Báo cáo khoa học: Structural and functional analysis of ataxin-2 and ataxin-3 potx

Báo cáo khoa học: Modular metabolic control analysis of large responses The general case for two modules and one linking intermediate docx

... concentration of itsproduct and the sensitivity of the rate of the demandmodule to changes in the concentration of its sub-strate, respectively.In the case of mean elasticity coefﬁcients, pi and Sboth ... concentration of the intermediate,S, and measure the rates of the supply and demandmodules, v1 and v2. Applying ﬁtting procedures to thetable of experimental values v1, v2 and S, continuousfunctions, ... 2B).Next, we representCSv1 and CJv1as a function of r1, and CSv2 and CJv2as a function of r2in two parametricplots:CJv1 and CJv2in Fig. 3A and CSv1 and CSv2inFig. 3B. These...

Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1[16,18]. The temporal and cell-specific expressionpattern of Anp32 in cerebellar ... regions of the molecule.As part of a long-term function-oriented structural effort aimed at understanding the molecular bases of polyQ disease proteins, we report here a study of the structural ... Theidentification of phosphorylation sites of pp32 and bio-chemical purification of a cellular pp32-kinase. Biochem-istry 43, 10157–10165.27 Bairoch A (1992) PROSITE: a dictionary of sites and patterns...

Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf

... foreach edge of the graph.6: Reﬁne the scoresS1 and S3 of the k2sentiment and topic word candidates using Eqs. (5) and (6) iteratively.7: Select k1new sentiment words and k1new ... set of nodesrepresents topic words, including new topic candi-dates and words in the lexicon C, and the other set of nodes represents sentiment words, including newsentiment candidates and ... k2sentimentwords and topic words with highest scores as candidates.5: Construct a bipartite graph between sentiment and topicwords on DlT and the k2sentiment- and topic- word can-didates, and calculate...

Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx

... needed for directmeasurement of holo-TC in serum. The antigenicdeterminants and the functional domains of TC havenot been identiﬁed.Cloning [5–7] and recent expression of several kind-red Cbl-binding ... absence of S-S bonds and the low number of conserved residuesin this part of the sequence. Only later did the largeN-terminal unit become involved in the binding of Cbl [13,14]. Assembly of two ... 3C4 and 2-2 ⁄ 3C4, where binding of the second mAb of thepair was inhibited by < 20%.Interaction of mAbs with the C-terminal domain of human TC was determined using peptides TCp11 and TCp12,...

Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reﬂection on some controversial features Elena Ghibaudi and Enzo Laurenti docx

... features of the LPO and MPO catalytic cycle, such as theexistence of Compound I and Compound II isomers and the identiﬁcation of their spectroscopic properties. Afteraddressing each of these ... the catalytic cycle of twomammalianperoxidases,LPOandMPO,inordertoreconcile the apparent inconsistencies and to provide somenew insights. MPO and LPO share functional and structural homology, ... each of these questions, we will proposea new hypothesis that describes an integrated vision of thecatalytic mechanism of MPO and LPO.A brief survey of the absorption features of MPO, LPO and...

Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx

... the majority of itsactivity over the same time period.CD analysis of PrP mutantsIn order to determine if deletion of critical regions of PrPcaused loss of activity because of structural alterations, ... activity of PrP.Production of deletion mutants of PrPOn the basis of the results with antibodies, a series of PrPmutants were made to assess whether deletions of certaindomains of the protein ... Theresults of these experiments are shown in Fig. 1. Several of the antibodies and antisera caused a concentration-dependent inhibition of the SOD-like activity of PrP. Theability of the antibodies and...

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... transcription–PCR analysis of unknown serial analysis of geneexpression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... PCR analysis of unknown tags on serial analysis of gene expressionWang-Jie Xu1, Qiao-Li Li1, Chen-Jiang Yao1, Zhao-Xia Wang1, Yang-Xing Zhao1 and Zhong-Dong Qiao1,21 College of Life ... that alarge number of SAGE tags do not match the existingexpressed sequences upon analysis of the SAGE dataKeywordsmodiﬁed lock-docking oligo(dT); mRNA;RACE; serial analysis of gene expression(SAGE);...

Báo cáo khoa học: "Bilingual Hebrew-English Generation of Possessives and Partitives: Raising the Input Abstraction Level" pptx

... different syntactic constraints of the two languages. We present a contrastive analysis of the syntactic realizations of possessives and partitives in Hebrew and English and conclude by presenting ... 1994)): measure a mile of cable, typical partitives a loaf of bread, a slice of cake, and general partitives: a piece/bit /of an item of X. In the syntactic structure of a partitive structure, ... amount of the thing. The main modifications from the existing SURGE input structure are the introduction of the re/-set feature and the update of the usage of the possessor feature. For both of...

Báo cáo khoa học: Modular metabolic control analysis of large responses in branched systems – application to aspartate metabolism potx

... within the framework of metaboliccontrol analysis (MCA) [1–5].Metabolic networks of organisms show thousands of variable concentrations and fluxes, so full application of traditional MCA to ... definitions of the coef-ficients in Doc. S1 and [21]). The number of responsecoefficients in cellular metabolism (number of parame-ters · number of variables) is very large, and measuringall of them ... the values of the factors r1, r2 and r3 and of the control coefficientsas a function of S. Finally, the parametric plot of thecontrol coefficients for large changes as a function of ri(i...

Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf

... approachwould be the early recognition of ‘off target’ and sideeffects of drug candidates [7], as well as the identiﬁca-tion of putative biomarkers.The phosphorylation status of signaling pathwaycomponents ... dynamic aspects of pathway events and (d) the proﬁling of compounds to reveal signaling and cross-pathway effects of drug candidates. In addi-tion, analysis of healthy versus diseased tissue ... modulation of phosphorylation onTyr, Ser and Thr residues on each other and on a vari-ety of downstream effectors. Aberrant cellular signal-ing is a hallmark of many diseases, and consequentlythere...

Xem thêm