 with Htelo in the presence of K+or ... RGG domain of nucleolin,a DNA-binding protein contributing to the transcrip-tion of ribosomal RNA, bind to the G-quadruplexforming ribosomal DNA [26]. Moreover, nucleolinbinds to the c-myc ... nucleolin [21–27]. The RGG domain of FMRP, which is an RNA-binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition, the RBD and the...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... a-galactosyltransferase for addition of the terminal galactose of the LOS [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the LOS [26]. However, for lipid Abiosynthesis of the ... containing the various agents were plated on the lawn in triplicate at37 °C for 18 h. Sensitivity was assessed by measuring the diameter of the zone of growth inhibition in two axes, and the ... [41],and the ORFs of these two genes were determined with the Glimmer method [42]. Topology predictions of the deducedproteins were performed using tmpred, toppred and pre-dictprotein [43–45]....

Tài liệu Báo cáo khoa học: Identiﬁcation of differentially expressed genes of the Paciﬁc oyster Crassostrea gigas exposed to prolonged thermal stress docx

... tothermal stress are known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein ... but only in gills. The upregula-tion of CTSL, combined with the downregulation of its reversible binding inhibitor cystatin B, implies thatactive protein degradation was taking place in the gillsupon ... original French government worksdominated by strong downregulation of genes involved in protein synthesis, such as the translation initiationfactor eIF-2B delta subunit, suggesting a slowing of protein...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... differentdomains of the protein, to determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains ... [4–7]. We therefore exam-ined proteins contained in aggregates within the otolith matrix and identiﬁed a protein contained in the HMW protein glycosaminoglycan aggregate thatalso contains the otolith ... expressed in the early neurogenesis stage of the embryo and isinvolved in inner ear development. In omm-64 gene, the sequence encoding OMM-64 isdivided into 23 exons, including two large exons in the middle...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... and the genes encoding theseenzymes. The biosynthesis of triterpene saponins involves the initial cyclization of 2,3-oxidosqualene, a common pre-cursor of all the sterol and triterpene biosyntheses, ... [4–6]. In order to improve the biological production method, adetailed understanding of the biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions ... triterpene-hydroxylat-ing P450 from soybean (Glycine max). G. max is one of the most important crops in the world, and the accumulated EST information revealed the existence of more than 200 types of P450 genes...

Tài liệu Báo cáo khoa học: Identiﬁcation of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc

... imply-ing that the STAT2 DNA binding domain is essential for the IFN-triggered expression of these genes: TLR3,RBMS3. Closer examination of the IFN-inducible expression of the TLR3 gene using ... Thus,IFN-inducible expression of these genes is impaired in the absence of the STAT2 DNA binding domain, sug-gesting that their IFN-inducible transcription requiresISGF3-independent STAT2-containing ... for the expression of these genes. We moreclosely examined the expression of the GBP1 gene asan example of this class of ISG. GBP1 expression wasonly weakly up-regulated in U6A cells but induced...

Xem thêm