Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

Tài liệu Báo cáo khoa học: Hepatic stimulator substance mitigates hepatic cell injury through suppression of the mitochondrial permeability transition pdf

... decreasing the activity of caspase-3 in the three cell lines.Effect of HSS knockdown on CCCP-inducedapoptosisTo further elucidate the functional role of HSS inCCCP-induced apoptosis, the expression ... to the uncoupling of oxidativephosphorylation. MPT is caused by the opening of the nonselective, highly conductive PTP in the mitochon-drial IM [39]. The exact molecular composition of the PTP ... mitochondria-dependent apoptosis [12], the assembly of the apoptosome. Upon formation of this complex, caspase-9 acquires the ability to trigger the processing and activation of the downstream cas-pase...

Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx

... [30]. Thus, the role of L-PGDSin adipogenesis and diabetes-related phenotypes is not clear. Moreover, because of the dual functions of L-PGDS, whether PGD2regulates the differentiation of adipocytes ... distribution of adipose tissues asdetermined by computed tomography (C T) analysisTo further assess the effect of H-PGDS overexpressionon the increase in adipose tissues, the weights of subcutaneous ... subcutaneous and visceral adi-pose tissues were increased in TG mice by the HF diet.mRNA expression of adipogenic genes in WAT of TG miceWe measured the amounts of PGD2in WAT after6 weeks of the HF...

Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt

... in the AP2 domain of RAP2.4 does not affect assembly with BPMs, this does not ruleout a possible role of the domain for this type of interaction. However, to date, there is no evidencethat the ... expression in 3-day-old seedlings in cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves werestained in the vascular tissue of ... but not in parts of the hypocotyls (Fig. 4G). In rosette leaves, weobserved expression in the vascular tissue of the leafblade, whereas, interestingly, in older parts of the mid-rib, no GUS expression...

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... of PHI to either the complex PHR-PHO or to PHR alone, in the stoichiometry ratioPHR : PHO : PHI 2 : 1 : 1 (Fig. 5). On the contrary, the emission spectrum of PHO-bound ANS is not affected by the ... The absence of redox centres (FAD, Fe/S) excludes the ﬁrsthypothesis and therefore a direct involvement of PHI inelectron transfer. This conclusion is consistent with the verylow degree of identity ... possibility is that PHI interacts with onePHR and one PHO (abc) protomer. The hypothesis of adirect PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not...

Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf

... occurs in the cellwhenever the degradative capacity of the proteasome is exceeded. It is thought to be a symptom of saturation of the UPS [12,13]. To verify whether the expression of aggregating ... release and activation of caspase 3 (data not shown). This appears to be a general mechanism, which is not dependent on the presence of the intracellular antigen orformation of the antigen–antibody ... The migration of molecular mass markers (in kDa) and of the heavy (c) and light (k) IgG chains are indicated. Arrows point to the scPs, and the asterisks denote three ubiquitinated bands of the...

Báo cáo khoa học: a-Fetoprotein positively regulates cytochrome c-mediated caspase activation and apoptosome complex formation docx

... sup-pression of apoptosis and enhancement of the malignanceand therapy resistance of tumors [39–43]. The existence of ahigh background level of antiapoptotic factors in the cytosol of tumor cells often ... cancel their resistance to apoptosis. There are severalhypotheses that may help to explain these ﬁndings. The release of cyt-c from the mitochondria into the cytosol hasbeen shown to be one of the ... Arrowheads at the top of the patterns indicate sizes of calibration protein standards and their elution positions from the Superose-6 column.Dot-blot analysis of the AFP distribution in the fractions...

Báo cáo khoa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis – catalytic sites of the homodimeric enzyme are functional and regulated docx

... dissociation–association. In the absence of this information, Scheme 1 is not valid. Subse-quently, we veriﬁed whether the Michaelis–Mentenrelationship is or is not maintained by the enzyme.When product ... case of inactivation by trypsin, the proﬁle distinctlyindicated the existence of a stable intermediate between0.1 mm and 0.2 mm of the inhibitor. This intermediateretained 58% ± 2% of the residual ... inhibitor-free and native epimerase is an indication of the regulatory role of the bound5¢-UMP in the latter. The Michaelis–Menten relation-ship of the monomeric epimerase displays the usualmonophasic hyperbolic...

Báo cáo khoa học: 2-Oxo acid dehydrogenase complexes in redox regulation Role of the lipoate residues and thioredoxin pot

... the inactivation. This is in good accord with the independence of the inactivation on the presence of oxygen. The dihydrolipoate -mediated inactivation of E1 at lowNAD+concentrations is more ... Participation of the 2-oxo acid dehydrogenase complexes in the redox regulation of metabolism. The redox state of the surroundingmedium is sensed by the complexes through the concentrations of 2-oxo ... domains cannot be ascribed entirely to the catalytic role of these domains. Rather, the advantageappears to depend on the ability of the lipoyl group toprotrude from the inner core of the complex,...

Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx

... rich resource for the discovery of novel pharmaceutical agents. The biosyn-thesis of the oligopeptides can be either carried out viaa ribosomal strategy, as in the case of capistruin orpatellamide, ... Encouraged by the results obtained,and to investigate the mechanism of macrocyclization, the steric demand of the C-terminal amino acid wasfurther increased by the incorporation of l-Met4(TL4) ... (Fig. 2).Due to the fact that the number of amino acidsfound within the product does not correlate with the total number of adenylation domains, an iterativemechanism of biosynthesis was proposed...

Báo cáo khoa học: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of b-oxidation doc

... analysis system LeicaQ500MC (Leica).Analysis of fatty acid and TG metabolismTo assess the effect of inhibition of fatty acid synthesis onTG accumulation, THP-1 macrophages infected with eitherAd.CMV.adipophilin ... The mechanism of TG accumulation in humanmonocyte–macrophages primarily involves the directuptake of free fatty acids generated by the extracellularlipoprotein lipase -mediated hydrolysis of ... stimulation of fatty acid oxidation by AICARappears to be dominant over the inhibition of fattyacid oxidation by adipophilin.DiscussionWe studied the impact of adenoviral -mediated overex-pression of...

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