Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx
Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx
... compilation ª 2006 FEBS 535 Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein Laura Villarreal1, Laura Tı´o2, Merce`Capdevila1 and Sı´lviaAtrian21 ... evolution of the spectra when the indicated number of Cd(II) was added. (B) CD spectrum of the acidification of the final solution of the Cd(II) titration of Zn–ckMT, the arrow shows the evolution ... of the ckMT,ackMT and bckMT metal complexesSpectroscopic (UV-Vis) and spectropolarimetric (CD) analy-sis of the metal ckMT clusters and of the species formedin vitro during the Zn ⁄ Cd and...
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Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx
... methylation [8], and the cleavage of DNA by alkali [31]. The method allows easy detection of DNA–protein interactions via the N7 position of guaninesin the major groove and via the N3 position of adenines ... structure and gene activity. The MMTV-LTR contains potential regulatory elementswhich mediate transcription in the presence of glucocorti-coid ligands and in the presence of androgen, progesterone and ... acetylation of atransactive factor(s), which might lead to specific binding to the distal part of the MMTV-LTR and thereby direct the translational nucleosome positioning. The acetylation status of histones...
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Báo cáo khoa học: Structural adaptation to low temperatures ) analysis of the subunit interface of oligomeric psychrophilic enzymes pdf
... interpreted in the light of the work of Bahadur et al. [36]. These authors stud-ied the subunit interfaces of 122 homodimers, and showed that the distribution of the area between the rim and core ... definition, null. The mean and the standard deviation (Eqns 1–3) of the distribution of the dif-ferences of the structural properties calculated between eachpossible pair of psychrophilic and homologous ... The trend was strengthened by the inclusion of thermophilic enzymes in the comparison.Figure 2 shows the percentage of apolar interface,Table 3. Statistical analysis of the differences in the...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... arresting the cells in the early S phase with adouble thymidine block. We released cells from the block and fixed them at the time points indicated inFig. 5C, and then analyzed the DNA contents of the cells ... cells(Fig. S1). On the other hand, the northern blotting and quantitative real-time PCR analysis also showed that the expression of differentiation marker genes such asfgf5, gata4 and gata6 was not ... the immunoreactivity of PTB both in the ICM and in the trophectoderm (Fig. 2A). In contrast, the expression of Oct3 ⁄ 4 and Cdx-2 was restricted exclusively to the ICM or the trophectoderm (Fig....
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Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx
... evaluation of all the variables in the Hg-MTsystem required the performance and analysis o f 18titrations and the corresponding duplicates. The detailed and comparative analysis of the set of CD, ... n the effect of two variables, the reaction time and the presence of chloride ions, on the stoichiometry, stereochemistry and degree of folding of the Hg(II)-MT species formed by either the binding ... stereo-chemistry and degree of folding of the species formed. Thus,to understand the reaction pathways followed by Zn-MT and apo-MT species in the presence of Hg(II), the e ffect of each of the previous...
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Tài liệu Báo cáo khoa học: "Going Beyond AER: An Extensive Analysis of Word Alignments and Their Impact on MT" pdf
... tables, the distribution of the phrases that are used in decoding, and the cover-age of the test set with the generated phrase tables.Size of Phrase Tables The major impact of using different alignments ... row of Figure 3 shows the per-centage of phrases used to decode the ChineseMTEval’2003 test set. The distribution of the ac-tual phrases used in decoding is completely the re-verse of the ... show the alignment links, and rectangles show the possible phrases.In Figure 1, the first box represents a manualalignment and the other two represent automatedalignments A. In the case of a...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
... Although the number of dual-targeted proteins is small in terms of the totalorganelle proteomes, it is unclear whether this justrepresents the tip of the iceberg. Irrespective of the total number of ... of means, of which alternative splicing of genes and protein modifi-cation are the best characterized to date [1–3].Another mechanism to increase the complexity of proteomes is the editing of ... an outline of the future challenges in this field is provided, and the insights that may be gained from a greater under-standing of the mechanism of dual targeting for the targeting of location-specific...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... implications of the behavior of thisloop. The first is the facilitation of the conformationalrearrangements and the maintenance of the conforma-tional plasticity for effective binding of the protein ... 1.1H,15N-HSQC spectra of the Mdomain of human eRF1. The numbering of the residues corresponds to that of the fulleRF1 protein. (A) The Gly region of the 1H,15N-HSQC spectrum of the M domain of human ... at the PTC of the large ribosomal subunit [20]. The three-domain structure of eRF1, with the shape of the protein resembling the letter ‘Y’, partly mimics the ‘L’-shape of the tRNA molecule, and...
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Báo cáo khoa học: Glycolysis in Entamoeba histolytica Biochemical characterization of recombinant glycolytic enzymes and flux control analysis ppt
... reconstruction of the final stages of amoebal glycolysis Analysis of the kinetic properties of the recombinantglycolytic enzymes indicated that EhPPDK and EhP-GAM had the slowest activity and were the ... conditions.Thus, the kinetic parameters were measured at 37 °C, the temperature at which amoebas grow in vitro and in the host, and at optimal pH and at pH values of 6.0 and 7.0. The Vmaxvalues of the His-tagged ... values, analyzing the effect of activators and inhibitors, and investigating the storage stability and oligo-meric state. Determination of the catalytic efficiencies at the pH optimum and at pH values...
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Báo cáo khoa học: "Augmented Dependency Grammer: A Simple Interface between the Grammer Rule and the Knowledge" pptx
... in analysis and synthesis. It also explains the gap in semantics and logical meaning, and gives a clear computaional image of what we call conceptual analysis. This grammar is used for analysis ... used for analysis of Japanese and synthesis of English, in the Japanese-to-English machine translation system called VEN~S (Vehicle for Natural Language Understanding and Synthesis) currently ... different kinds of information sources other than the lexicon support language comprehension, and two inference functions defined on them extract the interpretation of input sentences. The top level...
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