Báo cáo khoa học: Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins pdf
Báo cáo khoa học: Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins pdf
... Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins Lorna J. Warnock, Sally A. Raines, Trevor R. Mee and Jo MilnerYCR p53 ... recombinant p53 proteins were expressed in Sf9 insect cells by using the baculoviral system and were purified as described previously [15]. Both human and murine p53 proteins were studied and included ... 111213PAb240K373AcK373/K382AcFig. 2. Phosphorylation status at S15 and acetylation status atK373 and K382 of murine wild-type p53 and p53 proteins mutatedat specific phosphorylation sites expressed in insect cells by usingPAb240...
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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx
... shown that interactions betweenKCs and endotoxin comprise the initiating event lead-ing to hepatotoxicity in liver injury, including endotox-emia and ischemia ⁄ reperfusion injury [14]. In ourstudies, ... mediator of lethal sepsis, triggering theproduction of proinflammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... con-centrations of tumor necrosis factor-a and interleukin-6, and increasedhepatic mRNA levels of tumor necrosis factor-a and interleukin-6 weredecreased by depletion of KCs. Our findings suggest...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx
... have indi-cated that the CagAPY-induced cell elongation pheno-type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well-known actin-binding proteins ... the amount of intracellu-lar CagAPY. Cortactin, ezrin and vinculin are all Srcsubstrates, and Src inactivation causes dephosphoryla-tion of these proteins and is crucial for triggering cellelongation ... phosphatasesinvolved in this scenario, however, remain unknown and do not require Shp2. Instead, CagAPYcan inhibitSrc activity both by direct interaction of both proteins or by binding of CagAPYto...
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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt
... betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation of CERK expression by activa-tion of PPARb results in a decrease ... survival resultingfrom activation of PPARb.Activation of PPARb by LD induces CERK mRNAexpression and increases CerK activity in SP1cellsTo investigate the role of CerK and its regulation of Cer ... CerK results in decreased levels of intracellular Cer, and the inhibition of stress-induced cell death.The interaction between CerK and PPARbmay play an important role in regulating epi-dermal...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
... preparedfrom CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractionsisolated 5 min postinjection of CT-B, and this increasewas maintained for up to 60 min (Fig. ... cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in vitro, the cAMP response to choleratoxin. This cathepsin D-dependent action of cholera toxin under the con-trol of endosomal ... compartmentalization and cytotoxic action of cholera toxin in rat liver parenchyma. Following administration of a saturating dose of cholera toxin to rats, rapid endocytosis of both cholera toxin subunits...
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... vector by using Lipofectamine (Invitrogen).Cells were selected in neomycin, and clones were isolated and screened for Bcl-XLexpression.Creation of TAM67-overexpressing cell lineThe dominant-negative ... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNAbinding, Bcl-XLexpression, ... AP1 DNA binding activity increasedwithin 1 h following EGF treatment and peaked at 3 hfollowing the treatment (Fig. 5A). We also examinedthe effect of PD98059 on this binding activity and, asexpected,...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins. However, two other positively charged amino ... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... albumin(BSA) for calibration.MALDI-TOF-MS analysis of fosfomycin bindingWild-type MurA, the K22V, K22R and K22E singlemutant proteins and the K22V/R120K and K22V/R120Vdouble mutant proteins...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
... onconformation of inhibitor main chain. Thus, differentinhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the bindingtunnel. Some s pecial inhibitors ... that t he strain imposed on inhibitor duringdocking into the binding site of pr otease does not allowcorrect orientation of the hydroxyl group to catalyticaspartates either in S or in the R configuration. ... of inhibitors OE, RE and SE towards their HIV-1 proteases. Stated numbers are distancesbetween inhibitor and protease atoms in A˚.Reside of Atoms of Inhibitor complexInhibitor Protease Inhibitor...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
... five amino acids of theextracellular domain and devoid of any tag was notfunctional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag wasfound ... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantified by measuring thebinding of [125I]Streptavidin. In the absence of ... room temperature for 15 min, thencooled 10 min at 4 °C in cold incubation medium. Theywere then incubated with 0.25 mL of incubation mediumcontaining 2 lgÆmL)1 of biotinylated R5T-34 monoclonalIg...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the ... domain structure of the V. spino-sum tyrosinase in more detail, we determined proteinstability by recording protein unfolding via fluores-cence spectroscopy when increasing amounts of guani-dine ... present not only in tyrosinases, but also in the oxygen transportinghaemocyanin proteins, is the PYWDW (amino acids118–122) and has been hypothesized to be involved in oxygen binding [26].Previous...
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Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)QUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ