... residues, vari-
able oxidation of methionine and variable deamidation of
asparagine and glutamine. Parent and fragment mass toler-
ances were set to 1 Da. Up to two missed cleavages and
half tryptic ... JD, Amanchy R, Kristiansen TZ,
Jonnalagadda CK, Surendranath V, Niranjan V,
Muthusamy B, Gandhi TK, Gronborg M et al. (2003)
Development of human protein reference database as
an initial...
... light adaptation of the eye. Light adaptation also
induced the decrease of all-trans isomer both in the distal
and proximal portions of the retina. In contrast, the increase
of 11-cis ligand was ... using an ATTO AE6905C
Image Saver, and quantified with
NIH IMAGE
program. The
gel was then stained with Coomassie Brilliant Blue, and
the protein content was measured via the...
... ethanol, sonicated
and extracted overnight at +4 °C [23]. After centri-
fugation, the pellet was discarded and a sample of the
supernatant was withdrawn for radioimmunoassay of
insulin and glucagon ... Both end-plate potentials of the ion
trap were set at 1.5 V and the duration of the electron
pulse was 100 ms.
Data acquisition and handling
Primary data analysis was perfor...
... activator of
transcription 5A
Yoshihisa Watanabe
1
, Masaya Ikegawa
2
, Yoshihisa Naruse
3
and Masaki Tanaka
1
1 Department of Cell Biology, Research Institute for Neurological Diseases and Geriatrics, ... suppresses the tran-
scriptional activity of STAT 5A.
Discussion
We isolated a novel STAT 5A splicing variant from
the mouse brainstem. The STAT 5A_ DE18 variant
lacked th...
... given the
more recent studies, a re-examination of the role of
Ras in the development of these cells seems to be war-
ranted. With regards to transcriptional targets of the
Itk pathway, although the ... factors nuclear factor for activated
T cells (NFAT), nuclear factor kappa-light chain
enhancer of activated B cells (NFjB) and activator
protein-1 [4]. A number of st...
... a
manner similar to that of the catalytic glutamate of
the ADPRT and this could be essential to the mech-
anism and form part of the mechanism that has not
yet been captured in a crystallographic ... M,
Murata T, Nakayama K, Asakawa K, Ohara M,
Komatsuzawa H & Sugai M (2001) Complete nucleotide
sequence of a Staphylococcus aureus exfoliative toxin B
plasmid and ide...
... or SYK themselves, is capable
of transforming NIH-3T3 cells [70]. In addition, we
and others have demonstrated that the activation and
plasma membrane localization of the fusion construct
are dependent ... F, Cazzola G, Consolini R, De Mattia D,
Dell’Erba G et al. (2002) Clinical, immunological, and
molecular analysis in a large cohort of patients with
X-linked agammaglobulinemia:...
... 5¢-GTTCACCACCAGAAGCT
GGTGTTCTTC GCTGAA GACGT GGGTTCT AACAAG
GGTGCT-3¢;Abc, 5¢-CACAACGCCACCAACCATCAGA
CCGATGATAGCACCCTTGTTAGAACCCAC-3¢;Ab-
start, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT
CCGTCACG-3¢;Abstop, ... 5¢-CCTGCCGAGCTCCTATTA
CACAACGCCACCAACCATCAG-3¢.
The PCR solution was prepared in the buffer supplied
with the enzyme, and contained Aba, Abb and Abcat
40 nm each, and the start and stop...
... the
performance of the potential will only increase with
the size of the structural database and as terms are
added to the model to account for protein and RNA
intra-molecular interactions that are ... Given the relative
sparsity of the structural database, we investigated
whether a reduced-atom representation would not lead
to improved performance in the protein–RNA c...
... [46]. The appear-
ance of the LIRs probably provides humans with an
evolutionary advantage and contributes to the specia-
tion of primates.
Experimental procedures
Identification of LIRs
The human ... termed arms) that are approximately complemen-
tary to each other. Generally, there is a spacer between
the arms, and the full structure of an inverted repeat
can form a s...