Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

... 2617 Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA Andrey Kropotov1,2,3, ... 37–45.18 Kropotov AV, Grudinkin PS, Pleskach NM,Gavrilov BA, Tomilin NV & Zhivotovsky B (2004)Downregulation of peroxiredoxin V stimulates forma-tion of etoposide-induced double-strand DNA breaks.FEBS ... basal expression of the PRDX5 gene contributes to the antioxidant defense of the human genome. The 8-oxoG content of nuclear DNA from mouse tissues estimated by quantitative analysis(HPLC and...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is envis-aged. This is ... dispute over the metal content of the RNR of C. ammoniagenes is resolved. In the presentstudy, our strategy was to establish the homologous expression of the C. ammoniagenes nrdF gene and enrich the ... by ESI-QTOF-MS.In our view, C. ammoniagenes restricts the incorpo-ration of iron into R2F in vivo, even in the absence of manganese, and it is the availability of manganese thatis the limiting...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... maintaining the geometry of the active site through the contact among subunits [31].Neighbouring subunits are necessary to prevent waterpenetration into the active site and provide rigidity to the ... (short-term) of poikilotherms to temperature very often leads to chan-ges in two main traits of some metabolic enzymes:quantitative and qualitative. The quantitative properties (concentration and, ... acclimation to low (5 °C) and high (18 °C) temperatures leads to differential expression of alternative forms of the LDH-A gene in white skeletal muscle of weatherﬁsh, Misgurnus fossilis.Two isoforms of...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... contentby ﬂow cytometry revealed that the inhibition of cellgrowth was due to a decrease in the percentage of cellsin the S and G2 ⁄ M phases of the cell cycle (Fig. 3B).In a total of seven separate ... receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modiﬁed proteins. These results are inaccordance with previous in vitro ... class I and II, CD1d, ICAM-1 and the novelCD8 ligand gp180. We also characterized parameters of oxidative stress, cell growth and lipid metabolism in the iron loaded HepG2. The results of the studyrevealed...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... Ca2+independent.Considering all the different pathways reported for the activation of Ras and ERK1 ⁄ 2 downstream of GPCRs, Ras-GRF1 could be one of possibly severalGEFs involved in the activation of Ras and subse-quently ... Research Council of Norway, The Novo Nor-disk Foundation, Anders Jahre’s Foundation for the Promotion of Science, The Blix Family Foundation and grants from the University of Oslo and the NationalCancer ... receptors [8,9]. The main mechanism for the activation of Ras-GRF1 is the binding of Ca2+⁄ CaM to the N-terminalIQ motif [10]. We have previously shown that the treatment of NIH3T3 and COS-7...

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... expansion of T-cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showedproliferation of human T-cells bearing Vb1, V b2, Vb3,Vb5.2, Vb12, Vb15 and Vb17 domains and found differ-ences in the ... of several pathologies and because of the potential use of the toxins as biological weapons. Alteration of their MHC and TCR binding capacity by site directedmutagenesis could be useful in the ... both the mutant and monomeric wild type SSA have aﬃnity for human Vb5.2 and V b1withKd of 9–11 lMwith a fast kass and a moderately fast kdiss. In spite of the reported stimu-lation of V b2.1...

Báo cáo khoa học: EcR expression in the prothoracicotropic hormoneproducing neurosecretory cells of the Bombyx mori brain ppt

... consist-ing of the ecdysone receptor (EcR) and ultraspiracle(USP) [7,8]. The 20E–receptor complex directly inducesseveral early genes and the products of these genesactivate late effector genes ... of the brains and analyzed by RT-PCR to assess the spatial distribution of gene expression. (C) RT-PCR analysis of EcR expression using RNA from the anterior (A) and posterior (P) part of the ... ultraspiracle (USP). Expression of the speciﬁc isoforms EcR-A and EcR-B1 governs steroid-induced responsesin the developing cells of the silkworm Bombyx mori. Here, analysis of EcR-A and EcR-B1 expression...

Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

... edetermined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic DNA ... fractionated on a 2% (w /v) agarose gel. The remainder of the PCR-ampliﬁed DNA was ligated intoTOPO-BluntTMvectorandusedtotransformTOP10cells(Invitrogen). The sequence of 20 individual clones wasdetermined ... youngHD mice. Loss of CB1 or other mRNAs and the development of NIIs and motor symptoms occur o vertime. I t is possible that, due to the postmitotic chan ge in the length of the CAG r epeat,...

Báo cáo khoa học: Differential expression pattern of the novel serine⁄threonine kinase, STK33, in mice and men doc

... for the negativecontrols. The resolution varied between 1 and 5 lmÆpixel)1depending on the size of the section of the image. The Adobe photoshop program was used to produce mergedimages in the ... testis, shows expression of STK33 in lung and heart. However,GeneCards expression data for STK33 are close to the background level, which is ﬁtting to the low level expression in tissues other than ... development checkpoint, which based on our dataseems suggestive, or rather reﬂects synchrony to otherevents of the spermatogenesis. For instance, the mech-anisms unleashing the pass of the...

Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc

... the qor genes and theirflanking regions. The plasmid pBG3a was digested with XhoIandDraIII to remove nptII. After the removal of the 3¢ overhang and the filling of the 5¢ overhang of the plasmid ... thatcleaves 364-bp upstream of the start codon of qorM ,and DraIII, that cleaves 8-bp downstream of the stop codon of qorL. After removing the 3¢ overhang and filling the 5¢overhang of the plasmid ... Fig. 4B. The absence of Mo (V) -signals reveals the g2-component of FeSII. It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and from P....

Xem thêm