Báo cáo khoa học: Stability of the major allergen Brazil nut 2S albumin (Ber e 1) to physiologically relevant in vitro gastrointestinal digestion doc

... physiologically relevant in vitro model sys-tem either before or after heating (100 °C for 20 min). Whilst the albumin was cleaved into peptides, these were held together in a much larger struc-ture even ... DESCRCEGLRMM(20– 31) of the large subunit (Table 1). Pepsinolysis removed either the N-terminal Asp or the C-terminal Met, giving rise to peptides ESCRCEGLRM(21–30) and ESCRCEGLRMM(21– 31), respectively. These ... (gastric) digestion. They also containedpreviously identiﬁed putative T-cell epitopes. These ﬁndings indicate that the characteristic conserved skeleton of cysteine residues of 2S albumin family...

Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"

... disaster in southeastAsia severely injured tourists were repatriated via airlift to Germany. One cohort was triaged to the Cologne-MerheimMedical Center (Germany) for further medical care. We reporton ... vacuum-assisted closure in the interim between initial wound surgery and secondary closure. Allpatients received empiric anti-infective therapy using quinolonesand clindamycin, later adapted to incoming ... the University of Heidelberg (Germany) assembled a comprehen-sive list of 400 qualified psychotherapists offering immediatesupport nationwide when needed. These structures were notpresent prior to...

Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... post-tran-scriptional effects, and most likely changes in proteinsecretion, the mRNA levels of each lysozyme variantrelative to the endogenous genetic reference b-actin,were determined by reverse transcriptase ... correlation has been identiﬁedbetween the level of secreted protein and the thermo- stability of the native state of the protein (Fig. 2A).This correlation shows that even small changes in the protein ... not detected in the urine or plasma of these patients [24]. In the case of the F57I variant, amyloid deposits were present in patients possessing the F57I genetic mutation and in one case a second...

Báo cáo khoa học: Effects of the G376E and G157D mutations on the stability of yeast enolase – a model for human muscle enolase deﬁciency pdf

... glycine is nonconservative, we introduced alanineat these two positions, with the aim of determiningwhether any of the observed effects were a result of changes in the size and charge of the ... structure, observed in the CD spectrum,affects this part of the protein, or whether there istransient unfolding of the end of this helix. There areexamples of helices in proteins that undergo ... differences between wild-type enolase and the variants, indicating that the variants were folded cor-rectly. However, there were signiﬁcant differences in the aromatic region. The spectrum of the...

Báo cáo khoa học: Analysis of the stability of the spermadhesin PSP-I ⁄ PSP-II heterodimer Effects of Zn 2+ and acidic pH pdf

... obtained by MALDI MS (28 664 Da) by the number of amino-acid residues of the mature proteinsequence (225 residues).Thermal denaturation experiments were carried out byincreasing the temperature ... behaviour.Discussion The near-UV CD spectrum of PSP-I ⁄ PSP-II reﬂects the speciﬁc environment of chiral aromatic side chains in the tertiary structure of the folded protein, and the band intensities decrease in ... the heparin and mannose 6-phosphate bind-ing sites of PSP-II, which have been proposed to belocated at the heterodimer interface [20], may not beexposed in the male genital tract.On the other...

Báo cáo khoa học: Effect of the -Gly-3(S)-hydroxyprolyl-4(R)-hydroxyprolyltripeptide unit on the stability of collagen model peptides ppt

... collagen-like peptides increases the stability of the triple-helical structure by a small margin.Insertion of 3(S)Hyp in the context of the ninetripeptide units increases the Tm of the peptides ... indicates the importance of 4(R)Hyp in the nucleation process. Future studies willbe performed to study the mechanism of refolding of these model peptides in detail. In order to evaluate the thermodynamic ... model peptides are in uenced mainly by the residue in the Yaaposition of the -Gly-Xaa-Yaa- repeated sequence. The experiments indicatethat replacement of a Pro residue by a 3(S)Hyp residue in...

Báo cáo khoa học: Role of the surface charges D72 and K8 in the function and structural stability of the cytochrome c6 from Nostoc sp. PCC 7119 potx

... name of the corresponding mutants.Error bars represent errors from the fit to determine kbim. The solidline represents a linear fit to the data points, dashed lines mark the 95% confidence intervals. ... between logkU0and the Gibbs energy of unfolding at 0 [urea](DGU0). In the mutant series, the overall height of the energy barrier for the rate-determining step of the fold-ing ⁄ unfolding ... mainly determined by the overall difference in Gibbs energy between the nativestate (N) and the ensemble of unfolded states (U), in agreement with a single transition state with a disor-dered...

Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

... partially to these vari-ations.ResultsSteady-state levels of cyclin mRNA in the cellcycle In a ﬁrst series of experiments we determined whethermRNA steady-state levels of cyclins and several cellcycle-related ... cells,essential regulatory steps are likely to be conserved in evolution and thus amenable to genetic analysis in the mouse. We therefore examined mRNA expression and stability in synchronized ... mRNAs,which have quite long half-lives, raises the question of how their levels decrease at the end of M-phase. Wehave to assume a prolonged period of transcriptioninhibition to explain the decay of an...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... the single-molecule level. The analyses of individ-ual SERCA molecules in the presence of both ATP and free Ca2+revealedup–down structural changes corresponding to the Albers–Post scheme. Thisﬂuctuation ... of the projection of the embed-ded molecule above the ﬂat membrane surface, the average height was 5.4 ± 0.8 nm, which is in goodagreement with the height of the cytoplasmic domainestimated ... conformation (i .e. peakwidth) in the presence of both ATP and Ca2+wasmeasured in the single line FSAFM images, andFig. 1. Single-molecule imaging of SERCA dynamics in the presence of nucleotide and...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... Dozens of bacte-rial factors have been identiﬁed to in uence the patho-genesis of H. pylori. There are two classical secretedvirulence factors present in H. pylori: the vacuolatingcytotoxin ... triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu. (B) Model-1 for the assembled T4SS machinery ... cell signalling. Another unsolved question is the proposed structure of CagY with respect to whichdomain is exposed to the extracellular space. The repeatdomain in the middle of CagY has been...

Xem thêm