Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... cysteine and/or histidineresidues, forming a zinc finger domain that is mostly involved in DNA binding but also in protein–proteininteractions [17]. Originally identified as being present in different ... the coupling of zinc- binding structures (either in MT or in zinc finger proteins) by selenium compounds with the cellular r edox system GSH/GSSG, reducible selenium may be regenerated within catalytic ... folding of the minimal DNA- binding domain [50]; substitution of any of its zinc- complexing cysteines (Cys105, Cys108, Cys126 andCys129) leads to diminished DNA binding and a severereduction in...
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Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc
... indicatethat the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, NMRmeasurements showed that in solution the spectra of GA ... thermodynamic parameters obtained are in therange of those of other interactions with c-CD [15].The interaction of GA with c-CD is accompanied byfavorable DS values together with favorable DH values(Tables ... and b-CD are toosmall. In order to determine the binding site of GA,binding of glucuronic acid to c-CD was also analyzed,although that of gymnemagenin could not be examineddue to its low solubility....
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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... that contains specificbinding sites for the lyn kinase [31]. These findings provide arationale for investigating whether caspase-mediated path-wayscaninteractwithPDE5inintactcells. In this article, ... plasmid construct followingcomplex formation with LipofectAMINETM2000, accord-ing to the manufacturer’s instructions. The cDNA contain-ing media was then removed following incubation for 24 hat ... catalytic competence in PDE4 and to play a keyrole in the binding of competitive PDE4 inhibitors [36]. Theside chain of this residue may conceivably p-stack with thepurine base of the bound substrate...
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Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc
... HsRad51 toexamine whether these loops are actually involved in DNA binding. Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA- binding protein (SSB) and ... 3149alanine. If the aromatic side chain is involved in the interaction with DNA, then its replacement with alanine, a short side chain amino acid residue,should affect the DNA binding of the protein. ... Rad57 in DNA repair by increasing the stability of Rad51 -DNA complexes. EMBO J 21, 3160–3170.45 Morimatsu K & Horii T (1995) DNA- binding surface of RecA protein photochemical cross-linking of...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... polymerization of intact actin. (A) Effect of HSP25 and its 3D mutant on the kinetics of actin polymerization.Samples containing 1–4 lM of actin (10% of pyrene-labeled protein)were incubated for 5 min in ... theeffect of HSP25 on the polymerization of intact actin andaggregation of heated actin. Interaction of HSP25 with intact actin In the first series of experiments we analyzed the effect of HSP25 ... salt-induced increase of the light scattering (B) of actin. Actin (15 lM)containing 10% or pyrene-labeled actin in buffer G were incubated at 4(1), 43 (2), or 60 °C (3) for 1 h. After cooling...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... feedback-inhibited form of GSbinds tightly to TnrA, preventing its binding to DNA, with the most effective feedback inhibitors of GS beingglutamine and AMP [9]. Mutations in TnrA that result in constitutive ... coimmunoprecipitation (View interaction) ltnrA physically interacts with glnK by anti bait coimmunoprecipitation (View interaction) ltnrA binds to tnrA by cross-linking study (View interaction) ltnrA binds to ... truncations.Fig. S3. Crosslinking analysis of truncated TnrA proteins. Doc. S1. Purification of His6-tagged TnrA proteins, purification of GlnK-ST and GS-ST, and glutaralde-hyde crosslinking assays.This...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... containing amino acids 98–110 of the flavivirus E protein) may serve as an internalfusion peptide, as it is directly involved in interactions with target membranes during the initial stages of membrane ... the interactions of anN-terminal and an internal peptide sequence of the E2structural protein of HGV ⁄ GBV-C with model mem-branes, in order to understand the possible mode of penetration of ... the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of membrane fusion intermediates are poorly under-stood [2]. In this...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... 17.6Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the ... [15]. In this study, we measured the interaction of stefin B with various combinations of phospholipid monolayersand bilayers. Interaction of stefin B in the prefibrillaraggregated state with model ... binding of the aggregated forms,albeit to a much smaller extent than that observed forsome proteins that bind specifically to membranes,such as the small membrane-binding domains involved in...
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Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx
... ND49 with CP is because of deletion of the interactingdomain.DiscussionMPs are a diverse group of non-structural proteins of plant viruses that are involved in the spread of infectionfrom ... major interacting domain within theN-terminal 49 amino acids of the SeMV MP. This is in contrast to observations on CPMV MP, in whichthe C-terminal domain was shown to be involved in NV recognition ... affinity chromatography (Fig. 7A).Mapping of the SeMV MP domain necessary for interaction with NVTo determine which domains are involved in the inter-action of MP with NV, ELISA was performed asdescribed...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... amount of recovered protein owing tothe presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the proteinwas incubated with 1 ng of the indicated ... form with it the 100-kDa complex[21]. Figure 3B shows the binding of labelled oligomersto the nuclear-enriched fraction of proteins. The effi-ciency of labelling of the single oligomers being ... ratios of the different binding signalswithin each lane instead of their absolute values haveto be considered. It can be seen that GT is presentmainly in the CRC and in a minor complex of about100...
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