... 2006 The Authors Journal compilation ª 2006 FEBS 201
L. Acerenza and F. Ortega Metabolic control analysis of large responses
Modular metabolic control analysis of large responses
The general case ... measure the
changes in the intermediate and the demand rate and,
second, to perturb the demand module and measure
the changes in the inter...
... used.
Here, the general theory of modular MCA for large
responses is developed to include the analysis of
branched systems. This formalism enables the analysis
of systems with three modules and one explicit
intermediate. ... Ortega F (2007) Modular metabolic con-
trol analysis of large responses. The general case for
two modules and o...
... found
between the site of the no. tag D tag and a poly(dA)
among the cDNA of the gene (Hs. 436980). The blast
results of another 11 sequences in the GenBank
Table 1. Overview of all tags analyzed with the ... product of no. 10.
We further optimized the PCR annealing tempera-
ture, as well as the cycle number, for each of the
weak-band tags. Moreover, these...
... manner, the
actual composition of the spots depends on the com-
position of the lysates, the lysis buffer and the ‘affinity’
of the individual proteins for the solid phase. Varia-
tions in lysis and ... correlation
between the activation state, on the one hand, and the
biological or disease state, on the other hand.
Small molecules that modulate the ac...
... (3) their cores are such that they
are identical, or either is a member of the other in the
sense of a list member, or the left- or right-hand member
of either core is a member of the other. ... templates
The fourth formula is the proper qualifier for the first,
and, if such had been found for the second and third,
they would have appeared in place of the...
... interfaces of the
SQG and fatty acid separately. We then combined
these two interaction interfaces and identified the
SQMG interaction interface of the 8-kDa domain.
Analysis of the SQG interface with the ... amount of SQG or MA (i.e. I ⁄ P ¼ 10)
did not inhibit the ssDNA binding activity of the
8-kDa domain of pol b (Lanes 3 and 4 of Fig. 3C). On
the ot...
... to the scheme suggested by Duggleby [13,14]
in which E ¢ indicates the inactive form of the enzyme, and
J
1
, J
2
and J
3
are the rate constants for inactivation of the
respective forms of the ... in the course of the continuously
monitored reactions were analysed as described in the
Materials and methods. For the reactions in the
absence of fatty aci...
... seen as band doubling for T)11
and T+2. Therefore, the measured contribution of these
forms to the integrated intensity of these two bases was
used in the calculation of the contribution of individual
bases ... factor,
ox
k
i
,
Mg
⁄
ox
k
i
,
with the largest values of 4.4 for T+1 and 3.5 for
T+2, and much smaller, in the range 1.6–2.3, for the
most reac...
... essential for eukaryotic DNA
replication and are the most likely candidates for the
replicative DNA helicase responsible for unwinding
DNA at the replication forks [1–3]. Consistent with
their primary ... X-100 used
for cell fractionation and then incubated at 37 °C for
15 min in the same buffer before fixation (Figs 9 and 10).
Cells were washed with NaCl ⁄ P
i
and th...
... separately
for the numerator and the denominator of Eqn (5). Based
on the SD of the numerator and the denominator the SEM
of q
h
was computed, assuming statistical independence of
the two. The time-dependent ... contained
5 lL of SYBR Green PCR Core Kit (Bioke, Leiden, The
Netherlands), 3 pmol of each primer (Isogen, De Meern,
The Netherlands or Biolegio...