... Antisense glutaminase inhibition decreases glutathione antioxidant
capacity and increases apoptosis in Ehrlich ascitic tumour cells
Jorge Lora, Francisco J. Alonso, Juan A. Segura, Carolina ... normal cells. In the present
work, Ehrlich ascites tumour cells, and their derivative,
0.28AS-2 cells, expressing antisense glutaminase mRNA,
were assayed f...
... counting.
Preparation of cell extracts and analysis
by immunoblotting
Cytoplasmic extracts were prepared for immunoblotting by
washing the cells in NaCl/P
i
and lyzing them in a buffer
containing ... mutant
in MEL cells (or of the equivalent Val138 mutant in human
cells) can be achieved by reducing the incubation tempera-
ture from 38 to 32 °C and results in inhibition...
... is noninfective and
proliferates extracellularly in the insect gut where it
differentiates into metacyclic trypomastigotes, which
can then infect the mammalian host cells and replicate
intracellularly ... con-
taining DNA spots (5 lg each) corresponding to inter-
nal fragments of ADC or cruzipain genes. The latter
(used as a control) is a housekeeping gene that encodes
the main cystei...
... constructs,
spectinomycin (100 lgÆmL
)1
)wasused.
Proteins, GAGs, radiolabelling and binding assay
Recombinant protein H, M1 protein and the A-S and S-C3
fragments of M1 protein were prepared ... region, IgGFc-binding to the S-domain, and albumin-binding to the C-repeats [31].
Recombinantly expressed fragments rA–S and rS–C3 are indicated. (D) The M1 protein and the A–S and S–C3...
... proteinase with a limited distribu-
tion in certain cell types, including gastric epithelial
cells [4], but is mainly present in cells of the immune
Keywords
antigen-presenting cells; cathepsin ... brain [23].
CatE and CatD both play an important role in the
MHC class II pathway. CatD is reported to be
involved in processing MHC II-associated invariant
chain [24] in antigen p...
... (Ala253 and Val254 in LDH are replaced by
Val253 and Ile254 in LDHB). The histidine and argi-
nine residues directly involved in catalysis are identical
in the two enzymes, and NADH binding is ... present in the parental strain
and in the mutant FI9078 were due to homofunctional
enzymes displaying clear differences in kinetic and reg-
ulatory parameters. Therefore, w...
... dissected
and preincubated in Ringer’s medium for 15 min at 22 °C.
Radioactive labeling of newly synthesized proteins was per-
formed by incubating the NILs in Ringer’s medium con-
taining 5 mCiÆmL
)1
[
35
S]-Met ... been studied only in cultured (transfected) tumour cell lines and not in
primary cells. Here, we investigated the intracellular fate of PrP
C
in vivo by
using th...
...
chitecture is distinguished from these in its
treatment of discourse-level processing.
Most architectures, including ours, contain mod-
ules for speech recognition and natural language
interpretation ... Tracking
The Context Tracker maintains a record of the
discourse context which it and other components
can consult in order to (a) resolve dependent
forms that occur in inp...
... seedlings [27].
Macropinocytosis dependent transduction of fluores-
cent proteins by arginine-rich intracellular delivery
(AID) and Tat-protein transduction domain has been
reported in onion and ... combined use of cell per-
meabilizing agent and cell-penetrating peptides results
in the delivery of such large sized cargoes in plant cells
involving endocytic ⁄ macropinocytic pathw...
... nitrate in air. (F) Ornithine, citrulline and arginine contents in leaves of Arabidopsis plants cultured
with 2 m
M ammonium in air. (G) Ornithine, citrulline and arginine contents under high-CO
2
conditions. ... overall arginine levels.
The levels of ornithine, citrulline and arginine
remained low, at between 0.01 and 0.04 lmolÆg
)1
fresh
weight (Fig. 3E). However, arginine acc...