... down-regulating vimentin in the absence and in the presence of MTX. Targeting of vimentinwas carried out by lipofecting aODN-VIM into paren-tal HT29 cells causing a decrease of 60% in theexpression of ... (IUPUI, Indianapolis, IN, USA), or the TOP1 inhibitor camptothecin (Sigma,Madrid, Spain), in the presence or absence of MTX. IMPDHactivity inhibitors were added simultaneously to MTX.Camptothecin, ... collected,washed in NaCl ⁄ Pi and resuspended in annexin-binding buf-fer. Cells were collected, washed in NaCl ⁄ Pi and resuspended in binding buffer containing a final concentration of 0.5 lgÆmL)1annexin...