... M. Amblar and C. M. Arraiano
374 FEBS Journal 272 (2005) 363–374 ª 2004 FEBS
A single mutation in Escherichia coli ribonuclease II
inactivates the enzyme without affecting RNA binding
Mo
´
nica ... (rnb296)
encodes an inactive RNase II enzyme [25]. In this
report we demonstrate that the single amino acid sub-
stitution Asp209fiAsn in RNase II is able...
... of the
psbA
mRNA is not affected
in the
mf2
strain
The dramatic decrease in D1 synthesis in mf1 and mf2 strains
did not correlate with any significant changes in the
accumulation of psbA mRNA, as ... C-3¢, and Acod (reverse): 5¢-CGC GGA TCC
ATG GAA TCG ATG TAT AAA CGG TTT TCA GTT
GAA GT-3¢,andtheEcoRI restriction fragment of the
chloroplast genome R14 [16] as a template. T...
... A point mutation in the ATP synthase of
Rhodobacter capsulatus
results in differential contributions of DpH and Du in driving
the ATP synthesis reaction
Paola Turina and B. Andrea Melandri
Department ... for assessing
theroleofGlu210intheprotonpathwaywithinF
0
.
Alternatively, it can be t hat the mutation has an even
longer-range effect, affecting t he conformation at the...
... from a thermally unstable
one: frog, toad and newt DNases I all have a Ser205
insertion in a domain that contains an essential Ca
2+
-
binding site in the mammalian enzymes and are thermally
Fig. ... Amphibian
DNasesIhaveaSer205insertioninaCa
2+
-binding site of
mammalian and avian enzymes that reduces their thermal
stabilities [Takeshita, H., Yasuda, T., Iida, R., Nakajima, T...
... from the host protein remained
unchanged. The impaired metal -binding ability caused
by Asp to Ala mutations at positions 1 and 3 echoed a
previous observation that these mutations in the intact
STIM1 ... Ca
2+
signal change and the accompa-
nying conformational change in the canonical EF-hand
are probably relayed to the SAM domain via the
paired ‘hidden’ EF-hand, resulti...
... mole-
cular sieve chromatography. Each protein variant
eluted as a single species; however, all protein variants
exhibited larger elution volumes indicating a lower
apparent mass (Table 2). These data ... Interestingly, all protein variants show a drastically reduced quinone
reductase activity in steady-state kinetics. Detailed analysis of the two half
reactions revealed that the...
... feature specifica-
tions; the latter are themselves items consisting
of a feature name and a feature value in an ar-
rangement. The formal devices already introduced
allow us to state cooccurrence ... restrictions gov-
erning the combination of features and values in
feature specifications; the definition of the
value range of a feature can thus be regarded as
anot...
... demon-
strate a strong conservation in the binding of C-terminal binding protein-
interacting domains despite great variability in their amino acid sequences.
Finally, this L22 5A point mutation could also ... with the structural data
and the universal conservation of this residue in all C-terminal binding pro-
tein-interacting motifs, mutation of the central leucin...
... years,
to anoxia and diapause, conditions that are invaria-
bly accompanied by large increases in intracellular
Ca
2+
[3].
Despite intense research on the mammalian PTP
since its characterization by ... for 10 passages. The homog-
enate was centrifuged for 10 min at 300 g and 4 °C, the
upper fatty layer of the supernatant was aspirated, and the
remaining supernatant was centrifuge...