Báo cáo khóa học: Type 2 isopentenyl diphosphate isomerase from a thermoacidophilic archaeon Sulfolobus shibatae potx

... Type 2 isopentenyl diphosphate isomerase from a thermoacidophilic archaeon Sulfolobus shibatae Satoshi Yamashita, Hisashi Hemmi, Yosuke Ikeda, Toru Nakayama and Tokuzo NishinoDepartment ... primers 5¢-TAAATCATGATAACGGGCATGACTGG-3¢ and 5¢-TTAAGGGATCCATATTCTTCTCTTTCTAAC-3¢. The genome of S. acidocalda-rius, as the template, and KOD DNA polymerase (TOY-OBO) were used for the reaction. ... BiomolecularEngineering, Graduate School of Engineering, Tohoku University,Aoba-yama 07. Sendai, Miyagi 980–8579, Japan.Fax: + 81 22 21 7 729 3, Tel.: + 81 22 21 7 727 2,E-mail: hhemmi@seika.che.tohoku.ac.jpAbbreviations:...

Tài liệu Báo cáo khoa học: "Learning to Compose Effective Strategies from a Library of Dialogue Components" doc

... reward expected be-tween taking that action and the end of the dialogue.For each state-action pair (s, a) , we calculated thisexpected cumulative reward Q(s, a) of taking action a from state ... that particular measure), and a policy based on a combination (sum) of the rewards for all evalu-ation measures. We found that the learned policybased on the combination of all measures, and ... reinforcement learning for auto-matic dialogue policy optimization in a question-based motivational dialogue system. Our system canautomatically compose a dialogue strategy from a li-brary of dialogue...

Tài liệu Báo cáo khoa học: "Modelling Early Language Acquisition Skills: Towards a General Statistical Learning Mechanism" potx

... 1999; Saffran et al., 20 00; Kirkham et al., 20 02; Anderson et al., 20 03; Seidenberg et al., 20 02; Kuhl, 20 04; Hannon and Trehub, 20 05). Experiments by Saffran et al. (1996, 1999) show that 8-month ... downfall of the CELL approach is that it assumes speech is ob-served as an array of phone probabilities. A more radical approach is Non-negative ma-trix factorization (NMF) (Stouten et al., 20 08). ... repeated 10 times and recorded with 4 different speakers (2 male and 2 female) to produce 4000 utterances. In addition to the acoustic data, each utterance is associated with an abstract semantic...

Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx

... bodies E. Garcı´ a- Fruito´s et al. 24 22 FEBS Journal 27 8 (20 11) 24 19 24 27 ª 20 11 The Authors Journal compilation ª 20 11 FEBSaggregated state and the way in which this supramolec-ular ensemble ... core [24 ].DnaK, which participates in the in vivo refolding ofbacterial thermal aggregates [25 ,26 ], appears to behighly active on bacterial IBs [20 ,27 ,28 ]. In fact, wehave recently shown that ... deBioingenierı´ a, Biomateriales y Nanomedicina (CIBER-BBN, Spain), an initiative funded by the VI NationalR&D&i Plan 20 08 20 11, Iniciativa Ingenio 20 10, Con-solider Program, CIBER Actions and ﬁnanced...

Báo cáo khoa học: Functional implications of pigments bound to a cyanobacterial cytochrome b6f complex potx

... features around 630 and 620 nm, respectively,as well as a sharp negative feature at 555 nm and pos-itive features near 548 and 530 nm. These data indicatenegative LD values for the Qytransitions ... therapid turnover and assembly of photosystem II in Chla-mydomonas reinhardtii. FEBS Lett 400, 359–3 62. 25 He Q & Vermaas W (1998) Chlorophyll a availabilityaffects psbA translation and ... PhotochemPhotobiol 62, 367–3 82. 23 Moskalenko AA & Karapetyan NV (1996) Structuralrole of carotenoids in photosynthetic membranes.Z Naturforsch 51c, 763–771. 24 Trebst A & Depka B (1997) Role of carotene...

Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

... ATG ATG AAG AGC CGGAAT GCC ACC AAA GAT CGA TGC AAG C-3¢ BsmIrev 5¢-G CTT GCA TCG ATC TTT GGT GGC ATT CCG GCT CTT CAT CAT C-3¢L35S fw 5¢-G ATG ATG AAG AGC CGGAAT TCC ACC AAA GAT CGA TGC AAG ... 6-FAM-dArU(dA) 2 -6-TAMRAas substrate [23 ], revealed that all RNase A variants areactive (Table 3). However, while the RNase A variants withmutations in the Ala20 loop region a s well as N ... AGC TCC AAC TAC TG3¢ SacIrev 5¢-CA GTA GTT GGA GCT CAG GGC GGC GGA AGT GCT GGA G-3¢N34D fw 5¢-C CAG ATG ATG AAG AGC CGG GAC CTG ACC AAA GAT CGA TGC-3¢ No restriction siterev 5¢-GCA TCG ATC TTT...

Báo cáo khoa học: "Extraction of Tree Adjoining Grammars from a Treebank for Korean" pdf

... been taken during last decades. Automatic grammar development means that a system extracts a grammar from a Treebank which has an implicit Treebank grammar. The grammar extraction system takes ... natural language processing. Since traditional manual grammar development is a time-consuming and labor-intensive task, many efforts for automatic and semi-automatic grammar development have ... morphological analysis in SJTree allow us to extract syntactic fea-tures automatically and to develop FB-LTAG. Automatically extracted FB-LTAG grammars eventually use reduced tagset because FB-LTAG...

Báo cáo khoa học: "Automatic Acquisition of Script Knowledge from a Text Collection" docx

... Technology 425 9 Nagatsuta-cho, Midori-ku,Yokohama, JAPANfujiki@lr.pi.titech.ac.jpJAPANoku@pi.titech.ac.jpnanba@its.hiroshima-cu.ac.jpAbstractIn this paper, we describe a method forautomatic acquisition ... of Technology 425 9 Nagatsuta-cho, Midori-ku,Yokohama, JAPANHiroshima City University3-4-1 Otsukahigashi,Asaminami-ku, Hiroshima,Manabu OkumuraPrecision and IntelligenceLaboratoryTokyo Institute ... first explainwhat we mean by 'action', 'pair of actions', and'sequence of actions' in this paper. In this work,an action is defined as a tuple of a transitive...

Báo cáo khoa học: "Language-independent bilingual terminology extraction from a multilingual parallel corpus" pdf

... chunks:tableau de commande control panelclimatisation air conditioningcommande controltableau panel 2. combine each NP + PP chunk:commande de climatisa-tion automatiqueautomatic air condition-ing ... translations ofthe source words. Identical strings in source andtarget language are also linked.3 .2 Linking Anchor chunksCandidate anchor chunks are selected based on theinformation available ... freely availableTreeTagger tool (Schmid, 1994) and we used Tad-Pole (Van den Bosch et al., 20 07) to annotate theDutch corpus.In a next step, chunk information was addedby a rule-based language-independent...

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... a plant a -type PLD has been obtained on the basis ofrecombinantly produced PLDa2 from cabbage. SAXSanalysis (Fig. 2) and analytic size exclusion chromato-graphy indicate unequivocally that native ... in the absence and presenceof NaCl and CaCl 2 at pH 5.5 and 23 °C are compared.The results show that the instability of PLDa2isBC A DFig. 4. Spectra of native PLDa2. (A) UVabsorbance spectrum. ... of the data from (A) in the range 0–100 mM Ca 2+ . (C) Modiﬁed Scatchardplot of the data from (B). DA corresponds to the change in relative activity. The PLDa2 activity was measured against PpNp...

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