Báo cáo khoa học: A family of killer toxins Exploring the mechanism of ADP-ribosylating toxins pptx

Báo cáo khoa học: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin pot

Báo cáo khoa học: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin pot

... residues, vari- able oxidation of methionine and variable deamidation of asparagine and glutamine. Parent and fragment mass toler- ances were set to 1 Da. Up to two missed cleavages and half tryptic ... JD, Amanchy R, Kristiansen TZ, Jonnalagadda CK, Surendranath V, Niranjan V, Muthusamy B, Gandhi TK, Gronborg M et al. (2003) Development of human protein reference database as an initial...
Ngày tải lên : 07/03/2014, 06:20
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Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterfly, Papilio xuthus docx

... light adaptation of the eye. Light adaptation also induced the decrease of all-trans isomer both in the distal and proximal portions of the retina. In contrast, the increase of 11-cis ligand was ... using an ATTO AE6905C Image Saver, and quantified with NIH IMAGE program. The gel was then stained with Coomassie Brilliant Blue, and the protein content was measured via the...
Ngày tải lên : 17/03/2014, 03:20
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt

Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt

... ethanol, sonicated and extracted overnight at +4 °C [23]. After centri- fugation, the pellet was discarded and a sample of the supernatant was withdrawn for radioimmunoassay of insulin and glucagon ... Both end-plate potentials of the ion trap were set at 1.5 V and the duration of the electron pulse was 100 ms. Data acquisition and handling Primary data analysis was perfor...
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A pot

Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A pot

... activator of transcription 5A Yoshihisa Watanabe 1 , Masaya Ikegawa 2 , Yoshihisa Naruse 3 and Masaki Tanaka 1 1 Department of Cell Biology, Research Institute for Neurological Diseases and Geriatrics, ... suppresses the tran- scriptional activity of STAT 5A. Discussion We isolated a novel STAT 5A splicing variant from the mouse brainstem. The STAT 5A_ DE18 variant lacked th...
Ngày tải lên : 23/03/2014, 04:20
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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

... given the more recent studies, a re-examination of the role of Ras in the development of these cells seems to be war- ranted. With regards to transcriptional targets of the Itk pathway, although the ... factors nuclear factor for activated T cells (NFAT), nuclear factor kappa-light chain enhancer of activated B cells (NFjB) and activator protein-1 [4]. A number of st...
Ngày tải lên : 28/03/2014, 22:21
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Báo cáo khoa học: A family of killer toxins Exploring the mechanism of ADP-ribosylating toxins pptx

Báo cáo khoa học: A family of killer toxins Exploring the mechanism of ADP-ribosylating toxins pptx

... a manner similar to that of the catalytic glutamate of the ADPRT and this could be essential to the mech- anism and form part of the mechanism that has not yet been captured in a crystallographic ... M, Murata T, Nakayama K, Asakawa K, Ohara M, Komatsuzawa H & Sugai M (2001) Complete nucleotide sequence of a Staphylococcus aureus exfoliative toxin B plasmid and ide...
Ngày tải lên : 30/03/2014, 10:20
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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx

... or SYK themselves, is capable of transforming NIH-3T3 cells [70]. In addition, we and others have demonstrated that the activation and plasma membrane localization of the fusion construct are dependent ... F, Cazzola G, Consolini R, De Mattia D, Dell’Erba G et al. (2002) Clinical, immunological, and molecular analysis in a large cohort of patients with X-linked agammaglobulinemia:...
Ngày tải lên : 14/02/2014, 18:20
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Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf

... 5¢-GTTCACCACCAGAAGCT GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab- start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3¢;Abstop, ... 5¢-CCTGCCGAGCTCCTATTA CACAACGCCACCAACCATCAG-3¢. The PCR solution was prepared in the buffer supplied with the enzyme, and contained Aba, Abb and Abcat 40 nm each, and the start and stop...
Ngày tải lên : 18/02/2014, 13:20
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

... the performance of the potential will only increase with the size of the structural database and as terms are added to the model to account for protein and RNA intra-molecular interactions that are ... Given the relative sparsity of the structural database, we investigated whether a reduced-atom representation would not lead to improved performance in the protein–RNA c...
Ngày tải lên : 18/02/2014, 16:20
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Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx

Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx

... [46]. The appear- ance of the LIRs probably provides humans with an evolutionary advantage and contributes to the specia- tion of primates. Experimental procedures Identification of LIRs The human ... termed arms) that are approximately complemen- tary to each other. Generally, there is a spacer between the arms, and the full structure of an inverted repeat can form a s...
Ngày tải lên : 07/03/2014, 00:20
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