Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx
Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx
... ª 2006 The Authors Journal compilation ª 2006 FEBS 2527 Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4Alejandra Sa´enz1,*, ... examine the interaction of KL4with POPG and PA as well as the effect of these lipids on the physical properties of the membrane.Effect of KL4on the lipid order of surfactant-like membranes To ... bilayers, and theirdependence on calcium. Therefore, the objectives of this study were to analyze (a) the effect of KL4on the physical properties of membranes, in the absence and presence of Ca2+,...
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Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx
... (a1/a2)andab regulatory subunit [9]. The enzymatic activity of all MATs studied depends on the redox state of the sulfhydryl moieties on the cysteineresidues in the amino acid sequence of the protein ... one-tenth of the activity displayed by the wild type. However, the ability of C92S, C106S and C47S tocleave PPPiremained high, and in the case of C47S, evenFig. 4. Time-course of L. donovanirecombinant ... mutated to their iso-sterical amino acid, serine. The C22S, C44S, C92S and C305Smutants showed a drastic loss of AdoMet synthesis activity compared to the wild type, and the C33S and C47S mutantsretained...
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Báo cáo khoa học: Methanoferrodoxin represents a new class of superoxide reductase containing an iron–sulfur cluster docx
... and wasabsent in the spectrum of the oxidized sample (datanot shown). The axial signal with g^ = 1.93 and gk = 2.047 was assigned to a [4Fe–4S] cluster. Thesefindings, together with those of ... amounts of MM0632. In contrast to the normal SODs, an excess of the protein from M. mazei caused reoxidation of reduced cytochrome c (Fig. 4). The enzymic activity of MM0632 was calculated on the ... by addition of rubredoxin. The SOR activity of MM0632 strongly depends on the presence of FNR, xan-thine oxidase and rubredoxin. No activity was observedwhen one of the proteins was omitted....
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Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf
... measuring the radioactivity of tritium, as described inMaterials and methods (In the figure, the y-axis represents the cpm values of pellet). Black and hatched bars represent the radio-activities of ... expressed as a per-centage of the maximum releasable Ca2+using the fluorescence and the radioactivity of 45Ca2+, and were compared with thosecaused by the addition of 1% Triton X-100 (TX-100), ... BI-1 and the InsP3receptor, we investigated the effect of externalCa2+on the PICR. External Ca2+consistently inhib-ited the PICR, regardless of the pH value outside the liposomes, when the...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
... with othercystatins, cystatin F is a rather slow binding inhibitor of the cathepsins, characterized by kassvalues in the range of 106)107m)1, and high kdissvalues in the range of 10)3to ... rather non-selective inhibitors. An interesting feature of cystatinF is the 100-fold stronger inhibition of cathepsins F,L, and V than of cathepsin S. Cathepsin S is closelyrelated to other ... protease activity, and not only abolish it [33]. These modulatoryinhibitors are often colocalized with their targets. Cyst-atin F could be a candidate for modulating the activity of cathepsin...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... min of reaction time, the yields of the trimer and the pentamer com-pared to the dimer were 34% and 30% for the wild-type, but 66% and 47% for the D392N mutant(Fig. 5B).Oligosaccharide synthesis ... adjusting the concentration of the enzymes toyield similar activity, the hydrolytic activities of the wild-type protein and of the D392N mutant wereassayed with (GlcNAc)2)6. As expected, the enzymesfailed ... quantities of the elongated oligomers. The maximum yields of (GlcNAc)5 and (GlcNAc)6, synthesized relatively to the hydrolytic product, (GlcNAc)2, were 3% and 9%for the wild-type enzyme and 11% and...
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Báo cáo khoa học: Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage /11 doc
... I in the presence of CI. Moreprecisely, the )21 to )48 and )52 to )87 regions of the top strand and )24 to )53 and )58 to )87 regions of the bottom strand were protected by CI (Fig. 3B). The centers ... affinity of CI to the two operators, and the mecha-nism of action of CI. In the present study, we report the purification of /11 CI to near homogeneity and, for the first time, present evidence for the ... contents in the shifted complexes 1 and 2 and in the unbound labeled O1O2 DNA were determined byscanning the intensities of all the bands shown in the autoradiogram of the gel shift assay (I) and plotted...
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Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf
... waspositive for the Ghana, Png and Uganda sequences. Position1278 was the only position of the alignment where botha-andb-O-GlcNAchavebeenpredictedintheGhana,Png and Uganda sequences. The Thr1503 ... enriched in the 19-kDa C-terminalfragments of MSP-1 and in the erythrocytic, GPI-linkedCD59. The Triton X-100 soluble fractions of the gradientcontained the bulk of the proteins displayed in the gradient,but ... potential close to the threshold line in the Thai and Wellcome sequences. All of these sites are located within the C terminus and correspondto the allelomorphic block 5–16 of the MSP-1 sequence...
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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-specific truncated splice variant Mark Wehner and Christian Herrmann doc
... manner, the entropic contributions of nucleotide binding and the coupled oligomerizationshow opposing signs. In the case of hGBP1, release of water from the nucleotide binding pocket and the pro-tein ... byhGBP5, and the enzymatic hydrolysis of GTP leads toGDP rather than GMP. The nucleotide affinities of hGBP5 are grossly similar to those of hGBP1, butthere are differences in thermodynamic and kineticdetails. ... transfer to the mant-group of the nucleotide) to minimize excitation of unbound nucleo-tide and thereby loss of signal quality. The traces werefitted using a single rate constant, and the resulting...
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Báo cáo khoa học: Conformational properties of bacterial DnaK and yeast mitochondrial Hsp70 Role of the divergent C-terminal a-helical subdomain pdf
... Mge1p were used in the sameconcentration as DnaJ and GrpE. Specific activity values (upper) and ratio of the Hsp70 ATPase activity in the presence of the speci-fied ligands to the activity without ... that of DnaK. The twofold reduction of the KSVvalues estimated formtHsp70 both in the absence and the presence of ATP(Table 2) supports the existence of differences in the Trp environment of ... KKCC and KCCCaffected the binding kinetics, the thermal stability of Hsp70 peptide complexes, and the refolding activity which might reflect a destabilization of the PBD.Conformational properties...
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